浙江农业学报

• 作物科学 • 上一篇    下一篇

大巴山粉葛组织培养技术

  

  1. (1.四川农业大学 林学院,四川 成都 611130; 2.四川省三台县林业局,四川 三台 621100)
  • 出版日期:2016-07-25 发布日期:2016-07-08

Tissues culture of Pueraria lobata (wild) Ohwi Dabashan

  1. (1. College of Forestry, Sichuan Agricultural University, Chengdu 611130, China; 2. Forestry Bureau of Santai County, Santai 621100, China)
  • Online:2016-07-25 Published:2016-07-08

摘要: 为了实现大巴山粉葛Pueraria lobata (wild) Ohwi Dabashan种苗的规模化快速繁殖,以大巴山粉葛茎段为外植体,研究了灭菌时间、MS培养基大量元素、植物生长调节剂对大巴山粉葛组织培养过程中消毒、初代培养、增殖培养及生根培养的影响。结果表明,(1)先用75%乙醇消毒20 s,再用0.1%氯化汞消毒10 min,成活率达67.56%。(2)初代培养基为1/2 MS+0.5 mg·L-1 6-BA+0.3 mg·L-1 IBA,腋芽萌发率可达92.2%;(3)增殖培养基为1/2 MS+1.0 mg·L-1 6-BA+0.05 mg·L-1 IBA,增殖系数约5.0;(4)生根培养基为1/2 MS+0.01 mg·L-1 NAA+0.1 mg·L-1 IBA,生根率达96.03%。

关键词: 大巴山粉葛, 组织培养, 腋芽萌发, 植物生长调节剂

Abstract: In order to realize the rapid propagation of Pueraria lobata(wild) Ohwi Dabashan, the effects of sterilization time, macroelement in MS medium and plant growth regulators on disinfection, initial culture, multiplication culture and rooting culture of Pueraria lobata(wild) Ohwi Dabashan were studied by tissues culture, using its stem with axillary bud as explants. The results showed that: (1) The explants were sterilized in 75% alcohol and 0.1% HgCl2 for 20 s and 10 min, the survival rate reached to 67.56%. (2) The medium for the initial culture was 1/2 MS+0.5 mg·L-1 6-BA+0.3 mg·L-1 IBA, the percentage of axillary bud was up to 92.2%. (3) The optimum multiplication medium was 1/2 MS+1.0 mg·L-1 6-BA+0.05 mg·L-1 IBA, multiplication coefficient reached to 5.0. (4) The rooting medium was 1/2 MS+0.01 mg·L-1 NAA+0.1 mg·L-1 IBA, with rooting rate 96.03%.

Key words: Pueraria lobata(wild) Ohwi Dabashan, tissues culture, axillary bud sprouting, plant growth regulator