利用分子标记辅助选择改良浙恢7954的稻米品质

doi:10.3969/j.issn.1004-1524.2017.08.01

浙江农业学报 ›› 2017, Vol. 29 ›› Issue (8): 1221-1227.DOI: 10.3969/j.issn.1004-1524.2017.08.01

• 作物科学 • 下一篇

利用分子标记辅助选择改良浙恢7954的稻米品质

牛凯萌^{1, 2}, 徐俊波², 钟亮², 王华², 朱英^{2, *}

1.浙江师范大学化学与生命科学学院,浙江金华 321004；
2.浙江省农业科学院病毒学与生物技术研究所,浙江省植物有害生物防控重点实验室-省部共建国家重点实验室培育基地,浙江杭州 310021

收稿日期:2017-03-09 出版日期:2017-08-20 发布日期:2017-09-06
通讯作者: 朱英,E-mail: yzhuzaas@163.com
作者简介:牛凯萌(1992-),男,山东滨州人,硕士研究生,研究方向为稻米品质改良与分子育种。E-mail: niukaimeng47@163.com
基金资助:
科技部国家重点研发计划(2016YFD0100900); 浙江省自然科学基金委杰出青年基金项目(LR14C02001)

Improving rice quality of ZH7954 by molecular marker-assisted selection

NIU Kaimeng^{1, 2}, XU Junbo², ZHONG Liang², WANG Hua², ZHU Ying^{2, *}

1. College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China;
2. State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2017-03-09 Online:2017-08-20 Published:2017-09-06

摘要/Abstract

摘要： 浙江省第1个获得国家植物新品种保护的浙恢7954是杂交稻育种中综合性状优异的恢复系,但其直链淀粉含量偏高(约25%),食味品质较差。多年来,通过传统育种手段改良该品种的稻米品质,收效甚微。利用Wx基因第1内含子剪切处的SNP位点,设计分子标记,以综合性状优异且直链淀粉含量中低的93-11为供体,借助分子标记辅助选择技术,通过杂交和多代回交,获得了直链淀粉含量中等,食味品质明显提升,而粒型、粒重、精米率等其他综合性状近似于亲本浙恢7954的品系L2和L9,表明成功地改良了浙恢7954的稻米品质。SNP分型结果显示,除Wx位点外,L2和L9在浙恢7954背景下导入的93-11片段均不到7%。这些中间材料的获得,为高产优质的杂交稻亲本及其杂交组合选育提供了良好的基础。

关键词: 浙恢7954, 直链淀粉含量, 蜡质基因, 分子标记辅助育种, SNP

Abstract: Zhehui7954 (ZH7954) is an elite restorer line with high combining ability for three-line indica hybrid rice production. However, the amylose content of ZH7954 was high (25%), which resulted in poor eating and cooking quality. Improvement of rice quality of ZH7954 was hard to achieve by using traditional breeding strategies. In this study, a codominant molecular marker named PCR-AccI was developed based on the G/T SNP at the first intron splicing site of rice Wx gene which mainly determines the amylose biosynthesis in rice endosperm. Here, an elite parental line 93-11 with intermediate AC (17%) and T-type Wx(called Wx^b) were used as donors. Two lines L2 and L9 were obtained with intermediate AC but similar phenotype to ZH7954 by backcross and molecular marker-assisted selection. Both of them had better eating and cooking quality than receptor line ZH7954, while the appearance quality, such as grain shape, grain weight and milled rice rate were almost identical to ZH7954. These results suggested that the quality of ZH7954 was successfully improved through combining the traditional breeding and marker assisted selection. Data from SNP-typing showed that less than 7% of genome from 93-11 was integrated to ZH7954 in both L2 and L9. The combining ability would be further tested and such intermediate lines might be the valuable materials to generate elite restorer line with high yield and good quality in the future.

Key words: ZH7954, amylose content, Wx gene, marker-assisted selection, SNP

中图分类号:

S511

牛凯萌, 徐俊波, 钟亮, 王华, 朱英. 利用分子标记辅助选择改良浙恢7954的稻米品质[J]. 浙江农业学报, 2017, 29(8): 1221-1227.

NIU Kaimeng, XU Junbo, ZHONG Liang, WANG Hua, ZHU Ying. Improving rice quality of ZH7954 by molecular marker-assisted selection[J]. , 2017, 29(8): 1221-1227.

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利用分子标记辅助选择改良浙恢7954的稻米品质

Improving rice quality of ZH7954 by molecular marker-assisted selection

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