浙江农业学报 ›› 2017, Vol. 29 ›› Issue (4): 575-582.DOI: 10.3969/j.issn.1004-1524.2017.04.09

• 园艺科学 • 上一篇    下一篇

姜荷花种球抗寒生理生化特征及促抗寒药剂效果

刘建新, 徐笑寒, 丁华侨   

  1. 浙江省农业科学院 花卉研究开发中心,浙江 杭州 311202
  • 收稿日期:2016-11-03 出版日期:2017-04-20 发布日期:2017-04-27
  • 作者简介:刘建新(1980—),男,湖南祁阳人,助理研究员,博士,主要从事花卉育种和栽培研究。E-mail:liujianxin2000@aliyun.com
  • 基金资助:
    浙江省农业科学院青年人才培养项目(重大课题主持能力培养类); 浙江省农业科学院农业科技发展专项; 浙江省农业科学院科技产业化资金项目; 杭州市科技计划引导项目(20163501Y78)

Physiological and biochemical characteristics of Curcuma alismatifolia bulbs and effects of some chemicals on its cold resistance

LIU Jianxin, XU Xiaohan, DING Huaqiao   

  1. Research and Development Centre of Flower, Zhejiang Academy of Agricultural Sciences, Hangzhou 311202, China
  • Received:2016-11-03 Online:2017-04-20 Published:2017-04-27

摘要: 姜荷花是国内新兴的一种热带花卉,但中国绝大部分地区因冬季寒冷导致种球不能自然越冬保存。为了明确姜荷花种球的抗寒生理,以便找出进一步提升种球抗寒性的方法,用不同低温胁迫处理姜荷花清迈粉种球,分析其各种生理生化指标,并开展了种球的抗寒药剂筛选试验。结果表明,姜荷花种球在9 ℃开始表现出逆境胁迫;由抗氧化酶系统和渗透调节系统的变化可知,种球分别在5和1 ℃调动了自身的抗逆系统来抵御环境的不良影响,在-3 ℃,各抗逆相关指标基本上降低到最低点。抗寒药剂筛选试验表明,各药剂处理(包括脱落酸、水杨酸、芸苔素内酯、6-BA、多效唑等)均对增强姜荷花种球的抗寒性有一定作用。根据细胞受损程度分析,1 mg·L-1 ABA浸泡种球处理24 h的保护效果最好;其次,0.08 mg·L-1 6-BA处理对膜透性有较好的保护作用,但膜脂的氧化程度较高。

关键词: 姜荷花, 清迈粉, 种球, 抗寒性, 脱落酸

Abstract: Research and Development Centre of Flower, Zhejiang Academy of Agricultural Sciences, Hangzhou 311202, China)Abstact: Curcuma alismatifolia is a new kind of tropical flowers. However, winter in most areas in China is too cold to preserve bulbs naturally. In order to clarify cold-resistance physiology of Curcuma alismatifolia bulbs, and to find out methods to enhance bulbs cold-resistance, various physiological and biochemical indexes of bulbs which were treated with different low temperature stress were detected, and cold-resistance chemicals screening experiment for bulbs were carried out. The results of physiological and biochemical tests showed that Curcuma alismatifolia bulbs began to be stressed at 9 ℃. According to changes of antioxidant enzyme system and osmotic adjustment system, these bulbs mobilized their own stress-resistant system to resist adverse effects at 5 and 1 ℃, respectively, While various stress-resistant related indexes reduced to the lowest point at -3 ℃, which might be beyond the tolerance limit of bulbs. The cold-resistant chemicals screening experiment showed that all chemicals treatments including abscisic acid, salicylic acid, brassinolide, 6-BA, paclobutrazol could enhance cold tolerance of bulbs. According to cell damage degree analysis, 1 mg·L-1 ABA immerged bulbs for 24 h had the best protective effect to bulbs. 0.08 mg·L-1 6-BA treatment also could protect membrane permeability, but the oxidation degree of membrane lipid was very high.

Key words: Curcuma alismatifolia, Chiang Mai Pink, bulb, cold resistance, abscisic acid

中图分类号: