古茶树优系矢车菊素分析

doi:10.3969/j.issn.1004-1524.2018.04.09

浙江农业学报 ›› 2018, Vol. 30 ›› Issue (4): 586-591.DOI: 10.3969/j.issn.1004-1524.2018.04.09

古茶树优系矢车菊素分析

成凯^{1, 2}, 刘进平^{1, 2}, 邓代信^{1, 2}, 陈正武³, 李忠朝⁴, 周绍均^{5, 6}, 牛素贞^{7, *}

1.贵州大学农学院, 贵州贵阳 550025;
2.贵州大学园艺专业学科实验室, 贵州贵阳 550025;
3.贵州省茶叶研究所, 贵州贵阳 550025;
4.都匀市科技和知识产权局, 贵州都匀 558000;
5.遵义市产品质量检验检测院, 贵州遵义 563000;
6.国家茶及茶制品质量监督检验中心(贵州), 贵州遵义 563000;
7.贵州大学茶学院, 贵州贵阳 550025

收稿日期:2017-06-26 出版日期:2018-04-20 发布日期:2018-04-19
通讯作者: 牛素贞,E-mail:niusuzhen@163.com
作者简介:成凯(1991—),男,贵州赫章人,硕士,主要从事园艺植物栽培及基础生理生化研究工作。E-mail:cheng0406kai@163.com
基金资助:
国家自然科学基金(31560222); 贵州省科研机构服务企业行动计划项目黔科合服企(〔2014〕4008号); 贵州省教育厅自然科学研究项目(〔2014〕2995号); 贵州大学引进人才科研项目(贵大人基合字〔2014〕19号); 贵州省科技计划项目(黔科合支撑〔2017〕2558)

Analysis on cyanidin content of ancient tea tree superior lines

CHENG Kai^{1, 2}, LIU Jinping^{1, 2}, DENG Daixin^{1, 2}, CHEN Zhengwu³, LI Zhongchao⁴, ZHOU Shaojun^{5, 6}, NIU Suzhen^{7, *}

1. College of Agriculture, Guizhou University, Guiyang 550025, China;
2. Horticultural Major Laboratory, Guizhou University, Guiyang 550025, China;
3. Guizhou Tea Research Institute, Guiyang 550025, China;
4.Duyun City Science and Intellectual Property Bureau, Duyun 558000, China;
5.Product Quality Inspection Laboratory of Zunyi, Zunyi 563000, China;
6. National Tea Quality Supervision and Inspection Center(Guizhou), Zunyi 563000, China;
7.College of Tea, Guizhou University, Guiyang 550025, China

Received:2017-06-26 Online:2018-04-20 Published:2018-04-19

摘要/Abstract

摘要： 采用高效液相色谱法,以春梢一芽二叶为研究对象,定量分析4个古茶树优系矢车菊素的含量。结果表明,高效液相色谱仪测定古茶树矢车菊素含量最合适的流动相A为超纯水,流动相B为乙腈,体积比为1:9。古茶4号的矢车菊素含量最高,而古茶2号矢车菊素含量较低,且均显著高于或低于对照福鼎大白茶。

关键词: 高效液相色谱法, 古茶树, 矢车菊素

Abstract: The content of cyanidin of the ancient tea tree superior lines was analyzed by high performance liquid chromatography (HPLC). The results showed that the optimal mobile phase of HPLC analysis on the content of cyanidin of the ancient tea superior lines consisted of acetonitrile and ultrapure water (9:1, V/V). Compared with the control Fuding Dabaicha, line 4 of ancient tea tree had the highest content of cyanidin and line 2 had the lowest content of cyanidin, which both showed the significant difference from the control.

Key words: high performance liquid chromatography (HPLC), ancient tea tree, cyanidin

中图分类号:

S571.1

成凯, 刘进平, 邓代信, 陈正武, 李忠朝, 周绍均, 牛素贞. 古茶树优系矢车菊素分析[J]. 浙江农业学报, 2018, 30(4): 586-591.

CHENG Kai, LIU Jinping, DENG Daixin, CHEN Zhengwu, LI Zhongchao, ZHOU Shaojun, NIU Suzhen. Analysis on cyanidin content of ancient tea tree superior lines[J]. , 2018, 30(4): 586-591.

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古茶树优系矢车菊素分析

Analysis on cyanidin content of ancient tea tree superior lines

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