紫茎泽兰透皮剂的制备工艺优化及其临床杀螨效果观察

doi:10.3969/j.issn.1004-1524.2018.04.08

浙江农业学报 ›› 2018, Vol. 30 ›› Issue (4): 576-585.DOI: 10.3969/j.issn.1004-1524.2018.04.08

紫茎泽兰透皮剂的制备工艺优化及其临床杀螨效果观察

王柳杰¹, 房春林², 古小彬¹, 谢跃¹, 杨光友^{1, *}

1.四川农业大学动物医学院,四川成都611130;
2.成都农业科技职业学院畜牧兽医分院,四川成都 611130

收稿日期:2017-09-11 出版日期:2018-04-20 发布日期:2018-04-19
通讯作者: 杨光友,E-mail:guangyou1963@aliyun.com
作者简介:王柳杰(1989—),女,四川成都人,硕士,从事动物寄生虫病学研究。E-mail:1096024143@qq.com
基金资助:
四川农业大学双支计划(SCU03572432)

Optimizing transdermal agent preparation process of Eupatorium adenophorum and evaluating its effectiveness against Psoroptes cuniculi and Sarcoptes scabiei in vivo

WANG Liujie¹, FANG Chunlin², GU Xiaobin¹, XIE Yue¹, YANG Guangyou^{1, *}

1. College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China;
2. Animal Husbandry and Veterinary Branch, Chengdu Agricultural Science and Technology Vocational College, Chengdu 611130, China

Received:2017-09-11 Online:2018-04-20 Published:2018-04-19

摘要/Abstract

摘要： 以紫茎泽兰杀螨活性主成分紫茎泽兰素A(9-羰基-10,11-去氢泽兰酮,euptox A)提取率为考查指标,对影响提取率的乙醇体积分数、料液比、提取时间、提取次数等因素在单因素试验的基础上进行正交试验优选,确定紫茎泽兰乙醇最佳提取工艺为:加20倍量(体积质量比)的95%乙醇,回流提取3次,每次1 h。将以最佳提取工艺条件提取的紫茎泽兰乙醇提取液制成加或不加氮酮的杀螨透皮剂,进行家兔皮肤的急性毒性试验和刺激性试验。结果表明,家兔完整皮肤和破损皮肤在给药72 h后没有出现急性毒性反应和刺激性。另开展临床杀螨治疗效果试验,将30只痒螨兔和26只疥螨兔分别分成6组,A、B、C、D组分别以加氮酮紫茎泽兰生药含量1.0 g·mL^-1、0.5 g·mL^-1和不加氮酮生药含量为1.0 g·mL^-1、0.5 g·mL^-1的杀螨液涂擦处理,E组为阳性对照组,注射伊维菌素0.2 mg,F组为空白对照组,涂擦0.9%生理盐水。试验结果表明,不论痒螨组还是疥螨组,杀螨效果最好的都是加氮酮高剂量组(A组)。

关键词: 紫茎泽兰, 提取工艺, 临床杀螨, 杀螨液, 皮肤安全性试验

Abstract: To screen the extraction process of Eupatorium adenophorum and evaluate its acaricidal activity in vivo, 9-oxo-10,11-dehydro ageraphorone (euptox A), which is the main acaricidal active ingredient, was chosen as the detective index, and single factor level test and orthogonal test were carried out to investigate the influence of volume percentage of ethanol, solid to liquid ratio, extraction duration and extraction times on the extraction percentage. The optimal extraction conditions for ethanol extraction were proposed as: 95% volume percentage of ethanol, solid to liquid ratio of 1:20, extraction duration of 1 h, extraction times of three. The skin acute toxicity test and skin irritation test were carried out by sapreading the acaricidal preparations of E. adenophorum on the integral and damaged back skin of healthy rabbits. The results showed that after using the drug for 72 h, rabbits did not show any reaction of acute toxicity and irritation. In acaricidal clinical test, 30 rabbits with psoroptes mites and 26 rabbits with scabies mites were respectively divided into six groups. The animals in group A and B were treated with the acaricidal preparation contained azone and raw drug (E. adenophorum) 1.0, 0.5 g·mL^-1, respectively. The animals in groups C and D were treated with the acaricidal preparation contained raw drug 1.0, 0.5 g·mL^-1, which did not contain azone, respectively. The animals in group E and F were injected with ivermectin 0.2 mg (positive controls) and embrocated 0.9% normal saline on skin (negative controls), respectively. The results showed that,whether for the psoroptes mite group or the scabies mite group, the prepatation with high dose of E. adenophorum and azone worked best.

Key words: Eupatorium adenophorum, extraction process, clincal acariasis, acaricidal preparation, skin safety test

中图分类号:

王柳杰, 房春林, 古小彬, 谢跃, 杨光友. 紫茎泽兰透皮剂的制备工艺优化及其临床杀螨效果观察[J]. 浙江农业学报, 2018, 30(4): 576-585.

WANG Liujie, FANG Chunlin, GU Xiaobin, XIE Yue, YANG Guangyou. Optimizing transdermal agent preparation process of Eupatorium adenophorum and evaluating its effectiveness against Psoroptes cuniculi and Sarcoptes scabiei in vivo[J]. , 2018, 30(4): 576-585.

参考文献

[1] 张吉丽,李冰,张继瑜. 动物抗寄生虫药物的研究与应用进展[J]. 黑龙江畜牧兽医,2016,17(9):74-79.
ZHANG J L, LI B, ZHANG J Y. Progress in research and application of animal antiparasitic drugs[J]. Heilongjiang Animal science and Veterinary Medicine , 2016, 17(9):74-79.(in Chinese with English abstract)
[2] 王建芬,丁双阳,王晓琳. 含氟杀虫剂类农药的残留检测分析研究进展[J]. 中国农学通报,2016,32(2):68-72.
WANG J F, DING S Y, WANG X L. Research progress of analytical and detection methods of fluorine-containing pesticides residues[J]. Chinese Agricultural Science Bulletin , 2016, 32(2):68-72.(in Chinese with English abstract)
[3] 邵仁志,刘小安,孙兰,等. 中国植物源农药的研究进展[J]. 湖北农业科学,2017,56(8):1401-1405.
SHAO R Z, LIU X A, SUN L, et al. Progresses in botanical pesticides in China[J]. Hubei Agricultural Sciences , 2017, 56(8):1401-1405.(in Chinese with English abstract)
[4] 程茂高,乔卿梅,魏志华,等. 中药材仓储害虫及其绿色防控技术研究进展[J]. 中药材,2016,39(8):1917-1921.
CHENG M G, QIAO Q M, WEI Z H, et al. Research progress of Chinese herbal medicine storage pests and green control technology[J]. Journal of Chinese Medicinal Materials , 2016, 39(8):1917-1921.(in Chinese)
[5] 赵鸿峥,骆骄阳,刘秋桃,等. 药用植物挥发油驱蚊作用的研究进展[J]. 中国中药杂志,2016,41(1):28-34.
ZHAO H Z, LUO J Y, LIU Q T, et al. Study on essential oils of medicinal plants in insect repellen[J]. China Journal of Chinese Materia Medica , 2016, 41(1):28-34.(in Chinese with English abstract)
[6] REN Q, CAO L Z, SU J W, et al. Volatile emissions from the invasive weed Eupatorium adenophorum induced by Aphis gossypii feeding and methyl jasmonate treatment[J]. Weed Science , 2010, 58(3):252-257.
[7] SHARMA S, MEHTA P K. Evaluation of plant extracts on the oviposition deterrent and adult emergence activities of diamondback moth ( Pluiella xylostella )[J]. Pestology . 2006, 30(8):11-13.
[8] 刘燕萍, 高平, 潘为高,等. 紫茎泽兰等几种植物提取物对两种农业害螨的毒力作用研究[J]. 四川大学学报(自然科学版),2004, 41(1):212-215.
LIU Y P, GAO P, PAN W G, et al. Effect of several plant extracts on Tetranychus ucticae and Panoychus [J]. Journal of Sichuan University ( Natural Science Edition ), 2004, 41 (1):212-215.(in Chinese with English abstract)
[9] 李云寿,邹华英,佴注,等. 紫茎泽兰精油各馏分对4种仓库害虫的杀虫活性[J]. 西南农业大学学报(自然科学版),2000,22(4):331-332.
LI Y S, ZOU H Y, NAI Z, et al. Isecticidal acivity of different fractions of essential oils extracted from Eupatorium adeophorum against 4 species of stored grain insects[J]. Journal of Southwest University ( Natural Science Edition ), 2000, 22(4):331-332.(in Chinese with English abstract)
[10] YANG J F, WANG R Q, LV R, et al. Anticoccidial activity of Eupatorium adenophorum extracts against chicken coccidian oocysts[J]. Journal of Animal and Veterinary Advances , 2012, 11(8):1255-1257.
[11] 赵平,王荣琼,杨晓乾,等. 恒温条件下紫茎泽兰花、叶提取液对马圆线虫虫卵的孵化试验[J]. 山东畜牧兽医,2010,31(11):2-3.
ZHAO P, WANG R Q, YANG X Q, et al. The effect of Eupatorium adenophorum flower and leaf extract on hatching the eggs of Caenorhabditis elegans under the condition of constant temperature[J]. Shandong Journal of Animal Science and Veterinary Medicine , 2010, 31(11):2-3.(in Chinese)
[12] SHABA P, PANDEY N N, SHARMA O P, et al. Therapeutic activity of methanolic extract of Eupatorium adenophorum leaves against Trypanosoma Evansi [J]. International Journal of Basic and Applied Medical Sciences , 2012, 2(1):77-82.
[13] NONG X, FANG C L, WANG J H, et al. Acaricidal activity of extract from Eupatorium adenophorum against the Psoroptes cuniculi and Sarcoptes scabiei in vitro [J]. Veterinary Parasitology , 2012, 187(1/2):345-349.
[14] NONG X, REN Y J, WANG J H, et al. Clinical efficacy of botanical extracts from Eupatorium adenophorum against the Sarcoptes scabiei (Sarcoptidae:Sarcoptes) in rabbits[J]. Veterinary Parasitology , 2013, 195(1/2):157-164.
[15] NONG X, REN Y J, WANG J H, et al. Clinical efficacy of botanical extracts from Eupatorium adenophorum against the scab mite, Psoroptes cuniculi [J]. Veterinary Parasitology , 2013, 192(1/3):247-252.
[16] NONG X, LI S H, WANG J H, et al. Acaricidal activity of petroleum ether extracts from Eupatorium adenophorum against the ectoparasitic cattle mite, Chorioptes texanus [J]. Parasitology Research , 2014, 113(3):1201-1207.
[17] NONG X, TAN Y J, WANG J H, et al. Evaluation acaricidal efficacy of botanical extract from Eupatorium adenophorum against the hard tick Haemaphysalis longicornis (Acari:Ixodidae)[J]. Experimental Parasitology , 2013, 135(3):558-563.
[18] NONG X, CHEN F Z, YANG Y J, et al. Aphicidal activity of an ageraphorone extract from Eupatorium adenophorum against Pseudoregma bambucicola (Homoptera: Aphididae, Takahashi)[J]. Journal of Insect Science , 2015, 15(1):81.
[19] 郝光荣. 实验动物学[M]. 2版. 上海:第二军医大学出版社,2001:184-185.
[20] 中华人民共和国卫生部药政局. 中药新药研究指南(药学、药理学、毒理学)[M]. 北京:人民出版社,1994:209-212.
[21] 国家食品药品监督局. 中药、天然药药物研究技术指导原则[M]. 北京:中国医药科技出版社,2005:108.
[22] 于泓. 皮肤医学美容学[M]. 北京:中国医学科技出版社,1997:78-81.
[23] PERRUCCI S, FLAMINI G, CIONI P L, et al. In vitro and in vivo efficacy of extracts of Artemisia verlotorum against Psoroptes cuniculi [J]. Veterinary Record , 2001, 148(26):814-815.
[24] WALTON S F, CURRIE B J. Problems in diagnosing scabies, a global disease in human and animal populations[J]. Clinical Microbiology Reviews , 2007, 20(2):268-279.
[25] TABASSAM S M, IQBAL Z, JABBAR A, et al. Efficacy of crude neem seed kernel extracts against natural infestation of Sarcoptes scabiei var. ovis [J]. Journal of Ethnopharmacology , 2008, 115(2):284-287.
[26] GLANTZ S A. Statistica per discipline biomediche[M]. 5th rev. Milan, Italy: McGraw-Hill, 2003.
[27] RIM I S, JEE C H. Acaricidal effects of herb essential oils against Dermatophagoides farinae and D. pteronyssinus (Acari: Pyroglyphidae) and qualitative analysis of a herb Mentha pulegium (pennyroyal)[J]. Korean Journal of Parasitology , 2006, 44(2):133-138.
[28] SAS INSTITUTE INC. Base SAS 9.4 Procedures Guide: Statistical Procedures[M]. Cary, NC, USA: SAS Institute Inc, 2014.
[29] OELRICHS P B, CALANASAN C A, MACLEOD J K, et al. Isolation of a compound from Eupatorium adenophorum , (Spreng.) [ Ageratina adenophora , (Spreng)] causing hepatotoxicity in mice[J]. Natural Toxins , 1995, 3(5):350-354.
[30] 白洁,曹坳程,郭美霞,等. 紫茎泽兰9-羰基-10,11-去氢泽兰酮分布积累动态[J]. 植物学报,2011,46(4):470-475.
BAI J, CAO A C, GUO M X, et al. Identification of 9-oxo-10,11-dehydroagerophorone in Eupatorium adenophorum by high performance liquid chromatography[J]. Chinese Bulletin of Botany , 2011, 46(4):470-475.(in Chinese)
[31] NONG X, LI S H, CHEN F Z, et al. Isolation and identification of acaricidal compounds in Eupatorium adenophorum ,petroleum ether extract and determination of their acaricidal activity against Psoroptes cuniculi [J]. Veterinary Parasitology , 2014, 203(1/2):197-202.
[32] HU Y, LIAO F, HU Y, et al. Clinical efficacy of 9-oxo-10,11-dehydroageraphorone extracted from Eupatorium adenophorum against Psoroptes cuniculi in rabbits[J]. BMC Veterinary Research , 2014, 10:970.
[33] LIAO F, HU Y, TAN H, et al. Acaricidal activity of 9-oxo-10, 11-dehydroageraphorone extracted from Eupatorium adenophorum in vitro[J]. Experimental Parasitology , 2014, 140(1):8-11.
[34] 姜少娟,陈豪,李飞. 紫茎泽兰不同溶剂提取物的抑菌活性[J]. 江苏农业科学,2013,41(7):124-126.
JIANG S J, CHEN H, LI F. Antibacterial activity of different solvent extracts from Eupatorium adenophorum [J]. Jiangsu Agricultural Sciences , 2013, 41(7):124-126.(in Chinese)
[35] LEE P J, LANGER R, SHASTRI V P. Novel microemulsion enhancer formulation for simultaneous transdermal delivery of hydrophilic and hydrophobic drugs[J]. Pharmaceutical Research , 2003, 20(2):264-269.
[36] 李国锋,陈业豪,刘立捷,等. 角质层与活性皮肤层对硝酸异山梨醋透皮吸收的影响[J]. 中国药师,2002,5(4):199-201.
LI G F, CHEN Y H, LIU L J, et al. Influence of stratum corneum and viable layer of skin on the iransdermal absorption of isosorbide dinitrate[J]. China Pharmacist , 2002, 5(4):199-201.(in Chinese with English abstract)
[37] 曹允,王黎霞,雷莉辉,等. 透皮贴剂在兽药制剂领域的研究进展[J]. 黑龙江畜牧兽医,2016(21):70-72.
CAO Y, WANG L X, LEI L H, et al. Research progress of transdermal patches in veterinary drug preparations[J]. Heilongjiang Animal Science and Veterinary Medicine , 2016(21):70-72.(in Chinese)
[38] 王锐,郭俊华,张远丹. 皮酚体外透皮吸收研究[J]. 中草药,2000,31(1):42-44.
WANG R, GUO J H, ZHANG Y D. In vitro study on the transdermal absorption of paeonol[J]. Chinese Traditional and Herbal Drugs , 2000, 31(1):42-44.(in Chinese)
[39] TSAI Y H, LEE K F, HUANG Y B, et al. In vitro permeation and in vivo whitening effect of topical hesperetin microemulsion delivery system[J]. International Journal of Pharmaceutics , 2010, 388(1/2):257-262.
[40] MOGHIMIPOUR E, SALIMI A, KARAMI M, et al. Preparation and characterization of dexamethasone microemulsion based on pseudoternary phase diagram[J]. Jundishapur Journal of Natural Pharmaceutical Products , 2013, 8(3):105-112.

紫茎泽兰透皮剂的制备工艺优化及其临床杀螨效果观察

Optimizing transdermal agent preparation process of Eupatorium adenophorum and evaluating its effectiveness against Psoroptes cuniculi and Sarcoptes scabiei in vivo

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 1

编辑推荐

Metrics

本文评价