羊栖菜优良品系对高温的耐受性比较

doi:10.3969/j.issn.1004-1524.20221808

浙江农业学报 ›› 2024, Vol. 36 ›› Issue (1): 148-155.DOI: 10.3969/j.issn.1004-1524.20221808

羊栖菜优良品系对高温的耐受性比较

左晓洁(), 吴明江, 罗琳, 马增岭, 庞观凤, 陈斌斌()

a.温州大学生命与环境科学学院,浙江温州 325035
b.温州大学浙江省水环境与海洋生物资源保护重点实验室,浙江温州 325035

收稿日期:2022-12-22 出版日期:2024-01-25 发布日期:2024-02-18
作者简介:左晓洁(1998—),女,河南开封人,硕士研究生,主要从事藻类生理方面的研究。E-mail:153703419@qq.com
通讯作者: * 陈斌斌,E-mail: binbch@163.com
基金资助:
国家重点研发计划(2018YFD0901500);国家自然科学基金(41706147);国家自然科学基金(41876124)

Comparison of tolerance to high temperature of excellent strains of Sargassum fusiforme

ZUO Xiaojie(), WU Mingjiang, LUO Lin, MA Zengling, PANG Guanfeng, CHEN Binbin()

a. College of Life and Environmental Science, Wenzhou University,Wenzhou 325035, Zhejiang, China
b. Zhejiang Provincial Key Lab for Subtropical Water Environment and Marine Biological Resources Protection, Wenzhou University,Wenzhou 325035, Zhejiang, China

Received:2022-12-22 Online:2024-01-25 Published:2024-02-18

摘要/Abstract

摘要：

以3种羊栖菜优良品系和野生型羊栖菜为材料,在25 ℃培养,比较高温条件下羊栖菜的生长状况,为耐高温品系的筛选提供数据支持。结果表明,长时间高温培养可降低各品系羊栖菜的光合性能,抑制其硝态氮的吸收与同化相关酶的活性,降低可溶性蛋白和可溶性碳水化合物的含量,增强其暗呼吸速率,最终抑制藻体生长。在高温条件下,相比其他品系,梭形气囊型羊栖菜的最大电子传递速率和饱和光强放氧速率最高,过氧化物酶活性最高且显著增加,丙二醛含量并没有显著增加。总体上梭形气囊型羊栖菜受高温的影响较小,具有较强的耐受性。在实际养殖生产中可以推广梭形气囊型羊栖菜,研究结果可为后续的品种改良提供依据。

关键词: 羊栖菜, 种苗繁育, 高温胁迫, 光合作用, 海藻养殖

Abstract:

Three excellent strains of Sargassum fusiforme and wild-type S. fusiforme were cultured at 25 ℃ to compare the growth status of S. fusiforme under high temperature conditions, and to provided data support for the screening of high temperature resistance strains. The results showed that long-term high temperature culture could reduce the photosynthetic performance of each strain of S. fusiforme, inhibit the activity of nitrate nitrogen absorption and assimilation related enzymes, reduce the content of soluble protein and soluble carbohydrate, enhance its dark respiration rate, and ultimately inhibit the growth of algae. Under high temperature condition, compared with other strains, the maximum electron transfer rate and saturated light intensity oxygen release rate of spindle vesicle type S. fusiforme were the highest, the peroxidase activity was the highest and increased significantly, while its malondialdehyde content did not increase significantly. In general, spindle vesicle type of S. fusiforme was less affected by high temperature and had strong tolerance. Spindle vesicle type of S. fusiforme could be popularized in actual breeding production, and the research results could provide a basis for subsequent variety improvement.

Key words: Sargassum fusiforme, seedlings breeding, high temperature stress, photosynthesis, seaweed aquaculture

中图分类号:

S968.425

左晓洁, 吴明江, 罗琳, 马增岭, 庞观凤, 陈斌斌. 羊栖菜优良品系对高温的耐受性比较[J]. 浙江农业学报, 2024, 36(1): 148-155.

ZUO Xiaojie, WU Mingjiang, LUO Lin, MA Zengling, PANG Guanfeng, CHEN Binbin. Comparison of tolerance to high temperature of excellent strains of Sargassum fusiforme[J]. Acta Agriculturae Zhejiangensis, 2024, 36(1): 148-155.

图/表 5

图1 20 ℃和25 ℃下羊栖菜各品系的形态和相对生长速率 A为形态变化;B和C分别为20 ℃和25 ℃条件下羊栖菜的相对生长速率。图中WT为野生型羊栖菜,SF-1为圆气囊型羊栖菜,SF-2为细长气囊型羊栖菜,SF-3为梭形气囊型羊栖菜。下同。

Fig.1 Morphological and relative growth rate of different Sargassum fusiforme strains under 20 ℃ and 25 ℃ A is the morphological change, B and C are relative growth rates of S. fusiforme strains cultured at 20 ℃ and 25 ℃, respectively. WT is wild type of S. fusiforme, SF-1 is round vesicle type of S. fusiforme, SF-2 is slender vesicle type of S. fusiforme, and SF-3 is spindle vesicle type of S. fusiforme. The same as below.

图2 20 ℃和25 ℃下羊栖菜各品系的光合特性柱上无相同字母表示不同处理间差异显著(P <0.05)。*,表示同一温度处理下羊栖菜各品系在各指标上的差异(*,P <0.05;**,P <0.01)。下同。

Fig.2 Photosynthesis characteristics of different Sargassum fusiforme strains under 20 ℃ and 25 ℃ Data on the bars marked without the same letter indicated significant differences at P<0.05. *, Represents the differences in various indicators of differen S. fusiforme strains under the same temperature treatment (*, P <0.05; **, P <0.01). The same as below.

图3 20 ℃和25 ℃下羊栖菜各品系的$\mathrm{NO}_{3}^{-}$吸收与利用特性

Fig.3 Nitrate absorption and utilization characteristics of S. fusiforme strains under 20 ℃ and 25 ℃

图4 20 ℃和25 ℃下羊栖菜各品系的可溶性蛋白与可溶性碳水化合物含量

Fig.4 Soluble protein and soluble carbohydrate content of different S. fusiforme strains under 20 ℃ and 25 ℃

图5 20 ℃和25 ℃下羊栖菜各品系的超氧化物歧化酶活性、过氧化物酶活性与丙二醛含量

Fig.5 Superoxide dismutase activity, peroxidase activity and malondialdehyde content of different S. fusiforme strains under 20 ℃ and 25 ℃

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羊栖菜优良品系对高温的耐受性比较

Comparison of tolerance to high temperature of excellent strains of Sargassum fusiforme

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