酵母硒和硼联合添加对肉鸡免疫器官组织结构及免疫功能的影响

doi:10.3969/j.issn.1004-1524.2017.11.03

浙江农业学报 ›› 2017, Vol. 29 ›› Issue (11): 1783-1795.DOI: 10.3969/j.issn.1004-1524.2017.11.03

酵母硒和硼联合添加对肉鸡免疫器官组织结构及免疫功能的影响

靳二辉, 周金星, 任曼, 胡倩倩, 金光明, 李升和^*

安徽科技学院动物科学学院,安徽凤阳 233100

收稿日期:2017-04-11 出版日期:2017-11-20 发布日期:2017-12-05
通讯作者: 李升和,E-mail:lishhe2005@163.com
作者简介:靳二辉(1976—),男,河南汝州人,博士,副教授,主要从事动物解剖与组织学研究。E-mail:jineh@ahstu.edu.cn
基金资助:
国家自然科学基金(31402154,31502137,31672502); 国家星火计划项目(2015GA710015); 安徽省教育厅重点项目(KJ2017A505,KJ2016A823); 安徽省农业科技成果转化项目(1504032001); 安徽科技学院科研基金重点项目(ZRC2016476,ZRC2013354); 安徽省第七批“115”产业创新团队项目(皖人才[2014] 4号); 安徽科技学院重点建设学科项目(AKZDXK2015A04)

Combined effects of yeast selenium and boron on structure of immune organs and immune function of broilers

JIN Erhui, ZHOU Jinxing, REN Man, Hu Qianqian, JIN Guangming, LI Shenghe^*

College of Animal Science, Anhui Science and Technology University, Fengyang 233100, China

Received:2017-04-11 Online:2017-11-20 Published:2017-12-05

摘要/Abstract

摘要： 为研究酵母硒和硼联用对肉鸡免疫器官发育和免疫功能的影响,试验选用健康1日龄AA肉鸡1 200羽,随机分成对照组和处理Ⅰ、Ⅱ、Ⅲ、Ⅳ组,每组6个重复,每个重复40羽。对照组饲喂基础饲粮,处理Ⅰ~Ⅳ组在基础饲粮中添加0.3 mg·kg^-1的酵母硒和硼(添加量分别为0、5、10、15 mg·kg^-1),试验期42 d。结果显示:1)在21日龄,与对照组相比,处理Ⅱ和Ⅲ组肉鸡法氏囊器官指数,脾小结、动脉周围淋巴鞘和法氏囊小结面积及胸腺皮质厚度均显著(P<0.05)升高;处理Ⅱ组肉鸡血清新城疫病毒(NDV)抗体和干扰素-γ(INF-γ)水平,处理Ⅲ组肉鸡血清白细胞介素-2(IL-2)水平也均显著(P<0.05)升高。2)在42日龄,与对照组相比,处理Ⅱ和Ⅲ组肉鸡胸腺和法氏囊器官指数,脾小结、动脉周围淋巴鞘和法氏囊小结面积,胸腺皮质厚度以及血清NDV抗体和IL-2水平均显著(P<0.05)升高;处理Ⅱ组血清IgG和IFN-γ水平也显著(P<0.05)升高;处理Ⅰ组肉鸡胸腺和法氏囊器官指数及血清IgG水平显著(P<0.05)升高。显微观测可见,处理Ⅱ和Ⅲ组21 d和42 d肉鸡免疫器官组织结构均明显改善,脾脏白髓和红髓结构清晰,胸腺皮质和髓质分界明显,法氏囊小叶数量增多,排列紧密,且处理Ⅰ组肉鸡法氏囊小叶体积也明显增大。结果表明,酵母硒单独补充或与不同剂量的硼联合应用均可在一定程度上促进肉鸡免疫器官的发育,改善其组织结构,增加机体免疫功能,0.3 mg·kg^-1酵母硒与5~10 mg·kg^-1硼联用促进效果更为明显。

关键词: 酵母硒, 硼, 肉鸡, 免疫器官, 组织结构, 免疫功能

Abstract: In order to study the combined effects of yeast selenium and boron on development of immune organ and immune function of broilers, a total of 1 200 one-day-old AA broilers were randomly divided into five groups, the control group and the experimental groups Ⅰ, Ⅱ, Ⅲ and Ⅳ. Each group included 6 pens and 40 birds per pen. The control group was given a basic diet, while experimental groups Ⅰ, Ⅱ, Ⅲ and Ⅳ were given basic diets supplemented with 0.3 mg·kg^-1 yeast selenium and different doses of boron (0, 5, 10, 15 mg·kg^-1) for 42 days, respectively. The results showed as follows: 1) Compared with the control group at 21 days of age, the indexes of bursa of Fabricius and the areas of splenic node, periarterial lymphatic sheath and bursa nodule of Fabricius of broilers in experimental group Ⅱ and Ⅲ were significantly (P<0.05) increased; Serum Newcastle disease virus(NDV) antibody and interferon-γ (INF-γ) levels of broilers in experimental group Ⅱ and the serum interleukin-2 (IL-2) contents of broilers in experimental group Ⅲ were also significantly (P<0.05) increased. 2) Compared with the control group at 42 days of age, the indexes of thymus and bursa of Fabricius, and the levels of serum NDV antibody and IL-2, and the areas of splenic node, periarterial lymphatic sheath and bursa nodule of Fabricius of broilers in experimental group Ⅱ and Ⅲ were significantly (P<0.05) increased; the serum IgG and IFN-γ levels of broilers in experimental group Ⅱ were also significantly (P<0.05) increased; The indexes of thymus and bursa of Fabricius and serum IgG levels of broilers in experimental group Ⅰ were significantly (P<0.05) increased. Microscopic observation also showed that at 21 and 42 days of age, the microstructure of immune organs were improved in broilers of experimental group Ⅱ and Ⅲ, such as the structure of white pulp and red pulp of the spleen were clear, the boundary of thymus cortex and medulla was obvious, the node number of bursa of Fabricius were increased and the arrangement was compacted, and the size of bursa of Fabricius lobule also significantly (P<0.05) increased in experimental group Ⅰ. In conclusion, either the alone supplementation of yeast selenium or combined supplementation of yeast selenium and different doses of boron could promote the development of immune organs, improve microstructure and enhance the immune function of broiler to a certain extent, and combined supplementation of 0.3 mg·kg^-1 yeast selenium and 5-10 mg·kg^-1 boron had more obvious beneficial effects.

Key words: yeas selenium, boron, broiler, immune organ, tissue structure, immune function

中图分类号:

S831

靳二辉, 周金星, 任曼, 胡倩倩, 金光明, 李升和. 酵母硒和硼联合添加对肉鸡免疫器官组织结构及免疫功能的影响[J]. 浙江农业学报, 2017, 29(11): 1783-1795.

JIN Erhui, ZHOU Jinxing, REN Man, Hu Qianqian, JIN Guangming, LI Shenghe. Combined effects of yeast selenium and boron on structure of immune organs and immune function of broilers[J]. , 2017, 29(11): 1783-1795.

参考文献

[1] 张江. 有机微量元素对家畜免疫、健康、生产和繁殖的影响[J]. 国外畜牧学-猪与禽,2015(2):73-74.
ZHANG J. Effects of organic trace elements on immunity, health, production and reproduction of domestic animals[J]. Animal Science Abroad : Pigs and Poultry , 2015(2):73-74. (in Chinese)
[2] MAGGINI S, WINTERGERST E S, BEVERIDGE S, et al. Selected vitamins and trace elements support immune function by strengthening epithelial barriers and cellular and humoral immune responses[J]. British Journal of Nutrition , 2007, 98(Suppl.1):S29-S35.
[3] BENTON D. Selenium intake, mood and other aspects of psychological functioning[J]. Nutritional Neuroscience , 2013, 5(6): 363-374.
[4] 陈国顺,吴劲峰,李冲. 不同来源硒对肉鸡生产性能、免疫功能和抗氧化性能的影响[J]. 中国家禽,2014,36(11):22-25.
CHEN G S, WU J F, LI C. Effect of different selenium sources on production performance, immune function and antioxidant capacity of broilers[J]. China Poultry , 2014, 36(11):22-25. (in Chinese with English abstract)
[5] CHEN G, WU J, LI C. Effect of different selenium sources on production performance and biochemical parameters of broilers[J]. Journal of Animal Physiology and Animal Nutrition , 2014, 98(4):747-754.
[6] 陈忠法,俞信光,韩泽建. 不同硒源对肉仔鸡生长性能和肉质的影响[J]. 浙江农业学报,2003, 15(4):250-254.
CHEN Z F, YU X G, HAN Z J. Effects of different selenium resources on growth performance and meat quality in broiler[J]. Acta Agriculturae Zhejiangensis , 2003, 15(4):250-254. (in Chinese with English abstract)
[7] 李晓丽,何万领,位治国,等. 酵母硒对蛋鸡生产性能、血清生化指标及常规蛋品质的影响[J]. 中国兽医学报,2015,35(11):1838-1839.
LI X L, HE W L, WEI Z G, et al. Effects of yeast-Se on production performance, serum biochemical parameters and egg quality of laying hens[J]. Chinese Journal of Veterinary Science , 2015, 35(11):1838-1839. (in Chinese with English abstract)
[8] 罗霄,方俊,刘刚,等. 日粮中添加酵母硒对断奶仔猪生长性能及抗氧化性能的影响[J]. 饲料工业,2015,36(6):6-10.
LUO X, FANG J, LIU G, et al. Effect of dietary yeast selenium on the growth performance and antioxidant properties of Duroc×Landrace×Yorkshire weaned piglets[J]. Feed Industry , 2015, 36(6):6-10. (in Chinese in Chinese with English abstract)
[9] 于枫,徐海滨,陈凤梅,等. 酵母硒与酵母多糖联合应用对奶牛抗热应激的作用[J]. 动物医学进展,2016,37(1):34-37.
YU F, XU H B, CHEN F M, et al. Effect of selenium yeast combined with yeast polysaccharide on anti-heat stress in dairy cows[J]. Progress in Veterinary Medicine , 2016, 37(1):34-37. (in Chinese with English abstract)
[10] 王巧莉,王宝维,范永存,等. 酵母硒对肉鹅免疫和抗氧化指标的影响[J]. 动物营养学报,2009,21(3):398-404.
WANG Q L, WANG B W, FAN Y C, et al. Effects of selenium yeast on immune and anti-oxidation indices of geese[J]. Acta Zoonutrimenta Sinica , 2009, 21(3):398-404. (in Chinese with English abstract)
[11] MEHMET B, KAMIL K, ABDULLAH U, et al. Effects of boron supplementation to diets deficient in calcium and phosphorus on performance with some serum, bone and fecal characteristics of broiler chickens[J]. Asian Australasian Journal of Animal Sciences , 2012, 25(2):248-255.
[12] MIZRAK C, YENICE E, CAN M, et al. Effects of dietary boron on performance, egg production, egg quality and some bone parameters in layer hens[J]. South African Journal of Animal Science , 2010, 40(3):257-264.
[13] BAKKEN N A, HUNT C D. Dietary boron decreases peak pancreatic in situ insulin release in chicks and plasma insulin concentrations in rats regardless of vitamin d or magnesium status[J]. Journal of Nutrition , 2003, 133(11):3577-3583.
[14] NAGHII M R, MOFID M, ASGARI A R, et al. Comparative effects of daily and weekly boron supplementation on plasma steroid hormones and proinflammatory cytokines[J]. Journal of Trace Elements in Medicine and Biology , 2011, 25(1):54-58.
[15] STANLEY T R, SMITH G J, HOFFMAN D J, et al. Effects of boron and selenium on mallard reproduction and duckling growth and survival[J]. Environmental Toxicology and Chemistry , 1996, 15(7):1124- 1132.
[16] 王宝维,王娜,葛文华,等. 不同硒源对鹅早期生产性能、屠宰性能、肉品质、肌肉常规养分、免疫与抗氧化功能的影响[J]. 中国农业科学,2011,44(14):3016-3026.
WANG B W, WANG N, GE W H, et al. Effects of different selenium sources on production performance, slaughter performance, meat quality, immune and antioxidant in the early goose[J]. Scientia Agricultura Sinica , 2011, 44(14):3016-3026. (in Chinese with English abstract)
[17] JIN E H, GU Y F, WANG J, et al. Effect of supplementation of drinking water with different levels of boron on the performance and immune organ parameters of broilers[J]. Italian Journal of Animal Science , 2014, 13(2):24-134.
[18] RIVAS A L, FABRICANT T. Indication of immunodepression in chicken infected various strain of Marek’s disease virus[J]. Avian Diseases , 1988, 32(1):1-8.
[19] 刘扬,冯琳,赵波,等. 豆粕替代部分鱼粉饲料中添加核苷酸和有机硒对幼建鲤生长和消化吸收功能的影响[J]. 四川农业大学学报,2012,30(2):238-242.
LIU Y, FENG L, ZHAO B, et al. Effects of nucleotides and organic selenium supplementation on growth and digestive ability of juvenile jian carp ( Cyprinus carpio var. jian) in diets with partial substitution of fish meal by soybean meal[J]. Journal of Sichuan Agricultural University , 2012, 30(2):238-242. (in Chinese with English abstract)
[20] 刘红露,范磊,戴茜茜,等. 面包酵母硒与酪酸梭菌制剂对肉鸡生长、抗氧化和肠道菌群的影响[J]. 浙江农业学报,2015,27(9):1529-1534.
LIU H L,FAN L, DAI X X, et al. Effects of Saccharomyces cerevisiae selenium and Clostridium butyricum additive on growth, antioxidant ability and intestinal microflora[J]. Acta Agriculturae Zhejiangensis , 2015, 27(9):1529-1534. (in Chinese with English abstract)
[21] 王珏,李升和,金光明,等. 饮水硼对动物空肠发育的影响[J]. 微量元素与健康研究,2007,24(2):1-4.
WANG J, LI S H, JIN G M, et al. Effect of drinking-boron on jejunum development of animal[J]. Studies of Trace Elements and Health , 2007, 24(2):1-4. (in Chinese with English abstract)
[22] HOFFMAN D J, SANDERSON C J, LECAPTAIN L J, et al. Interactive effects of arsenate, selenium, and dietary protein on survival, growth, and physiology in mallard ducklings[J]. Archives of Environmental Contamination and Toxicology , 1991, 20(2):163-171.
[23] XU P, ZENG L X, SCHRAUZER G N, et al. Selenium, boron, and germanium deficiency in the etiology of Kashin-Beck disease[J]. Biological Trace Element Research , 2000, 77(3):193-197.
[24] 邓俊良,陈俊杰,左之才,等. 硼对铜中毒肉用乌骨鸡肝铜含量及肝功能的影响[J]. 中国兽医学报,2009,29(5):657-660.
DENG J L, CHEN J J, ZUO Z C, et al. Effects of copper content in liver and liver function indexes of black bone broiler by increasing boron anti copper poisoning[J]. Chinese Journal of Veterinary Science , 2009, 29(5):657-660. (in Chinese with English abstract)
[25] 秦俊法. 硼的生物必需性及人体健康效应[J]. 广东微量元素科学,1999(9):1-14.
QIN J F. Biological essentiality of boron and its health effects in humans[J]. Guangdong Trace Elements Science , 1999(9):1-14. (in Chinese with English abstract)
[26] 王海娜. 有机硒对小鼠生殖生理功能影响的研究 [D]. 贵阳:贵州大学,2009.
WANG H N. The effect of organic selenium on reproductive physiology function in mouse [D]. Guiyang: Guizhou University, 2009. (in Chinese with English abstract)
[27] 陈大伟,陆俊贤,蒲俊华,等. 铅对蛋鸡肾脏的氧化损伤及酵母硒的保护效应研究[J]. 中国家禽,2014,36(21):27-31.
CHEN D W, LU J X, PU J H, et al. Oxidative damage caused by lead on kidney of layers and protective effect of yeast selenium[J]. China Poultry , 2014, 36(21):27-31. (in Chinese with English abstract)
[28] 王珏,黄亚军,梁林,等. 中药富硒酵母对动物中枢免疫器官发育的影响[J]. 微量元素与健康研究,2009,26(2):8-11.
WANG J, HUANG Y J, LIANG L, et al. Effect of Se-enriched yeast cultured in Chinese medicine on development of central immune organ in animal[J]. Studies of Trace Elements and Health , 2009, 26(2):8-11. (in Chinese with English abstract)
[29] 李升和,范光丽,彭克美,等. 不同硼水平对固始鸡脾发育的影响[J]. 中国兽医科学,2008,38(1):63-67.
LI S H, FAN G L, PENG K M, et al. Effects of different doses of boron on development of spleen in Gushi chicken[J]. Chinese Veterinary Science , 2008, 38(1):63-67. (in Chinese with English abstract)
[30] 李升和,范光丽,顾有方,等. 饮水硼对固始鸡中枢免疫器官发育的影响[J]. 西北农林科技大学学报(自然科学版),2009,37(2):52-58.
LI S H, FAN G L, GU Y F, et al. Effect of drinking boron supplemented on the development of center immune organs in Gushi chickens[J]. Journal of Northwest A & F University ( Natural Science Edition ), 2009, 37(2):52-58. (in Chinese with English abstract)
[31] 李升和,彭克美,马云初,等. 饮水硼对大鼠舌和胃黏膜组织结构的影响[J]. 中国兽医学报,2008,8(11):1329-1334.
LI S H, PENG K M, MA Y C, et al. Effect of drinking boron on structure of lingual mucous membrane and gastricmucosa in rats[J]. Chinese Journal of Veterinary Science , 2008, 8(11):1329-1334. (in Chinese with English abstract)
[32] NIELSEN F H, MEACHAM S L. Growing evidence for human health benefits of boron[J]. Journal of Evidence-Based Complementary and Alternative Medicine , 2011, 16(3):169-180.
[33] HU Q, LI S, QIAO E, et al. Effects of boron on structure and antioxidative activities of spleen in rats[J]. Biological Trace Element Research , 2014, 158(1):73-80.
[34] WINTER H, VAN DEN ENGEL N K, RÅTTINGER D, et al. Therapeutic T cells induce tumor-directed chemotaxis of innate immune cells through tumor-specific secretion of chemokines and stimulation of B16BL6 melanoma to secrete chemokines[J]. Journal of Translational Medicine , 2007, 5:56.
[35] GONZALES J R, GRÖGER S, BOEDEKER R H, et al. Expression and secretion levels of Th1 and Th2 cytokines in patients with aggressive periodontitis[J]. Clinical Oral Investigation , 2012, 16(5):1463-1473.
[36] MONTGOMERY J B, WICHTEL J J, WICHTEL M G, et al. Effects of selenium source on measures of selenium status and immune function in horses[J]. Journal of Equine Veterinary Science , 2012, 32(6):352-359
[37] ARMSTRONG T A, SPEARS J W. Effect of boron supplementation of pig diets on the production of tumor necrosis factor-alpha and interferon-gamma[J]. Journal of Animal Science , 2003, 81(10):2552-2561.
[38] 董栅杉. 饮水硼对雌性大鼠血液生理指标的影响[J]. 天津师范大学学报(自然科学版),2015,35(3):130-133.
DONG S S. Effect of drinking boron on blood physiological parameters of female rats[J]. Journal of Tianjin Normal University ( Natural Science Edition ), 2015, 35(3):130-133. (in Chinese with English abstract)
[39] FAIRBROTHER A, FIX M, O’HARA T, et al. Impairment of growth and immune function of avocet chicks from sites with elevated selenium, arsenic, and boron[J]. Journal of Wildlife Diseases , 2015, 30(2):222-233.
[40] 孙春阳,邢焕,栾素军,等. 葡萄糖氧化酶和酵母硒复合添加剂对肉鸡生长性能、抗氧化性能及肉品质的影响[J]. 动物营养学报,2014,26(9):2789-2796.
SUN C Y, XING H, LUAN S J, et al. Effects of compound additive with glucose oxidase and selenium yeast on growth performance, antioxidant capacity and meat quality of broilers[J]. Acta Zoonutrimenta Sinica , 2014, 26(9):2789-2796. (in Chinese with English abstract)
[41] TURKEZ H, GEYIKOGLU F. Boric acid: a potential chemoprotective agent against aflatoxin b1 toxicity in human blood[J]. Cytotechnology , 2010, 62(2):157-165.

酵母硒和硼联合添加对肉鸡免疫器官组织结构及免疫功能的影响

Combined effects of yeast selenium and boron on structure of immune organs and immune function of broilers

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