黄颡鱼源海豚链球菌分离、鉴定及药敏特性

doi:10.3969/j.issn.1004-1524.2017.06.07

浙江农业学报 ›› 2017, Vol. 29 ›› Issue (6): 903-909.DOI: 10.3969/j.issn.1004-1524.2017.06.07

黄颡鱼源海豚链球菌分离、鉴定及药敏特性

杨移斌^1,2, 胥宁^1,2, 杨秋红^1,2, 刘永涛^1,2, 董靖^1,2, 艾晓辉^1,2,*

1.中国水产科学研究院长江水产研究所,湖北武汉 430223;
2.淡水水产健康养殖湖北省协同创新中心,湖北武汉 430223

收稿日期:2016-12-13 出版日期:2017-06-20 发布日期:2017-09-07
通讯作者: 艾晓辉,E-mail: aixh@yfi.ac.cn
作者简介:杨移斌(1988—),男,江西抚州人,硕士,助理研究员,从事水生动物医学研究。E-mail: yangyb1988@126.com
基金资助:
公益性行业(农业)科研专项(201203085)

Isolation, identification and antibiotic sensitivity of Streptococcus iniae from Pelteobagrus fulvidraco

YANG Yibin^1,2, XU Ning^1,2, YANG Qiuhong^1,2, LIU Yongtao^1,2, DONG Jing^1,2, AI Xiaohui^1,2,*

1. Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China;
2. Hubei Freshwater Aquaculture Collaborative Innovation Center, Wuhan 430223, China

Received:2016-12-13 Online:2017-06-20 Published:2017-09-07

摘要/Abstract

摘要： 为探明福建漳州某养殖场暴发黄颡鱼大量死亡的病因,为该疫病的防控提供参考。从患病黄颡鱼肝、肾及脾分离纯化病原菌,经理化特性测定及16S rRNA 序列分析对其进行鉴定,开展人工感染试验,并利用纸片扩散法进行药敏特性分析。结果显示,分离菌株si222对黄颡鱼的LD₅₀为2.63×10⁴ cfu·g^-1,其理化特性与海豚链球菌(Streptococcus iniae)基本一致,16S rRNA序列与海豚链球菌同源性为100%,综合判定分离菌株为海豚链球菌。菌株si222对苯唑西林、头孢拉定、克林霉素及氯霉素利福平等11种抗生素高度敏感;对青霉素、阿莫西林、磺胺异噁唑及氟苯尼考等6种抗生素耐药。分离菌株si222是黄颡鱼病原菌,养殖时可选用庆大霉素及多西环素等药物进行防控。

关键词: 海豚链球菌, 黄颡鱼, 人工感染, 药敏特性

Abstract: The study was aimed to investigate the cause about the outbreak of numerous deaths of Pelteobagrus fulvidraco at a fishery farm in Zhangzhou, Fujian Province, and to provide reference for the prevention and control of the disease for Pelteobagrus fulvidraco. The pathogenic bacteria were isolated and purified from liver, kidney and spleen of sick Pelteobagrus fulvidraco. And the identification of the strain si222 was conducted, using the biochemical identification and 16S rRNA gene sequence determination. The artificial infection test was performed, and the antimicrobial susceptibility test was conducted by disc diffusion method. The results showed that strain si222 was the pathogens of Pelteobagrus fulvidraco, the LD₅₀ of the isolate was counted to 2.63×10⁴ cfu·g^-1. According to morphological and biochemical characteristics as well as the result of 16S rRNA gene sequence analysis, the isolated strain si222 was Streptococcus iniae. Strain si222 was susceptible to oxacillin, cefadroxil, clindamycin and rifampicin and other 11 kinds of antibiotics. Meanwhile, it showed resistance to penicillin, amoxicillin, sulfisoxazole, florfenicol and other 6 kinds of antibiotics. The isolated strain si222 was the pathogenic bacteria to Pelteobagrus fulvidraco, and the disease can be prevented by administering drugs such as gentamicin and doxycycline in fisheries farming.

Key words: Streptococcus iniae, Pelteobagrus fulvidraco, artificial infection, antibiotic sensitivity

中图分类号:

S941.42

杨移斌, 胥宁, 杨秋红, 刘永涛, 董靖, 艾晓辉. 黄颡鱼源海豚链球菌分离、鉴定及药敏特性[J]. 浙江农业学报, 2017, 29(6): 903-909.

YANG Yibin, XU Ning, YANG Qiuhong, LIU Yongtao, DONG Jing, AI Xiaohui. Isolation, identification and antibiotic sensitivity of Streptococcus iniae from Pelteobagrus fulvidraco[J]. , 2017, 29(6): 903-909.

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黄颡鱼源海豚链球菌分离、鉴定及药敏特性

Isolation, identification and antibiotic sensitivity of Streptococcus iniae from Pelteobagrus fulvidraco

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