浙江农业学报

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三个大豆品种萌发期和苗期的耐盐性比较

  

  1. (1.浙江师范大学 化学与生命科学学院,浙江 金华 321004; 2.浙江省农业科学院 作物与核技术利用研究所,浙江 杭州 310021; 3.长江大学 园艺园林学院,湖北 荆州 434023)
  • 出版日期:2016-07-25 发布日期:2016-07-08

Research on salt tolerance of three soybean varieties at both germination and seedling stage

  1. 1. College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321004, China; 2. Institute of Corp and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China; 3. College of Horticulture and Garden, Yangtze University, Jingzhou 434023, China)
  • Online:2016-07-25 Published:2016-07-08

摘要: 以3个大豆品种冀豆12、浙春4号和天隆一号为试验材料,分别研究了NaCl(盐)处理对种子萌发和大豆幼苗植株生长的影响。种子萌发试验表明,高浓度盐处理显著抑制种子发芽,且不同品种之间差异显著,冀豆12表现相对较好。大豆2叶1心期,以100 mmol·L-1 NaCl浇施大豆幼苗1次,2 d后重复1次,并测定抗逆相关生理指标,结果表明,盐胁迫下大豆的叶绿素含量、根系活力、干质量均有所降低;丙二醛含量、过氧化物酶活性、超氧化物歧化酶活性、游离脯氨酸含量均升高,不同品种各指标的升降幅度各不相同。综合种子萌发及苗期耐盐性,试验初步判断冀豆12比其他2个品种更耐盐。

关键词: 大豆, 盐胁迫, 萌发, 苗期, 生理指标

Abstract: Three soybean varieties i.e. Jidou 12, Zhechun No.4 and Tianlong No.1 were used in this research to study the effects of salt treatment on soybean seed germination and seedling growth. Results showed that high concentration of salt significantly inhibited the seed germination, and the inhibitory effects varied significantly among varieties. Soybean seedlings were irrigated with 100 mmol·L-1 NaCl 1 time at twoleavesoneshoot stage and after 2 days, respectively. Salt stress conditions, leaf chlorophyll content, root activity and dry mass weight were all decreased, the MDA content, POD activity, SOD activity and free proline content were increased, and the range of variations varied among soybean varieties. The data from both seed germination experiment and seedling experiments inferred that Jidou 12 had better salt tolerant ability than the others.

Key words: soybean, salt stress, germination, seedling, physiological index