基于线粒体COXⅠ基因变异探讨国内外猪种的遗传多样性及系统进化研究

doi:10.3969/j.issn.1004-1524.2017.08.07

浙江农业学报 ›› 2017, Vol. 29 ›› Issue (8): 1271-1280.DOI: 10.3969/j.issn.1004-1524.2017.08.07

基于线粒体COXⅠ基因变异探讨国内外猪种的遗传多样性及系统进化研究

周秀敏, 杨永江, 毕英杰, 任卫合, 张丽^*

西北民族大学生命科学与工程学院,甘肃兰州730030

收稿日期:2017-05-14 出版日期:2017-08-20 发布日期:2017-09-06
通讯作者: 张丽,E-mail: zhangli2008@aliyun.com
作者简介:周秀敏(1996-),女,江苏盐城人,本科生,动物科学专业。E-mail: 1029007641@qq.com
基金资助:
西北民族大学国家级大学生创新创业训练计划项目(201710742088)

Genetic diversity and phylogenetic relationship among domestic and foreign pig breeds based on mtDNA COXⅠ gene

ZHOU Xiumin, YANG Yongjiang, BI Yingjie, REN Weihe, ZHANG Li^*

College of Life Science and Engineering, Northwest University for Nationalities, Lanzhou 730030, China

Received:2017-05-14 Online:2017-08-20 Published:2017-09-06

摘要/Abstract

摘要： 基于mtDNA COXⅠ基因,探讨了6个猪种687个样本(大白猪、长白猪、杜洛克、藏猪、甘肃黑猪和八眉猪)的遗传多样性和各猪种间的亲缘关系。对各猪种样本mtDNACOXⅠ基因构建混合池,并利用直接测序技术获得6个猪种COXⅠ基因的序列,采用MEGA 6.0分析软件基于Kimurk双参数模型应用邻接法构建系统发生树。结果表明,6个猪种mtDNA COXⅠ基因序列共存在21个突变位点,其中8个突变为各群体特有。长白猪与杜洛克猪的多态性较丰富,含有13个相同位点的突变,核苷酸的转换数(si)和颠换数(sv)的比值(R)分别为12和15,序列替换远未达到饱和。而藏猪、黑猪和八眉猪的多态性较贫乏。系统进化树和遗传距离分析显示,6个猪种及野猪先聚为一支而后与同属为偶蹄目的牛、羊聚为一支,3个地方猪种间遗传距离较近。外来猪种引入中国后主要是用作父本以提高生产速度和瘦肉率等,对本地猪种未有母系贡献,线粒体COXⅠ基因可有效区别6个猪种的亲缘关系,在一定程度上为中国地方猪品种的有效保护和合理利用提供理论依据。

关键词: 猪, 线粒体细胞色素C氧化酶Ⅰ, 亚基, 系统地理学

Abstract: Based on the mitochondrial DNA COX Ⅰ (mtDNA COX Ⅰ) gene, the genetic diversity and phylogenetic relationships of 6 pig breeds (Large White pig, Landrace pig, Duroc pig, Zang pig, Gansu black pig and Bamei pig) were studied. The mtDNA COX Ⅰ gene sequences obtained from 6 pig breeds were sequenced and analyzed. Besides, we constructed phylogenetic tree by using MEGA 6.0 analysis software and neighbor joining method based on Kimurk two parameter models. The results showed that there were 21 mutations in sequences of COX Ⅰ (1 545 bp), including 8 group specific mutations. Landrace pig and Duroc pig were abundant in polymorphism (13 same mutation sites), and the ratios (R) of nucleotides’ conversion number (si) and transition number (sv) were 12 and 15, which proved sequence substitutions were far from saturation. While the polymorphisms of Zang pigs, Gansu black pigs and Bamei pigs were poor. The introduced pigs were used as sire, not dam, to improve the growth performance and lean meat percentage of the domestic pig breeds. The mtDNA COX Ⅰ gene can effectively distinguish the relationship between six pig breeds. This study can provide theoretical basis for the effective protection and rational utilization of pig breeds in our country.

Key words: pig, mitochondrial cytochrome C oxidase subunit Ⅰ, phylogeography

中图分类号:

S828

周秀敏, 杨永江, 毕英杰, 任卫合, 张丽. 基于线粒体COXⅠ基因变异探讨国内外猪种的遗传多样性及系统进化研究[J]. 浙江农业学报, 2017, 29(8): 1271-1280.

ZHOU Xiumin, YANG Yongjiang, BI Yingjie, REN Weihe, ZHANG Li. Genetic diversity and phylogenetic relationship among domestic and foreign pig breeds based on mtDNA COXⅠ gene[J]. , 2017, 29(8): 1271-1280.

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基于线粒体COXⅠ基因变异探讨国内外猪种的遗传多样性及系统进化研究

Genetic diversity and phylogenetic relationship among domestic and foreign pig breeds based on mtDNA COXⅠ gene

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