圈养林麝养殖场内铜绿假单胞菌的分布及其耐药性分析

doi:10.3969/j.issn.1004-1524.2016.11.07

浙江农业学报 ›› 2016, Vol. 28 ›› Issue (11): 1847-1852.DOI: 10.3969/j.issn.1004-1524.2016.11.07

圈养林麝养殖场内铜绿假单胞菌的分布及其耐药性分析

郗立新¹, 文彩芳¹, 赵珊¹, 陈珍容¹, 卓利苗¹, 杨光友¹, 颜其贵^{1, 2, *}, 郑从军³, 王洪永⁴, 夏中林⁵

1.四川农业大学动物医学院,四川成都 611130;
2.四川农业大学动物疫病与人类健康四川省重点实验室,四川成都 611130;
3.四川省宝兴县林业局,四川雅安 625700;
4.四川夹金山逢春养殖科技有限公司,四川德阳 618100;
5.四川省峨眉山市林业管理局,四川峨眉山 614200

收稿日期:2016-04-13 出版日期:2016-11-15 发布日期:2016-11-16
通讯作者: 颜其贵,E-mail:yanqigui@126.com
作者简介:郗立新(1991—),男,陕西凤翔人,硕士研究生,研究方向为兽医微生物与免疫学。E-mail:13547480554@163.com
基金资助:
四川省科技支撑计划项目(2014SZ0132)

Distribution-survey of Pseudomonas aeruginosa of captive forest musk deer and analysis of drug resistance

XI Li-xin¹, WEN Cai-fang¹, ZHAO Shan¹, CHEN Zhen-rong¹, ZHUO Li-miao¹, YANG Guang-you¹, YAN Qi-gui^{1, 2, *}, ZHENG Cong-jun³, WANG Hong-yong⁴, XIA Zhong-lin⁵

1. College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China;
2. Animal Epidemic Disease and Human Health Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China;
3. Sichuan Baoxing County Forestry Bureau, Ya’an 625700, China;
4. Sichuan Feng-chun Breeding Technoloyg Co., Ltd, Deyang 618100, China;
5. E’meishan Forestry Administration Bureau of Sichuan Province, E’meishan 614200, China

Received:2016-04-13 Online:2016-11-15 Published:2016-11-16

摘要/Abstract

摘要： 从陕西镇坪、四川宝兴两个林麝养殖中心共采集124份样品进行铜绿假单胞菌的分离鉴定,并采用微量肉汤稀释法检测其耐药情况。结果显示,共分离出60株铜绿假单胞菌,其中镇坪34株,宝兴26株。两个养殖场铜绿假单胞菌检出率由高到低依次为伤口脓汁>死亡林麝组织器官>伤皮>鼻拭子>土壤>粪便>尿液>空气,且镇坪比宝兴的总检出率高。所分离的60株铜绿假单胞菌耐药表型趋于一致,都对阿米卡星、庆大霉素、环丙沙星敏感,对亚胺培南中等敏感,对多西环素、四环素、磺胺异恶唑严重耐药。

关键词: 林麝, 铜绿假单胞菌, 分离鉴定, 药敏

Abstract: A total of 124 samples were collected from Zhenping Country, Shaanxi Province and Baoxing Country, Sichuan Province, and were used to isolate and identify Pseudomonas aeruginosa, as well as to test their drug resistance by traditional bacteriological identification methods. It was shown that 60 P. aeruginosa strains were isolated, of which 34 were isolated from Zhenping, and 26 were isolated from Baoxing. The detection rate decreased as wound pus>dead deer tissues and organs>skin wounds>nasal swabs>soil>feces>urine>air. The detection ratio of Zhenping samples was higher than that of Baoxing. All 60 isolated P. aeruginosa strains exhibited similar resistance phenotypes. All strains were sensitive to amikacin, gentamicin, ciprofloxacin, and were moderately sensitive to imipenem, but were resistance to doxycycline, tetracycline, sulphamethoxazole.

Key words: forest musk deer, Pseudomonas aeruginosa, isolation and identification, susceptibility

中图分类号:

S855.1⁺2

郗立新, 文彩芳, 赵珊, 陈珍容, 卓利苗, 杨光友, 颜其贵, 郑从军, 王洪永, 夏中林. 圈养林麝养殖场内铜绿假单胞菌的分布及其耐药性分析[J]. 浙江农业学报, 2016, 28(11): 1847-1852.

XI Li-xin, WEN Cai-fang, ZHAO Shan, CHEN Zhen-rong, ZHUO Li-miao, YANG Guang-you, YAN Qi-gui, ZHENG Cong-jun, WANG Hong-yong, XIA Zhong-lin. Distribution-survey of Pseudomonas aeruginosa of captive forest musk deer and analysis of drug resistance[J]. , 2016, 28(11): 1847-1852.

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圈养林麝养殖场内铜绿假单胞菌的分布及其耐药性分析

Distribution-survey of Pseudomonas aeruginosa of captive forest musk deer and analysis of drug resistance

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