光照环境对大花月季组织培养的影响

doi:10.3969/j.issn.1004-1524.2017.08.10

浙江农业学报 ›› 2017, Vol. 29 ›› Issue (8): 1297-1304.DOI: 10.3969/j.issn.1004-1524.2017.08.10

光照环境对大花月季组织培养的影响

吕永平¹, 陈志¹, 李坤峰², 汪一婷¹, 牟豪杰¹, 陈剑平^{1, *}

1.浙江省农业科学院病毒学与生物技术研究所,浙江杭州 310021；
2.浙江大学农业试验站,浙江杭州 310058

收稿日期:2017-06-05 出版日期:2017-08-20 发布日期:2017-09-06
通讯作者: 陈剑平,E-mail: jpchen2001@126.com
作者简介:吕永平(1977-),男,浙江磐安人,助理研究员,主要从事植物组织培养相关研究。E-mail: lvyongp@163.com
基金资助:
农业部“引进国际先进农业技术”项目(2011-G31,2016-X23)

Influence of light on tissue culture of rose

LYU Yongping¹, CHEN Zhi¹, LI Kunfeng², WANG Yiting¹, MOU Haojie¹, CHEN Jianping^{1, *}

1. Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;
2. Agricultural Experiment Station, Zhejiang University, Hangzhou 310058, China

Received:2017-06-05 Online:2017-08-20 Published:2017-09-06

摘要/Abstract

摘要： 不同色温白光LED和不同LED红、蓝配比光质对大花月季组培苗影响测试结果表明,不同LED红、蓝配比光质下,丛生组培苗的苗高整齐度好于荧光灯和所测试白光LED下的培养材料,而不同色温白光LED和荧光灯下培养材料在所有检测项目中差异均不显著。光周期对大花月季组培苗的影响结果显示,光照时间不低于14 h·d^-1时,所有白光LED下培养材料与荧光灯14和16 h·d^-1下培养材料之间无显著差异。生根试验结果表明,所有处理中大花月季组培苗生根情况无显著差异。综合分析,从生产成本方面考虑,5 000 K白光LED能完全代替荧光灯作为大花月季组织培养的替代光源,光照时间14 h·d^-1即可保证种苗质量。

关键词: 光环境, 大花月季, 组织培养, 发光二极管(LED)

Abstract: The effect of different color temperature white LEDs (Light-emitting diodes) and different combinations of red and blue LEDs on the tissue culture of rose were studied. Results showed that growth of plant materials cultured under the red and blue combination light qualities were significantly better than growth of those cultured under fluorescent and white LEDs in plant height uniform, while the growth of plant cultured under different color temperature white LEDs showed no significant difference from the growth of those cultured under fluorescent lamp. The results of photoperiod on tissue culture of rose indicated that when the LEDs illumination time was not shorter than 14 h·d^-1, there was no significant difference in the plant material cultured under the white LEDs and the fluorescent material at 14 and 16 h·d^-1. The results of rooting induction showed that plant cultured in different light circusmantance had no significant difference in rose rooting indutcion. All the results indicated that 5 000 K white LEDs could completely replace the fluorescent lamp as a rose tissue culture alternative light source, and 14 h·d^-1 photoperiod was enough.

Key words: light, rose, tissue culture, light-emitting diodes (LED)

中图分类号:

S685.12

吕永平, 陈志, 李坤峰, 汪一婷, 牟豪杰, 陈剑平. 光照环境对大花月季组织培养的影响[J]. 浙江农业学报, 2017, 29(8): 1297-1304.

LYU Yongping, CHEN Zhi, LI Kunfeng, WANG Yiting, MOU Haojie, CHEN Jianping. Influence of light on tissue culture of rose[J]. , 2017, 29(8): 1297-1304.

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光照环境对大花月季组织培养的影响

Influence of light on tissue culture of rose

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