四川红原县牦牛隐孢子虫感染情况分子流行病学调查

doi:10.3969/j.issn.1004-1524.2016.11.06

浙江农业学报 ›› 2016, Vol. 28 ›› Issue (11): 1842-1846.DOI: 10.3969/j.issn.1004-1524.2016.11.06

四川红原县牦牛隐孢子虫感染情况分子流行病学调查

郝力力¹, 李锐², 段玲¹, 贺亮^{3, *}

1.西南民族大学生命科学与技术学院,四川成都 610041;
2.浙江省农业科学院农产品质量标准研究所,浙江杭州310021;
3.国家上海新药安全评价研究中心,上海201203

收稿日期:2016-03-04 出版日期:2016-11-15 发布日期:2016-11-16
通讯作者: 贺亮,E-mail:microvet@163.com
作者简介:郝力力(1980—),男,山西长治人,博士,副研究员,研究方向为分子寄生虫学。E-mail:leelee_hao@126.com
基金资助:
西南民族大学中央高校基本科研业务费青年教师基金项目(2016NZYQN34); 国家科技支撑项目(2014BAD13B03)

Prevalence and molecular identification of Cryptosporidium spp. in yaks in Hongyuan County of Sichuan Province

HAO Li-li¹, LI Rui², DUAN Ling¹, HE Liang^{3, *}

1. College of Life Science & Technology, Southwest University for Nationalities, Chengdu 610041, China;
2. Institute of Quality and Standard for Agro-products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;
3. National Shanghai Center for New Drug Safety Evaluation and Research, Shanghai 201203, China

Received:2016-03-04 Online:2016-11-15 Published:2016-11-16

摘要/Abstract

摘要： 为调查红原地区牦牛隐孢子虫感染情况,采用巢式PCR对采自红原县的216头份牦牛粪便进行了检测(其中包括腹泻粪便样本18份)。该地区牦牛隐孢子虫总感染率为14.8%,其中Cryptosporidium bovis 感染率为3.7%,C. ryanae感染率为5.1%, C. andersoni感染率为6.0%, 而腹泻样本并未检出隐孢子虫。以上结果表明,牦牛是隐孢子虫的天然宿主,但该实验中未见隐孢子虫和牦牛腹泻间存在明显相关性,隐孢子虫和牦牛腹泻之间的关系需要后续研究来进一步阐明。

关键词: 牦牛, 隐孢子虫, 腹泻

Abstract: In order to investigate prevalence of Cryptosporidium spp. in yaks in Hongyuan County, in this study, Cryptosporidium isolates from 216 fecal samples were identified by nest-PCR method (including 18 diarrhea fecal samples). Total infection rate of Cryptosporidium was 14.8%. It showed that prevalence of Cryptosporidium bovis, C. ryanae and C. andersoni was 3.7%, 5.1% and 6.0%, respectively. However, no Cryptosporidium spp. was detected in 18 diarrhea fecal samples. Above results showed that yaks were natural hosts of Cryptosporidium spp.. But in this study, no obvious correlation existed between Cryptosporidium spp. and diarrhea, and the relationship between Cryptosporidium spp. and diarrhea in yaks should be further explored.

Key words: yaks, Cryptosporidium spp., diarrhea

中图分类号:

S855.9⁺9

郝力力, 李锐, 段玲, 贺亮. 四川红原县牦牛隐孢子虫感染情况分子流行病学调查[J]. 浙江农业学报, 2016, 28(11): 1842-1846.

HAO Li-li, LI Rui, DUAN Ling, HE Liang. Prevalence and molecular identification of Cryptosporidium spp. in yaks in Hongyuan County of Sichuan Province[J]. , 2016, 28(11): 1842-1846.

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四川红原县牦牛隐孢子虫感染情况分子流行病学调查

Prevalence and molecular identification of Cryptosporidium spp. in yaks in Hongyuan County of Sichuan Province

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