回乳期奶牛血清GH、INS、HC、TGF-β1和IGF-1含量变化

doi:10.3969/j.issn.1004-1524.2017.04.05

浙江农业学报 ›› 2017, Vol. 29 ›› Issue (4): 548-554.DOI: 10.3969/j.issn.1004-1524.2017.04.05

回乳期奶牛血清GH、INS、HC、TGF-β1和IGF-1含量变化

沈留红¹, 巫晓峰¹, 肖劲邦¹, 姜思汛¹, 邓俊良¹, 左之才¹, 傅宏庆², 曹随忠¹, 余树民^{1, *}, 张有瑞³

1.四川农业大学动物医学院动物疫病与人类健康四川省重点实验室,奶牛疾病研究中心, 四川成都 611130;
2.江苏农牧科技职业学院,江苏泰州 225300;
3.河北省廊坊市农业局水产站,河北廊坊 065000

收稿日期:2016-11-01 出版日期:2017-04-20 发布日期:2017-04-27
通讯作者: 余树民,E-mail:yayushumin@163.com
作者简介:沈留红(1979—),男,江苏如皋人,副教授,博士研究生,从事反刍动物疾病及繁殖新技术研究,E-mail:shenlh@sicau.edu.cn
基金资助:
四川省教育厅重点项目(15ZA0024); 江苏农牧科技职业学院产业发展关键技术创新项目(NSF201604); 四川农业大学双支计划项目

Changes of GH, INS, HC, TGF-β1 and IGF-1 levels in cows’ serum during milk withdrawal period

SHEN Liuhong¹, WU Xiaofeng¹, XIAO Jinbang¹, JIANG Sixun¹, DENG Junliang¹, ZUO Zhicai¹, FU Hongqing², CAO Suizhong¹, YU Shumin^{1, *}, ZHANG Yourui³

1. The Key Laboratory of Animal Disease and Human Health of Sichuan Province, The Medical Research Center for Cow Disease,College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China;
2. Jiangsu Agri-animal Husbandry Vocational College, Taizhou 225300, China;
3. Agricultural Bureau of Langfang City, Langfang 065000, China

Received:2016-11-01 Online:2017-04-20 Published:2017-04-27

摘要/Abstract

摘要： 为探究回乳期奶牛血清中生长激素(GH)、胰岛素(INS)、氢化可的松(HC)、转化生长因子β1(TGF-β1)和胰岛素样生长因子1(IGF-1)含量的变化及其相关性。试验选择规模化奶牛场日产奶量为(15.43 ± 2.10) kg,即将干乳的妊娠后期健康中国荷斯坦奶牛20头。干乳开始当天记为第0天,分别采集第0、1、3、5、7、9和11天奶牛尾静脉血,ELISA法检测血清中GH、INS、HC、TGF-β1和IGF-1含量并对结果进行统计学处理,分析其在奶牛回乳过程中的变化规律及其相关性。结果显示,回乳期奶牛血清GH、INS、HC和TGF-β1含量在第0至1天变化不显著(P>0.05);血清GH和HC含量第3至11天依次降低且差异均极显著(P<0.01),其中血清HC含量第3天极显著高于其余各天(P<0.01);血清INS含量第3至11天依次升高且差异均极显著(P<0.01);血清TGF-β1含量第3至11天差异性均不显著(P>0.05);血清IGF-1含量依次呈阶梯式下降趋势,第0天和第1天、第3天和第5天、第7天和第9天、第9天和第11天之间均差异不显著(P>0.05),但第0至1天、第3至5天、第7至11天3阶段之间差异极显著(P<0.01)。回乳期奶牛血清GH、INS、TGF-β1和IGF-1含量变化两两间呈极显著相关(P<0.01);HC含量变化与GH、INS和IGF-1含量变化均呈极显著相关(P<0.01),与TGF-β1含量变化呈显著负相关(P<0.05)。

关键词: 回乳期奶牛, 生长激素, 胰岛素, 氢化可的松, 转化生长因子β, 1, 胰岛素样生长因子1

Abstract: In order to explore the changes and correlation among the levels of growth hormone (GH), insulin (INS), hydrocortisone (HC), TGF-β1 and IGF-1 in cows’ serum during the period of milk withdrawal. Twenty healthy Chinese Holstein cows in late pregnancy that gave (15.43±2.10) kg milk per day were used, which were ready to dry milk. The day when cows began to dry milk was recorded as the 0 d. The cows’ venous blood from the tail on the day 0, 1, 3, 5, 7, 9 and 11 were collected. ELISA was used to evaluate the levels of GH, INS, HC, TGF-β1 and IGF-1 in serum to analyze the changes and correlation among them. The results showed that, during the period of milk withdrawal, the changes of GH, INS, HC and TGF-β1 levels in serum were not obvious from the day 0 to the day 1 (P>0.05). The levels of GH and HC in serum were both on the decline and had significant difference from the day 3 to the day 11 (P<0.01), and the levels of GH in serum on the day 3 was significantly higher than those on the other days (P<0.01). The levels of INS in serum was on the rise and had significant (P<0.01) difference from the day 3 to the day 11, while the levels of TGF-β1 in serum had no significant (P>0.05) difference from the day 3 to the day 11. The levels of IGF-1 in serum showed downward trend in ladder type, and the difference among levels of IGF-1 in serum on the day 0 and day 1, day 3 and day 5, day 7 and day 9, day 9 and day 11 were not obvious (P>0.05), while IGF-1 levels among 0-1 d, 3-5 d and 7-11 d had significant (P<0.01) difference. There was significant (P<0.01) correlation among the levels of GH, INS, TGF-β1 and IGF-1 in cows’ serum separately during the period of milk withdrawal. The HC levels showed significant (P<0.01) correlation with GH, INS and IGF-1 levels, while it showed significant (P<0.05) negative correlation with TGF-β1 levels.

Key words: cows in milk withdrawal period, GH, INS, HC, TGF-β, 1, IGF-1

中图分类号:

S857.2

沈留红, 巫晓峰, 肖劲邦, 姜思汛, 邓俊良, 左之才, 傅宏庆, 曹随忠, 余树民, 张有瑞. 回乳期奶牛血清GH、INS、HC、TGF-β1和IGF-1含量变化[J]. 浙江农业学报, 2017, 29(4): 548-554.

SHEN Liuhong, WU Xiaofeng, XIAO Jinbang, JIANG Sixun, DENG Junliang, ZUO Zhicai, FU Hongqing, CAO Suizhong, YU Shumin, ZHANG Yourui. Changes of GH, INS, HC, TGF-β1 and IGF-1 levels in cows’ serum during milk withdrawal period[J]. , 2017, 29(4): 548-554.

参考文献

[1] 文静,卜登攀, 王建发, 等. 激素调控乳蛋白合成的作用及其分子机制[J]. 华北农学报, 2012, 27(增刊):111-115.
WEN J, BU D P, WANG J F, et al.Progress in the regulation role of lactoprotein synthesis by hormone and its molecular mechanism[J]. Acta Agriculturae Boreali-Sinica , 2012, 27(Suppl.): 111-115.(in Chinese with English abstract)
[2] OGATA Y, YU G M, HIDAKA T, et al. Effective embryo production from Holstein cows treated with gonadotropin-releasing hormone during early lactation [J]. Theriogenology , 2016, 86(6):1421-1426
[3] BACH L A. Insulin-like growth factor binding proteins—an update [J]. Pediatric Endocrinology Reviews : Per , 2015, 13(2):521-530.
[4] WANG J F, FU S P, LI S N, et al. Short-chain fatty acids inhibit growth hormone and prolactin gene transcription via cAMP/PKA/CREB signaling pathway in dairy cow anterior pituitary cells [J]. International Journal of Molecular Sciences , 2013, 14(11):21474-21488.
[5] 苗培. 催乳素及其受体的研究进展[J]. 中国畜牧兽医文摘, 2016, 32(3):55.
MIAO P. The research progress of prolactin and its receptor [J]. China Animal Husbandry and Veterinary Abstract , 2016, 32(3):55.(in Chinese)
[6] 黄利,李利民,刘之恒,等. 乳癖2号汤对乳腺增生豚鼠雌孕激素水平及病理形态的影响[J]. 四川中医, 2013, 31(1):61-63.
HUANG L, LI L M, LIU Z H, et al. Breast 2 soup for hyperplasia of mammary glands guinea pig female progesterone level and the influence of the pathologic morphology [J]. Journal of Sichuan of Traditional Chinese Medicine , 2013, 31(1):61-63.(in Chinese)
[7] ZOBEL G, LESLIE K. Gradual cessation of milking reduces milk leakage and motivation to be milked in dairy cows at dry-off [J]. Journal of Dairy Science , 2013, 96(8):5064-5071.
[8] TUCKER C B, LACY-HULBERT S J, WEBSTER J R. Effect of milking frequency and feeding level before and after dry off on dairy cattle behavior and udder characteristics[J]. Journal of Dairy Science , 2009, 92(7):3194-3203.
[9] STEYN F J. Nutrient sensing overrides somatostatin and growth hormone-releasing hormone to control pulsatile growth hormone release [J]. Journal of Neuroendocrinology , 2015, 27(7):577-587.
[10] BROWN-BORG H M. Reduced growth hormone signaling and methionine restriction: Interventions that improve metabolic health and extend life span [J]. Annals of the New York Academy of Sciences , 2015, 1363(1):40-49.
[11] FORSYTH I A, WALLIS M. Growth hormone and prolactin-molecular and functional evolution [J]. Journal of Mammary Gland Biology and Neoplasia , 2002, 7(3):291-312.
[12] RHOADS M L, MEYER J P, KOLATH S J, et al. Growth hormone receptor, insulin-like growth factor (IGF)-1, and IGF-binding protein-2 expression in the reproductive tissues of early postpartum dairy cows [J]. Journal of Dairy Science , 2008, 91(5):1802-1813.
[13] MACRINA A L, KAUF A C W, KENSINGER R S. Effect of bovine somatotropin administration during induction of lactation in 15-month-old heifers on production and health [J]. Journal of Dairy Science , 2011, 94(9):4566-4573.
[14] HADI Z, ATASHI H, DADPASAND M, et al. The relationship between growth hormone polymorphism and growth hormone receptor genes with milk yield and reproductive performance in Holstein Dairy Cows [J]. Iranian Journal of Veterinary Research , 2015, 16(3):294-298.
[15] KAWASHIMA C, MUNAKATA M, MATSUI M, et al. Polymorphism in promoter region of growth hormone receptor is associated with potential production capacity of insulin-like growth factor-1 in pre-pubertal Holstein heifers [J]. Journal of Animal Physiology and Animal Nutrition , 2016, 100(6):1037-1040.
[16] LUNDBERG E, KRISTROM B, JONSSON B, et al. Growth hormone (GH) dose-dependent IGF-I response relates to pubertal height gain [J]. BMC Endocrine Disorders , 2015, 15(1):1-14.
[17] RIEHLE C, ABEL E D. Insulin signaling and heart failure [J]. Circulation Research , 2016, 118(7):1151-1169.
[18] AKERS R M. Major advances associated with hormone and growth factor regulation of mammary growth and lactation in dairy cows [J]. Journal of Dairy Science , 2006, 89(4):1222-1234.
[19] 佟慧丽,高学军,李庆章,等. 胰岛素、催乳素对奶山羊乳腺上皮细胞泌乳功能的影响[J]. 畜牧兽医学报, 2008, 39(6):721-725.
TONG H L, GAO X J, LI Q Z, ea al. Impacting of insulin and prolactin on mammary gland epithelial cell line [J]. Chinese Journal of Animal and Veterinary Sciences , 2008, 39(6):721-725.(in Chinese with English abstract)
[20] 陈建晖,佟慧丽,李庆章,等. 胰岛素、催乳素和孕酮对奶牛乳腺上皮细胞泌乳功能的影响[J]. 中国奶牛, 2008(8):9-13.
CHEN J H, DONG H L, LI Q Z, et al. Influence of insulin, prolactin and progesterone on milk-secretion of mammary gland epithelial cell in dairy cow [J]. China Dairy Cattle , 2008, 8: 9-13.(in Chinese with English abstract)
[21] 田青,季昀,庞学燕,等. 胰岛素对奶牛乳腺上皮细胞酪蛋白合成调节机理的研究[J]. 动物营养学报, 2013, 25(3):550-560.
TIAN Q, JI Y, PANG X Y, et al. A study of insulin action mechanism on casein synthesis of bovine mammary epithelial cells [J]. Chinese Journal of Animal Nutrition , 2013, 25(3):550-560.(in Chinese with English abstract)
[22] 李敏. 新型糖皮质激素对肥大细胞脱颗粒的快速作用及其机制研究[D]. 上海:第二军医大学, 2010.
LI M. New glucocorticoid on mast cell degranulation quick effect and its mechanism [D]. Shanghai: Second Military Medical University, 2010.(in Chinese with English abstract)
[23] SHEEHY P A, NICHOLAS K R,WYNN P C. An investigation of the role of insulin in bovine milk protein gene expression in mammary explant culture [J]. Asian Australasian Journal of Animal Sciences , 2000, 13:272-275.
[24] KABOTYANSKI E B, RIJNKELS M, FREEMANZADROWSKI C, et al. Lactogenic hormonal induction of long distance interactions between Β-casein gene regulatory elements [J]. Journal of Biological Chemistry , 2009, 284(34):22815-22824.
[25] LITTLEJOHN M D, HENTY K M, TIPLADYi K, et al. Functionally reciprocal mutations of the prolactin signalling pathway define hairy and slick cattle [J]. Nature Communications , 2014, 5(4):5861-5861.
[26] YANG G, ZHOU J, TENG Y, et al. Mesenchymal TGF-β signaling orchestrates dental epithelial stem cell homeostasis through wnt signaling [J]. Stem Cells , 2014, 32(11):2939-2948.
[27] GRONER B. Transcription factor regulation in mammary epithelial cells [J]. Domestic Animal Endocrinology , 2002, 23(23):25-32.
[28] KLLEINBERG D L, FELDMAN M, RUAN W. IGF-1: An essential factor in terminal end bud formation and ductal morphogenesis [J]. Journal of Mammary Gland Biology & Neoplasia , 2000, 5(1):7-17.
[29] ZARZYNSKA J, GAJKOWSKA B, WOJEWODZKA U, et al. Apoptosis and autophagy in involuting bovine mammary gland is accompanied by up-regulation of TGF-beta1 and suppression of somatotropic pathway [J]. Polish Journal of Veterinary Sciences , 2007, 10(1):1-9.
[30] VRIES L D D, CASEY T, DOVER H, et al. Effects of transforming growth factor-β on mammary remodeling during the dry period of dairy cows [J]. Journal of Dairy Science , 2011, 94(12):6036-6046.
[31] ZHANG Y, MORGAN R, CHEN C, et al. Abstract A120: Tumor-educated B cells acquire LAP/TGF-β1 and PD-L1 expression and suppress antitumor immune response [J]. Cancer Immunology Research , 2016, 4(Suppl. 1):16-19.
[32] NAWATHE A R, CHRISTIAN M, KIM S H, et al. Insulin-like growth factor axis in pregnancies affected by fetal growth disorders [J]. Clinical Epigenetics , 2016, 8(1):1-13.
[33] MURNEY R, STELWAGEN K, WHEELER T T, et al. The effects of milking frequency on insulin-like growth factor 1 signaling within the mammary gland of dairy cows [J]. Journal of Dairy Science , 2015, 98(8):5422-5428.
[34] HEERNANDEZ H, FLORES J A, DELGADILLO J A, et al. Effects of exposure to artificial long days on milk yield, maternal insulin-like growth factor 1 levels and kid growth rate in subtropical goats [J]. Animal Science Journal , 2015, 87(4):484-491.
[35] 李蔚辉,魏学鑫. 糖皮质激素的信号转导系统[J]. 亚太传统医药, 2007, 3(12):21-25.
LI W H, WEI X J. Signal transduction system of glucocorticoid [J]. Asia-Pacific Traditional Medicine , 2007, 3(12):21-25.(in Chinese)
[36] 赵国丽,宫艳斌,韩元,等. 激素和生长因子调控奶牛乳腺发育的研究进展[J]. 中国奶牛, 2011 (6):25-30.
ZHAO G L, GONG Y B, HAN Y, et al. Advances associated with hormone and growth factor regulation of mammary growth and lactation in dairy cows [J]. China Dairy Cattle , 2011 (6):25-30.(in Chinese with English abstract)
[37] WUU W J, LEE C F, HSIN C H, et al. TGF-beta inhibits prolactin-induced expression of beta-casein by a Smad3-dependent mechanism [J]. Journal of Cellular Biochemistry , 2008, 104(5):1647-1659.
[38] SEERRA R, CROWLEY M R. Mouse models of transforming growth factor β impact in breast development and cancer [J]. Endocrine Related Cancer , 2006, 12(4):749-760.

回乳期奶牛血清GH、INS、HC、TGF-β1和IGF-1含量变化

Changes of GH, INS, HC, TGF-β1 and IGF-1 levels in cows’ serum during milk withdrawal period

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