菜心耐热性评价及酶促抗氧化系统对高温胁迫的响应

doi:10.3969/j.issn.1004-1524.2020.01.09

浙江农业学报 ›› 2020, Vol. 32 ›› Issue (1): 72-79.DOI: 10.3969/j.issn.1004-1524.2020.01.09

菜心耐热性评价及酶促抗氧化系统对高温胁迫的响应

庞强强, 周曼, 孙晓东, 张文, 蔡兴来^*

海南省农业科学院蔬菜研究所,海南省蔬菜生物学重点实验室,海南省瓜菜育种工程技术研究中心,海南海口571100

收稿日期:2019-05-09 出版日期:2020-01-25 发布日期:2020-03-11
通讯作者: ^*蔡兴来,E-mail:13307531052@163.com
作者简介:庞强强(1987—),男,安徽宿州人,硕士研究生,研究实习员,主要从事蔬菜栽培与育种。E-mail:qiang2017@sina.cn
基金资助:
2017年海南省重大科技计划(ZDKJ2017001); 海南省重点研发计划(ZDYF2019066)

Evaluation of heat tolerance and response of enzymatic antioxidant system to heat stress in Brassica parachinensis L.

PANG Qiangqiang, ZHOU Man, SUN Xiaodong, ZHANG Wen, CAI Xinglai^*

Vegetable Research Institute for Hainan Provincial Academy of Agricultural Sciences, Hainan Provincial Key Laboratory for Vegetable and Biology, Hainan Provincial Engineering Research Center for Melon and Vegetable Breeding, Haikou 571100, China

Received:2019-05-09 Online:2020-01-25 Published:2020-03-11

摘要/Abstract

摘要： 为鉴定不同菜心品种的耐热性,以热害指数为评价指标对19份材料进行耐热性评价,并在此基础上研究了酶促抗氧化系统对人工模拟高温胁迫及恢复的响应。结果表明:通过热害指数分析,筛选出耐热品种5个、中等耐热品种11个、不耐热品种3个。对耐热品种CX10、中等耐热品种CX17和不耐热品种CX13进行人工模拟高温处理发现,37 ℃高温胁迫时菜心叶片的电导率、MDA含量显著升高,SOD、POD和CAT的活性,以及Mn-SOD、POD和CAT基因表达量显著增加。从增加幅度来看,耐热材料中的电导率、MDA含量增幅明显低于不耐热材料,SOD、POD和CAT的活性,以及Mn-SOD、POD和CAT基因表达量则以耐热材料中增幅最大。在胁迫恢复3 d时,与胁迫时相比,所有指标均呈下降趋势,但仍高于对照。以上结果说明,高温胁迫影响了菜心酶促抗氧化系统的正常代谢,即使高温解除后也不能恢复至正常状态,但耐热材料可通过调节自身的酶促系统减轻胁迫带来的伤害。

关键词: 菜心, 耐热性, 酶促抗氧化系统, 高温胁迫

Abstract: In order to identify the heat tolerance of different varieties of Brassica parachinensis L., heat damage index was used as an evaluation index to evaluate the heat tolerance of 19 materials. On this basis, the response of enzymatic antioxidant system to simulated high temperature stress was studied. The results showed that through the analysis of heat damage index, 5 heat-resistant varieties, 11 medium heat-resistant varieties and 3 heat-sensitive varieties were screened out. The heat-resistant variety CX10, medium-heat-resistant variety CX17 and heat-sensitive variety CX13 under high temperature treatment showed that conductivity, MDA contents, activities of SOD, POD and CAT, expression levels of the related genes Mn-SOD, POD and CAT were increased compared with the control. The increase of conductivity and MDA content in heat-resistant varieties was significantly lower than that in heat-sensitive varieties. SOD, POD and CAT activity, as well as the expression of Mn-SOD, POD and CAT genes in the heat-resistant varieties, increased largest. After 3 days' recovery, compared with the treatment under stress, all indicators showed a downward trend, but higher than the control. These results indicated that high temperature stress affected the normal metabolism of enzymatic antioxidant system in Brassica parachinensis L. Even after high temperature was relieved, it could not return to the normal state. However, heat-resistant varieties could alleviate the damage caused by stress by regulating their protective system.

Key words: Brassica parachinensis L., heat tolerance, enzymatic antioxidant system, heat stress

中图分类号:

S634.5

庞强强, 周曼, 孙晓东, 张文, 蔡兴来. 菜心耐热性评价及酶促抗氧化系统对高温胁迫的响应[J]. 浙江农业学报, 2020, 32(1): 72-79.

PANG Qiangqiang, ZHOU Man, SUN Xiaodong, ZHANG Wen, CAI Xinglai. Evaluation of heat tolerance and response of enzymatic antioxidant system to heat stress in Brassica parachinensis L.[J]. , 2020, 32(1): 72-79.

参考文献

[1] 肖国举, 张强, 王静. 全球气候变化对农业生态系统的影响研究进展[J]. 应用生态学报, 2007, 18(8): 1877-1885.
XIAO G J, ZHANG Q, WANG J.Impact of global climate change on agro-ecosystem: a review[J]. Chinese Journal of Applied Ecology, 2007, 18(8): 1877-1885.(in Chinese with English abstract)
[2] 曾小玲, 方淑桂, 朱朝辉, 等. 不同基因型菜心游离小孢子培养和植株再生[J]. 热带作物学报, 2014, 35(12): 2397-2402.
ZENG X L, FANG S G, ZHU C H, et al.Isolated microspore culture and plant regeneration in flowering Chinese cabbage[J]. Chinese Journal of Tropical Crops, 2014, 35(12): 2397-2402.(in Chinese with English abstract)
[3] 张振贤. 蔬菜栽培学[M]. 北京: 中国农业大学出版社, 2003.
[4] 李光光, 张财顺, 郑岩松. 不同菜心品种耐热性鉴定指标的筛选[J]. 北方园艺, 2012(18): 1-5.
LI G G, ZHANG C S, ZHENG Y S.Screening of assessment indexes on heat tolerance in different Chinese flowering cabbage cultivars[J]. Northern Horticulture, 2012(18): 1-5.(in Chinese with English abstract)
[5] 曹毅, 李春梅, 邓燏, 等. 不同菜心品种耐热性研究[J]. 西南师范大学学报(自然科学版), 2010, 35(5): 128-131.
CAO Y, LI C M, DENG Y, et al.Study on heat tolerance of different Brassica campestris L.ssp. chinensis var. utilis Tsen et Lee varieties[J]. Journal of Southwest China Normal University(Natural Science Edition), 2010, 35(5): 128-131.(in Chinese with English abstract)
[6] QU A L, DING Y F, JIANG Q, et al.Molecular mechanisms of the plant heat stress response[J]. Biochemical and Biophysical Research Communications, 2013, 432(2): 203-207.
[7] 杨妍妍, 杨建平, 王清华, 等. 高温胁迫对大白菜超微弱发光及其他生理指标的影响[J]. 西北农业学报, 2006, 15(5): 218-221.
YANG Y Y, YANG J P, WANG Q H, et al.The effects of high temperature stress on ultraweak luminescence and some physiological characters in Chinese cabbage[J]. Acta Agriculturae Boreali-Occidentalis Sinica, 2006, 15(5): 218-221.(in Chinese with English abstract)
[8] 闫圆圆, 曾爱松, 宋立晓, 等. 结球甘蓝幼苗耐热性鉴定方法及耐热生理[J]. 江苏农业学报, 2016, 32(4): 885-890.
YAN Y Y, ZENG A S, SONG L X, et al.Identification of heat tolerance in cabbage seedlings and heat-tolerant physiology[J]. Jiangsu Journal of Agricultural Sciences, 2016, 32(4): 885-890.(in Chinese with English abstract)
[9] 汪炳良, 徐敏, 史庆华, 等. 高温胁迫对早熟花椰菜叶片抗氧化系统和叶绿素及其荧光参数的影响[J]. 中国农业科学, 2004, 37(8): 1245-1250.
WANG B L, XU M, SHI Q H, et al.Effects of high temperature stress on antioxidant systems, chlorophyll and chlorophyll fluorescence parameters in early cauliflower leaves[J]. Scientia Agricultura Sinica, 2004, 37(8): 1245-1250.(in Chinese with English abstract)
[10] 高桂珍. 油菜种子耐热性的鉴定及分子机理研究[D]. 北京: 中国农业科学院, 2015.
GAO G Z.Studies on identification and molecular mechanism of seed heat tolerance in rapeseed[D]. Beijing: Chinese Academy of Agricultural Sciences, 2015.(in Chinese with English abstract)
[11] 张景云, 赵晓东, 万新建, 等. 小白菜耐热性鉴定及其耐热性分析[J]. 核农学报, 2014, 28(1): 146-153.
ZHANG J Y, ZHAO X D, WAN X J, et al.Identification and analysis of heat tolerance identification and analysis for Brassica campestris ssp. chinensis[J]. Journal of Nuclear Agricultural Sciences, 2014, 28(1): 146-153.(in Chinese with English abstract)
[12] 李合生,孙群,赵世杰. 植物生理生化实验原理和技术[M]. 北京: 高等教育出版社, 2000.
[13] 屠小菊, 汪启明, 饶力群. 高温胁迫对植物生理生化的影响[J]. 湖南农业科学, 2013(13): 28-30.
TU X J, WANG Q M, RAO L Q.Effects of high temperature stress on physiology and biochemistry of plant[J]. Hunan Agricultural Sciences, 2013(13): 28-30.(in Chinese with English abstract)
[14] 张梦如, 杨玉梅, 成蕴秀, 等. 植物活性氧的产生及其作用和危害[J]. 西北植物学报, 2014, 34(9): 1916-1926.
ZHANG M R, YANG Y M, CHENG Y X, et al.Generation of reactive oxygen species and their functions and deleterious effects in plants[J]. Acta Botanica Boreali-Occidentalia Sinica, 2014, 34(9): 1916-1926.(in Chinese with English abstract)
[15] 夏钦, 何丙辉, 刘玉民, 等. 高温胁迫对粉带扦插苗形态和生理特征的影响[J]. 生态学报, 2010, 30(19):5217-5224.
XIA Q, HE B Y, LIU Y M, et al.Effects of high temperature stress on the morphological and physiological characteristics in Scaevola albida cutting seedlings[J]. Acta Ecologica Sinica, 2010, 30(19):5217-5224. (in Chinese with English abstract)
[16] 孙军利, 赵宝龙, 郁松林. 外源水杨酸对高温胁迫下葡萄幼苗膜脂过氧化及抗氧化酶活性的影响[J]. 石河子大学学报(自然科学版), 2015, 33(3): 275-280.
SUN J L, ZHAO B L, YU S L.Effects of exogenous salicylic acid(SA) on membrane lipid peroxidation and the activity of antioxidase in grape seedlings under high temperature stress[J]. Journal of Shihezi University(Natural Science), 2015, 33(3): 275-280.(in Chinese with English abstract)
[17] 宋云鹏, 刘凯歌, 龚繁荣. 不同生菜品种苗期耐热性的综合评价[J]. 浙江农业学报, 2015, 27(2): 176-181.
SONG Y P, LIU K G, GONG F R.Comprehensive evaluation on heat tolerance of various lettuce seedlings[J]. Acta Agriculturae Zhejiangensis, 2015, 27(2): 176-181.(in Chinese with English abstract)
[18] 王娟, 寿森炎. 高温胁迫对莴笋幼苗叶绿素和抗氧化系统的影响[J]. 北方园艺, 2007(2): 1-5.
WANG J, SHOU S Y.Effects of high temperature stress on chlorophyll and antioxidant systems in lettuce seedlings[J]. Northern Horticulture, 2007(2): 1-5.(in Chinese with English abstract)
[19] 吴雪霞, 查丁石, 朱宗文, 等. 外源24-表油菜素内酯对高温胁迫下茄子幼苗生长和抗氧化系统的影响[J]. 植物生理学报, 2013, 49(9): 929-934.
WU X X, ZHA D S, ZHU Z W, et al.Effects of exogenous 24-epibrassinolide on plant growth and antioxidant system in eggplant seedlings under high temperature stress[J]. Plant Physiology Journal, 2013, 49(9): 929-934.(in Chinese with English abstract)
[20] 刘凯歌, 宋云鹏, 龚繁荣, 等. 高温胁迫对甜椒幼苗生长和生理生化指标的影响[J]. 上海农业学报, 2015, 31(3): 63-67.
LIU K G, SONG Y P, GONG F R, et al.Effects of high temperature stress on the growth and physiological and biochemical indexes of sweet pepper seedlings[J]. Acta Agriculturae Shanghai, 2015, 31(3): 63-67.(in Chinese with English abstract)
[21] 王浩伟, 陈国菊, 陈长明, 等. 芥蓝耐热性鉴定及耐热转录因子MBF1c表达分析[J]. 中国蔬菜, 2017(2): 30-37.
WANG H W, CHEN G J, CHEN C M, et al.Identification of heat tolerance in Chinese kale and the expression analysis of heat tolerance transcription factor MBF1c[J]. China Vegetables, 2017(2): 30-37.(in Chinese with English abstract)
[22] ZHANG H J, ZHANG N, YANG R C, et al.Melatonin promotes seed germination under high salinity by regulating antioxidant systems, ABA and GA₄ interaction in cucumber (Cucumis sativus L.)[J]. Journal of Pineal Research, 2014, 57(3): 269-279.
[23] 高俊杰, 秦爱国, 于贤昌. 低温胁迫下嫁接对黄瓜叶片SOD和CAT基因表达与活性变化的影响[J]. 应用生态学报, 2009, 20(1): 213-217.
GAO J J, QIN A G, YU X C.Effects of grafting on cucumber leaf SOD and CAT gene expression and activities under low temperature stress[J]. Chinese Journal of Applied Ecology, 2009, 20(1): 213-217.(in Chinese with English abstract)
[24] NEGI N P, SHRIVASTAVA D C, SHARMA V, et al.Overexpression of CuZnSOD from Arachis hypogaea alleviates salinity and drought stress in tobacco[J]. Plant Cell Reports, 2015, 34(7): 1109-1126.

菜心耐热性评价及酶促抗氧化系统对高温胁迫的响应

Evaluation of heat tolerance and response of enzymatic antioxidant system to heat stress in Brassica parachinensis L.

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