致仔猪腹泻大肠埃希菌的分离及耐药性分析

doi:10.3969/j.issn.1004-1524.2018.04.07

浙江农业学报 ›› 2018, Vol. 30 ›› Issue (4): 568-575.DOI: 10.3969/j.issn.1004-1524.2018.04.07

致仔猪腹泻大肠埃希菌的分离及耐药性分析

王婧¹, 刁小龙², 杨茂义¹, 王全方¹, 陈晓兰¹, 张龙^{1, *}

1.江苏农牧科技职业学院,江苏泰州 225300;
2.中崇信诺生物科技泰州有限公司,江苏泰州 225300

收稿日期:2017-07-31 出版日期:2018-04-20 发布日期:2018-04-19
通讯作者: 张龙,E-mail:longz98@163.com
作者简介:王婧(1985—),女,四川绵阳人,博士,讲师,主要从事兽药药理研究。E-mail:kenhtsjj@163.com
基金资助:
江苏农牧科技职业学院院级项目(NSF201504-2); 江苏省2016大学生创新创业训练计划项目(201612806020X); 泰州市农业支持项目(TN201519)

Isolation and susceptibility test of pathogens responsible for porcine postweaning diarrhea

WANG Jing¹, DIAO Xiaolong², YANG Maoyi¹, WANG Quanfang¹, CHEN Xiaolan¹, ZHANG Long^{1, *}

1. Jiangsu Agri-Animal Husbandry Vocational College, Taizhou 225300, China;
2. Zhongchong Sino Biotech Taizhou Co., Ltd, Taizhou 225300, China

Received:2017-07-31 Online:2018-04-20 Published:2018-04-19

摘要/Abstract

摘要： 为调查江苏省泰州地区引起仔猪腹泻的主要病原,采集腹泻仔猪的肛拭子样品共70份,利用MA琼脂平板共分离得到136株细菌。鉴定结果表明,其中含大肠埃希菌119株、沙门氏菌5株、志贺菌4株、阪崎肠杆菌8株。致病性试验确定其中有81株细菌为致病性大肠埃希菌,对其进行O抗原血清型鉴定,有36株分离菌被定型,分属于6种血清型,以O₇₈和O₁₀₁为优势血清型。进一步对分离得到的大肠埃希菌进行肠毒素基因检测,结果表明,这些大肠埃希菌中包含ST1+ST2型20株、LT1+ST2型44株、ST2型17株,共3种产肠毒素类型。药敏试验结果显示,超过75%的菌株对头孢他啶、阿米卡星和氧氟沙星表现为敏感,而对常用的四环素、头孢呋辛、卡那霉素、多西环素等呈现耐药性,且存在严重的多药耐药现象。研究结果可为泰州地区仔猪腹泻的预防和治疗提供参考依据。

关键词: O抗原, 药敏试验, 耐药性, 肠毒素基因

Abstract: In order to investigate the main causes of diarrhea of piglets in Taizhou, Jiangsu Province, 70 rectal swab samples were collected from the live diarrheic piglets, and 136 strains of bacteria were isolated by MA plates, in which 119 strains of Escherichia coli, 5 strains of Salmonella, 4 strains of Shigella, 8 strains of Cronobacter sakazakii were characterized by biochemical identification and 16S rDNA gene sequence analysis. 81 strains of pathogenic E. coli were confirmed according to the animal test. Among them, 36 strains of pathogenic E. coli were identified belonging to 6 serotypes by agglutination tests, and the predominant serotypes were O₇₈ and O₁₀₁. In addition, among 81 pathogenic E. coli, 20 isolates were ST1+ST2, 44 isolates were LT1+ST2,while others were ST2. The drug resistance of pathogenic isolates were tested by Kirby-Barer method, and the results showed that more than 75% pathogenicity isolates were sensitive to ceftazidime, amikacin and ofloxacin, but resistant to tetracycline, cefuroxime, kanamycin and doxycycline seriously,and there were serious multi-drug resistance. These results would provide scientific data for the prevention and control of porcine postweaning diarrhea disease in Taizhou area.

Key words: O-antigen, susceptibility test, drug resistance, enterotoxin gene

中图分类号:

S856

王婧, 刁小龙, 杨茂义, 王全方, 陈晓兰, 张龙. 致仔猪腹泻大肠埃希菌的分离及耐药性分析[J]. 浙江农业学报, 2018, 30(4): 568-575.

WANG Jing, DIAO Xiaolong, YANG Maoyi, WANG Quanfang, CHEN Xiaolan, ZHANG Long. Isolation and susceptibility test of pathogens responsible for porcine postweaning diarrhea[J]. , 2018, 30(4): 568-575.

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致仔猪腹泻大肠埃希菌的分离及耐药性分析

Isolation and susceptibility test of pathogens responsible for porcine postweaning diarrhea

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