浙江农业学报 ›› 2017, Vol. 29 ›› Issue (11): 1796-1799.DOI: 10.3969/j.issn.1004-1524.2017.11.04

• 动物科学 • 上一篇    下一篇

噪声对肉鸭内脏组织热休克蛋白mRNA转录的影响

张扬1, 陈博雯1, 卞友庆2, 王兆山3, 徐琪1, 常国斌1, 陈国宏1, *   

  1. 1.扬州大学 动物科学与技术学院,江苏 扬州 225009;
    2.江苏现代畜牧科技园,江苏 泰州225300;
    3.江苏益客食品有限公司,江苏 宿迁 223800
  • 收稿日期:2017-04-01 出版日期:2017-11-20 发布日期:2017-12-05
  • 通讯作者: 陈国宏,E-mail:ghchen@yzu.edu.cn
  • 作者简介:张扬(1986—),男,山东临沂人,博士,讲师,主要从事动物遗传育种与繁殖研究。E-mail:zyang@yzu.edu.cn
  • 基金资助:
    江苏省农业科技自主创新资金项目[CX(15)1010; CX(14)2070]; 江苏省高校自然科学研究项目(16KJB230002)

Influence of noise on HSPs mRNA transcription in internal organs of meat duck

ZHANG Yang1, CHEN Bowen1, BIAN Youqing2, WANG Zhaoshan3, XU Qi1, CHANG Guobin1, CHEN Guohong1, *   

  1. 1. College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China;
    2. Jiangsu Modern Animal Husbandry Science and Technology Park, Taizhou 225300, China;
    3. Jiangsu Eco Food Co., Ltd, Suqian 223800, China
  • Received:2017-04-01 Online:2017-11-20 Published:2017-12-05

摘要: 为阐明噪声对肉鸭的生理影响,提高肉鸭的动物福利,试验将60只北京鸭随机分成3组,利用噪声发生器进行80 dB(处理组1)和100 dB(处理组2)的噪声应激,对照组不进行应激。应激结束后采集肝脏、脾脏、肺和肾脏组织,采用RT-PCR技术检测各组织中HSP70、HSP90 mRNA转录水平。结果显示:处理组鸭肝脏、脾脏、肺脏和肾脏等4个组织中HSP70、HSP90 mRNA的转录水平极显著(P<0.01)高于对照组,且处理组2的HSP70、HSP90 mRNA的转录水平极显著(P<0.01)高于处理组1。表明急性噪声应激会使得肉鸭内脏组织的HSPs mRNA转录增加。

关键词: 噪声应激, 肉鸭, HSP70, HSP90

Abstract: In order to clarify the physiological effects of noise on meat ducks and improve the animal welfare of meat ducks, 60 ducks were randomly divided into 3 groups. The 80 and 100 dB noise were applied by noise generator to establish stress model in experimental group 1 and 2, respectively. The control group did not apply noise stress. Liver, spleen, lung and kidney tissues were collected after the stress treatment. RT-PCR was used to detect the expression of HSP70 and HSP90 mRNA in each tissue. The results showed that transcription levels of HSP70 and HSP90 mRNA in the liver, spleen, lung and kidney of experimental group 1 and 2 were significantly higher than those in the control group (P<0.01), and the transcription levels of HSP70 and HSP90 mRNA in experimental group 2 were significantly (P<0.01) higher than those in experimental group 1. Therefore, acute noise stress would increase the transcript of HSPs mRNA in the internal organs of meat ducks.

Key words: noise stress, meat duck, HSP70, HSP90

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