高温胁迫对小黄鱼肝脏组织结构和细胞凋亡的影响

doi:10.3969/j.issn.1004-1524.20230230

浙江农业学报 ›› 2024, Vol. 36 ›› Issue (1): 58-66.DOI: 10.3969/j.issn.1004-1524.20230230

高温胁迫对小黄鱼肝脏组织结构和细胞凋亡的影响

何雨¹(), 刘峰²^,^*(), 张天乐³, 楼宝², 魏福亮², 叶挺²

1.湖州师范学院生命科学学院,浙江湖州 313002
2.浙江省农业科学院水生生物研究所,农产品质量安全危害因子与风险防控国家重点实验室,浙江杭州 310021
3.中国计量大学生命科学学院,浙江杭州 310018

收稿日期:2023-02-25 出版日期:2024-01-25 发布日期:2024-02-18
作者简介:何雨(1996—),女,四川内江人,硕士研究生,从事鱼类遗传育种研究。E-mail: 2412069997@qq.com
通讯作者: * 刘峰,E-mail: lengfeng0210@126.com
基金资助:
国家自然科学基金(32102765);浙江省重点研发计划(2021C02055)

Histological study on the effect of high temperature stress on liver tissue in Larimichthys polyactis

HE Yu¹(), LIU Feng²^,^*(), ZHANG Tianle³, LOU Bao², WEI Fuliang², YE Ting²

1. College of Life Sciences, Huzhou Normal University, Huzhou 313002, Zhejiang, China
2. State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Hydrobiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
3. College of Life Sciences, China Jiliang University, Hangzhou 310018, China

Received:2023-02-25 Online:2024-01-25 Published:2024-02-18

摘要/Abstract

摘要：

为探究高温胁迫对小黄鱼(Larimichthys polyactis)肝脏组织结构和细胞凋亡的影响,研究通过人工控温,按照2 ℃·h^-1的升温速率将水温从自然水温15 ℃升高至31 ℃(实验组)并维持此温度至96 h,同时设置15 ℃的常温对照组。分别于处理组水温达到31 ℃后的0、6、12、24、48、72、96 h时,同时采集处理组和对照组肝脏组织,进行组织切片观察肝脏组织的组织学变化,采用脱氧核糖核苷酸末端转移酶介导的缺口末端标记(TUNEL)法检测肝脏组织细胞凋亡情况。结果显示,31 ℃处理0 h时肝脏组织显微结构和超微结构与对照组相类似,无显著变化。但是,随着高温处理时间的延长,肝脏组织损伤逐渐加重,96 h后肝脏呈现严重损伤,表现为水肿、细胞核消失甚至溶解和肝组织局部坏死,以及肝细胞原有结构破坏等现象。此外,随着高温胁迫的持续,肝脏细胞凋亡现象逐渐加重。结果表明,高温胁迫对小黄鱼的肝脏组织造成一定程度的损害,并诱导大量细胞凋亡。研究结果明确了高温胁迫对小黄鱼肝脏组织的影响作用,对于小黄鱼养殖管理避免肝脏疾病发生具有重要的指导意义。

关键词: 小黄鱼, 高温胁迫, 肝脏, 组织结构, 细胞凋亡

Abstract:

In order to explore the effect of high temperature stress on the liver tissue structure and apoptosis of Larimichthys polyactis, the rearing temperature was raised from 15 ℃ to 31 ℃ by altering the water temperature in the tank at a constant rate of 2 ℃·h^-1, and maintained to 96 h, recorded as the test group. At the same time, a control group rearing under the temperature of 15 ℃ was set. At the 0, 6, 12, 24, 48, 72, 96 h after the rearing temperature of the test group reached 31 ℃, the liver tissue of the test and control group were collected to investigate the histological changes and apoptosis of the liver tissues through the methods of tissue sections and terminal-deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL), respectively. The results showed that the liver morphology of microstructure and ultrastructure were similar to those of the control group after 0 h treatment at 31 ℃, and there was no significant change. However, with the extension of high temperature treatment time, liver tissue damage gradually worsened. After 96 h of treatment, the liver showed serious damage, including edema, disappearance or even dissolution of nuclei, local necrosis of liver tissue, and destruction of the original structure of liver cells. In addition, with the continuation of high temperature stress, the phenomenon of liver cell apoptosis gradually aggravated. The results showed that high temperature stress caused a certain degree of damage to the liver tissue of L. polyactis and induced a large number of liver cell apoptosis. The results of the present study clarified the effect of high temperature stress on the liver tissue of L. polyactis, which is of great significance for the rearing management of L. polyactis to avoid the occurrence of liver disease.

Key words: Larimichthys polyactis, high-temperature stress, liver, organization structure, apoptosis

中图分类号:

S965.323

何雨, 刘峰, 张天乐, 楼宝, 魏福亮, 叶挺. 高温胁迫对小黄鱼肝脏组织结构和细胞凋亡的影响[J]. 浙江农业学报, 2024, 36(1): 58-66.

HE Yu, LIU Feng, ZHANG Tianle, LOU Bao, WEI Fuliang, YE Ting. Histological study on the effect of high temperature stress on liver tissue in Larimichthys polyactis[J]. Acta Agriculturae Zhejiangensis, 2024, 36(1): 58-66.

图/表 3

图1 高温胁迫后小黄鱼肝脏显微结构变化(H.E 染色,400×) A,对照;B,高温处理0 h;C,高温处理6 h;D,高温处理12 h;E,高温处理24 h;F,高温处理48 h;G,高温处理72 h;H,高温处理96 h。CV,中央静脉;HC,肝细胞;BC,血细胞;VD,空泡;AB,淤血;N,细胞核;Oe,水肿;H,结构破坏。

Fig.1 Histology of Larimichthys polyactis liver after high temperature stress (H.E, 400×) A, Control; B, 0 h high temperature stress; C, 6 h high temperature stress; D, 12 h high temperature stress; E, 24 h high temperature stress; F, 48 h high temperature stress; G, 72 h high temperature stress; H, 96 h high temperature stress. CV, Central veins; HC, Hepatocyte; BC, Blood corpuscle; VD, Vacuolation; AB, Gore; N, Nucleus; Oe, Oedema; H, Structural damage.

图2 高温胁迫后小黄鱼肝脏超微结构变化(4 000×) A,对照;B,高温处理0 h;C,高温处理6 h;D,高温处理12 h;E,高温处理24 h;F,高温处理48 h;G,高温处理72 h;H, 高温处理96 h。RER,粗面内质网;M,线粒体;F,脂滴;SER,滑面内质网;L,脂褐质;PL,初级溶酶体;SL,次级溶酶体。

Fig.2 Ultrastructure of Larimichthys polyactis liver under high temperature stress(4 000×) A, Control; B, 0 h high temperature stress; C, 6 h high temperature stress; D, 12 h high temperature stress; E, 24 h high temperature stress; F, 48 h high temperature stress; G, 72 h high temperature stress; H, 96 h high temperature stress. RER, Rough endoplasmic reticulum; M, Mitochondrion; F, Lipid droplet; SER, Smooth endoplasmic reticulum; L,Lipofuscin; PL,Primary lysosome; SL, Secondary lysosome.

图3 高温胁迫下小黄鱼肝脏细胞凋亡观察(400×) A,对照;B,高温处理0 h;C,高温处理6 h;D,高温处理12 h;E,高温处理24 h;F,高温处理48 h;G,高温处理72 h;H, 高温处理96 h。箭头代表凋亡细胞(棕褐色)。

Fig.3 Observation of apoptosis of Larimichthys polyactis liver cells under high temperature stress (400×) A, Control; B, 0 h high temperature stress; C, 6 h high temperature stress; D, 12 h high temperature stress; E, 24 h high temperature stress; F, 48 h high temperature stress; G, 72 h high temperature stress; H, 96 h high temperature stress. Arrows in figure represent apoptotic cells (Brown).

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高温胁迫对小黄鱼肝脏组织结构和细胞凋亡的影响

Histological study on the effect of high temperature stress on liver tissue in Larimichthys polyactis

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