五苓散对实验性铜中毒大鼠的治疗作用

doi:10.3969/j.issn.1004-1524.2017.02.05

浙江农业学报 ›› 2017, Vol. 29 ›› Issue (2): 206-212.DOI: 10.3969/j.issn.1004-1524.2017.02.05

五苓散对实验性铜中毒大鼠的治疗作用

程颜¹, 刘亚云¹, 张方洁¹, 黄翠华¹, 康佑汐¹, 韩春杨¹, 陈玎玎^{2, *}, 刘翠艳^{1, *}

1. 安徽农业大学动物科技学院,安徽合肥 230036;
2. 安徽农业大学生物技术中心,安徽合肥 230036

收稿日期:2016-09-09 出版日期:2017-02-15 发布日期:2017-03-06
通讯作者: 陈玎玎,E-mail:dingding0626@126.com;刘翠艳,E-mail:cyliu@ahau.edu.cn
作者简介:程颜(1995—),女,安徽宣城人,本科生,动物医学专业。E-mail:410150915@qq.com
基金资助:
国家自然科学基金资助项目(31172358); 2015年国家级大学生创新训练项目(201510364008)

Effect of Wuling San on copper poisoning in experimental rats

CHENG Yan¹, LIU Yayun¹, ZHANG Fangjie¹, HUANG Cuihua¹, KANG Youxi¹, HAN Chunyang¹, CHEN Dingding^{2, *}, LIU Cuiyan^{1, *}

1. College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China;
2. Biotechnology Center, Anhui Agricultural University, Hefei 230036, China

Received:2016-09-09 Online:2017-02-15 Published:2017-03-06

摘要/Abstract

摘要： 为探讨中药利湿方剂五苓散对动物体内铜排泄的影响,通过饲喂高铜饲料建立SD大鼠亚慢性铜中毒病理模型,然后用五苓散进行治疗。分别在造模成功和治疗结束时进行心脏采血,火焰原子吸收法测定大鼠血液铜含量,试剂盒法测定血清中AST、LDH和SOD的活性;试验结束时剖杀大鼠,观察内脏器官的病理变化,并取肝脏、肾脏进行组织病理学检查。造模结束时检查发现,与对照组相比,模型组大鼠体质量增长速度减缓,肝脏和肾脏出现明显的病理学变化,血液铜含量极显著升高(P<0.01),说明造模成功。采用五苓散治疗后,与模型组比较,治疗组大鼠肝肾病理学变化明显减轻,血液中铜含量显著降低(P<0.05);SOD活性升高(P<0.05),AST和LDH活性降低(P<0.05);与空白对照组比较,治疗组大鼠上述指标均无显著差异(P>0.05)。上述结果表明,五苓散能有效促进铜中毒大鼠体内铜的排泄,减轻肝、肾组织损伤,可用于临床动物亚慢性铜中毒的治疗。

关键词: 五苓散, 大鼠, 铜中毒, 治疗

Abstract: In order to investigate the effects of Wuling San, a Chinese herbal medicine compound powder with promoting diuresis, on the copper excretion in animal, the pathological model of subchronic copper poisoning in SD rats was established by feeding high copper diet, and then Wuling San as a therapeutic drug was given by means of intragastric administration to rats. After the success of building models and at the end of treatment, the blood sample of rats was collected by cardiac puncture for determining copper content with the method of flame atomic absorption spectrometry, and the activities of serum AST, LDH and SOD were detected by reagent kit. At the end of the experiment, all the rats were anatomized to observe the changes of pathologic autopsy of the internal organs, and the histopathologic changes of liver and kidney. Results showed that compared with control group, the rats of the model group were slow in velocity of body weight increasement, the obvious pathological changes of liver and kidney structure were found and the level of blood copper was significantly higher (P<0.01), indicating that the model of subchronic copper poisoning in rats was successfully established. After treated with Wuling San, compared with model group, the treatment group had less detectable changes in histopathology, the level of blood copper was significantly lower (P<0.05), SOD activity increased (P<0.05) and AST and LDH activities declined (P<0.05). The above five indexes had no significant differences between the treatment group and the control group (P<0.05). The results of this study indicated that Wuling San could effectively promote the excretion of copper in rats, alleviate the damage of liver and kidney, so it could be used to treat clinical subchronic copper poisoning of animals.

Key words: Wuling San, rat, copper poisoning, therapy

中图分类号:

S853.91

程颜, 刘亚云, 张方洁, 黄翠华, 康佑汐, 韩春杨, 陈玎玎, 刘翠艳. 五苓散对实验性铜中毒大鼠的治疗作用[J]. 浙江农业学报, 2017, 29(2): 206-212.

CHENG Yan, LIU Yayun, ZHANG Fangjie, HUANG Cuihua, KANG Youxi, HAN Chunyang, CHEN Dingding, LIU Cuiyan. Effect of Wuling San on copper poisoning in experimental rats[J]. , 2017, 29(2): 206-212.

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五苓散对实验性铜中毒大鼠的治疗作用

Effect of Wuling San on copper poisoning in experimental rats

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