关键词: 水稻白叶枯病, 抗病相关基因, 亚细胞定位, sGFP

Abstract: The subcellular localization of rice bacterial blight related genes were examined to provide important information for the study of gene function. Eighteen candidate genes which potentially associated with bacterial blight resistance were selected from Y73 by using gene chip, semiquantitative/quantitative PCR and function annotation. These candidate genes were fused with green fluorescent marker (sGFP) and subcellular localization of their products in tobacco leaf cells. Four genes combined with sGFP were found mainly located in the cell nucleus, showing the typical characteristics of transcription factors; Four genes were found mainly located in the plasma membrane, indicating their potential functions of maintaining the stability of the cell membrane or in transport control on cell membrane; Four genes were simultaneously located in both cell nucleus and the plasma membrane and there was no apparent subcellular localization signal for the remaining 6 genes combined with sGFP.

Key words: rice bacterial blight, resistance genes, subcellular localization, sGFP