浙江农业学报

• 环境科学 • 上一篇    下一篇

高效去除镉污染真菌的筛选与鉴定

  

  1. (湖南农业大学 生物科学技术学院,湖南 长沙 410128)
  • 出版日期:2015-04-25 发布日期:2015-04-30

Screening and identification of high\|efficiency cadmium\|removing fungus

  1. (College of Bioscience and Biotechnology, Hunan Agriculture University, Changsha 410128, China)
  • Online:2015-04-25 Published:2015-04-30

摘要: 为获得高效去除镉污染的菌株,采用多点随机法采集镉污染土壤,通过稀释平板法初筛耐Cd2+真菌,将筛得的菌株分别接种于含不同浓度Cd2+的PDA固体培养基上进行复筛,进行发酵试验检测各菌株对Cd2+的去除能力。结果表明,初筛共分离获得9株真菌(依次命名为PC\|1至PC\|9),其中,菌株PC\|8能在含32 mmol·L-1镉的培养基上正常生长,对Cd2+的去除效率最高。遗传稳定性试验表明,该菌株的耐镉性能是由遗传性状控制的。经形态学观察和ITS序列同源性比对,将该菌株鉴定为新月弯孢霉菌(Curvularia lunata)。

关键词: 耐镉菌株, 真菌, 分离, 鉴定, 新月弯孢菌

Abstract: In order to obtain strains able to efficiently removing cadmium, cadmium\|contaminated soil samples were collected via random sampling method. The first screening test of cadmium\|resistant strains was carried out through pour plate method, and then the selected stains were inoculated in PDA solid medium containing different concentrations of cadmium for further screening. The removal capacities of cadmium\|resistant strain were determined through fermentation tests. It was shown that nine fungi were isolated within the first screening, and were named as PC\|1 to PC\|9 accordingly. In the heavy metal concentration gradients and fermentation tests, strain PC\|8 exhibited the highest Cd\|removing efficiency, as it grew well in the PDA solid medium containing 32 mmol·L-1 Cd2+. Genetic stability tests proved that the cadmium\|resistance trait of PC\|8 was controlled by genetic character. Based on morphological observation and ITS sequence homology comparison, the strain PC\|8 was primarily identified as Curvularia lunata.

Key words: Cd\, resistant strain;fungus;screening;identification;Curvularia lunata