浙江农业学报 ›› 2018, Vol. 30 ›› Issue (4): 560-567.DOI: 10.3969/j.issn.1004-1524.2018.04.06

• 动物科学 • 上一篇    下一篇

大豆抗原蛋白对仔猪的免疫作用

张瑜, 王元红, 汤雪冰, 彭成璐, 舒迎霜, 曹程鸣, 冯士彬, 李玉, 王希春, 吴金节*   

  1. 安徽农业大学 动物科技学院,安徽 合肥 230036
  • 收稿日期:2017-08-14 出版日期:2018-04-20 发布日期:2018-04-19
  • 通讯作者: 吴金节,E-mail:wjj@ahau.edu.cn
  • 作者简介:张瑜(1993—),女,江苏张家港人,硕士研究生,从事兽医临床诊疗技术研究。E-mail:1121514285@qq.com
  • 基金资助:
    科技部科技富民强县专项行动计划(国科发农〔2012〕745号); 国家级大学生创新创业训练计划(教高司函〔2016〕45号)

Immune effect of soybean antigen protein in piglets

ZHANG Yu, WANG Yuanhong, TANG Xuebing, PENG Chenglu, SHU Yingshuang, CAO Chengming, FENG Shibin, LI Yu, WANG Xichun, WU Jinjie*   

  1. College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China
  • Received:2017-08-14 Online:2018-04-20 Published:2018-04-19

摘要: 通过口服或注射两种免疫途径研究大豆抗原蛋白免疫剂对仔猪血清中大豆抗原蛋白抗体效价及IgA、IgM、IgE、IgG和IL-4水平的影响。选取70头7日龄“杜×长×大”杂交仔猪,随机分成A组(对照)、B组[β-伴大豆球蛋白(7S)致敏]、C组[大豆球蛋白(11S)致敏]、D组(7S口服免疫+致敏)、E组(11S口服免疫+致敏)、F组(7S注射免疫+致敏)和G组(11S注射免疫+致敏),每组10头。D、F组和E、G组仔猪在7日龄时按分组设计,口服或注射纯化的11S或7S免疫剂,A组注射生理盐水;在14日龄时再免疫一次。所有仔猪于21日龄断奶。从21日龄开始,A组仔猪饲喂基础日粮,B、D、F组和C、E、G组仔猪饲喂分别添加5% 7S或5% 11S的基础日粮,连续饲喂7 d。在27日龄时,对仔猪进行皮肤致敏试验。在 7、14、21、27日龄时,所有仔猪前腔静脉采血,采用ELISA法检测血清中IgA、IgM、IgE、IgG和IL-4水平,采用琼脂扩散法测定血清中7S和11S的抗体效价。结果显示,仔猪皮肤致敏评判结果B、C组>D、E组>A、F、G组,且7S致敏性高于11S。在21日龄时,F、G组血清中抗体效价高于D、E组;在27日龄时,B、C组血清中抗体效价高于D、E、F、G组。在14日龄时,F组与A组相比,IgG、IL-4水平显著(P<0.05)升高;在21日龄时,D、E、F、G组的IgM、IgE、IgG、IL-4水平与A、B、C组相比显著(P<0.05)升高,除了G组IgG外,F、G组又显著(P<0.05)高于D、E组;在27日龄时,各处理组IgE、IgG和IL-4水平极显著(P<0.01)高于A组,且B组显著(P<0.05)高于C组,D、E组显著(P<0.05)高于F、G组。试验结果证实,7S对仔猪的致敏作用高于11S,大豆抗原蛋白免疫剂对仔猪具有一定的免疫保护作用,且对7S的免疫效果优于11S,注射免疫的效果要优于相同剂量的口服免疫。

关键词: 大豆球蛋白(11S), β, -伴大豆球蛋白(7S), IgA, IgM, IgE, IgG, IL-4

Abstract: Piglets were immunized by oral administrating or injecting soybean antigen protein immunizing agents to study the effects of soybean antigen protein immunizing agents on serum antibody titers of β-conglycinin (7S), glycinin (11S), and serum IgA, IgM, IgE, IgG and IL-4 levels in piglets. Seventy “Duroc×Landrace×Yorkshire” crossbred piglets (7-day-old) were randomly allocated into group A (control group), group B (7S sensitized group), group C (11S sensitized group), group D (7S orally immunized +sensitized group), group E (11S orally immunized +sensitized group), group F (7S injecting immunized +sensitized group) and group G (11S injecting immunized + sensitized group) with ten piglets each. Piglets were orally taken or injected with soybean antigen protein immunizing agents according to design at 7 days old and were immunized again at 14 days old, and the piglets in group A were injected with physiological saline. All the piglets were weaned at 21 days old. From 21 days old, piglets in group A were fed with basal diets, and piglets in group B, D, F and group C, E, G were fed with basal diets supplemented with 5% 7S or 5% 11S, respectively for 7 d. The skin sensitization test was conducted in piglets at 27 days old. Blood samples of ten piglets from each group were taken from vena cava anterior at 7, 14, 21 and 27 days old, and the sera was separated to measure IgA, IgM, IgE, IgG and IL-4 levels by ELISA method and antibody titers of 7S and 11S by agar diffusion method. The results showed that the skin sensitization valuing results of piglets were group B and C>group D and E>group A, F and G, meanwhile the sensitization effect of 7S was higher than that of 11S in piglets. Serum antibody titers in group F and G were higher than those in group D and E at the age of 21 days, and serum antibody titers in group B and C were higher than those in group D, E, F and G at the age of 27 days. Serum IgG, IL-4 levels of piglets in group F were significantly (P<0.05) higher than those in group A at 14 days old. Compared with group A, B and C, serum IgM, IgE, IgG and IL-4 levels of piglets were significantly (P<0.05) increased in group D, E, F and G at 21 days old, meanwhile those in group F and G were significantly (P<0.05) higher than those in group D and E, except IgG level in group G. At 27 days old, serum IgE, IgG and IL-4 levels of piglets in treatment groups were significantly (P<0.01) higher than those in group A, and those in group B were significantly (P<0.05) higher than those in group C, meanwhile those in group D and E were significantly (P<0.05) higher than those in group F and G. The experimental results suggested that the sensitization effect of 7S was higher than that of 11S in piglets. Soybean antigen protein immunizing agents could produce immune-protective effect in piglets, and the immune effect of 7S was prior to that of 11S. In addition, the effect of injecting immunization was prior to oral immunization with the same dose.

Key words: glycinin (11S), β-conglycinin(7S);, IgA, IgM, IgE, IgG, IL-4

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