冷鲜鸡污染微生物磺胺类和喹诺酮类耐药基因分析

doi:10.3969/j.issn.1004-1524.2017.04.06

浙江农业学报 ›› 2017, Vol. 29 ›› Issue (4): 555-559.DOI: 10.3969/j.issn.1004-1524.2017.04.06

冷鲜鸡污染微生物磺胺类和喹诺酮类耐药基因分析

何祥祥^{1, 2, 3}, 杨华^{2, 3}, 戴宝玲^{2, 3}, 夏效东¹, 肖英平^{2, 3, *}

1.西北农林科技大学食品科学与工程学院,陕西杨凌 712100;
2.浙江省农业科学院农产品质量标准研究所,浙江杭州 310021;
3.浙江省植物有害生物防控省部共建国家重点实验室培育基地,浙江杭州 310021

收稿日期:2016-11-07 出版日期:2017-04-20 发布日期:2017-04-27
通讯作者: 肖英平,E-mail:ypxiaozju@126.com
作者简介:何祥祥(1991—),男,河南洛阳人,硕士研究生,研究方向为食品科学。E-mail:xianghe2009@126.com
基金资助:
浙江省公益技术运用研究项目(2016C32073); 浙江省植物有害生物防控重点实验室——省部共建国家重点实验室培育基地(2010DS700124-ZM1608)

Detection of the antibiotic genes of sulfonamides and quinolones of bacteria in refrigerated chicken

HE Xiangxiang^{1, 2, 3}, YANG Hua^{2, 3}, DAI Baoling^{2, 3}, XIA Xiaodong¹, XIAO Yingping^{2, 3, *}

1. College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China;
2.Institute of Quality and Standard for Agro-products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;
3. State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2016-11-07 Online:2017-04-20 Published:2017-04-27

摘要/Abstract

摘要： 动物养殖业的发展过程中抗生素的长期使用,导致耐药菌的快速发展。这些耐药菌在动物养殖过程中逐渐被抗生素筛选,在动物性食品生产链中容易被携带传播,对人类食品安全产生很大的威胁。试验选取浙江省绍兴市8个大型超市和11个农贸市场销售的冷鲜鸡为检测对象,采用PCR和测序验证的技术手段,对冷鲜鸡表面微生物中的2种磺胺类耐药基因sulⅠ、sulⅡ和8种喹诺酮类基因qnrA、qnrB、qnrC、qnrD、qnrS、qepA、oqxA与oqxB进行检测。在所检测的样品中,检测出sulⅠ、sulⅡ、qnrD、qnrS、qepA和oqxB基因,检出率分别为100%、100%、100%、89.5%、100%和89.5%。并对所检出的耐药基因进行测序和NCBI收录的耐药基因序列进行Blast比对验证,比对结果同源性分别为100%、99.44%、100%、92.74%、99.82%和100%。试验结果将为冷鲜鸡中耐药基因的风险评估提供有力支持。

关键词: 冷鲜鸡, 细菌, 磺胺类, 喹诺酮类, 耐药基因

Abstract: The long-term use of antibiotics in the animal farming industry has caused rapid expansion of drug resistant bacteria. These drug resistant bacteria are screened during the process of animal farming, and are easily transmitted in the animal production chain, which is a huge threat to human’s food safety. In this study, we aimed at testing the safety of refrigerated chicken collected from eight supermarkets and eleven agricultural markets in Shaoxing city, Zhejiang Province. We detected the antibiotic genes of sulfonamides and quinolones of surface microorganisms of the samples by PCR reaction and sequencing. We detected sul Ⅰ, sulⅡ, qnrD, qnrS, qepA and oqxB in the samples and the detection rate was 100%, 100%, 100%, 89.5%, 100% and 89.5%, respectively. We also sequenced the antibiotic genes with the sequences in NCBI and the coincidence rate was 100%, 99.44%, 100%, 92.74%, 99.82% and 100%, respectively. This study will provide effective support for risk assessment of antibiotic genes in the refrigerated chicken.

Key words: refrigerated chicken, bacteria, sulfonamides, quinolones, drug-resistance genes

中图分类号:

S879.2

何祥祥, 杨华, 戴宝玲, 夏效东, 肖英平. 冷鲜鸡污染微生物磺胺类和喹诺酮类耐药基因分析[J]. 浙江农业学报, 2017, 29(4): 555-559.

HE Xiangxiang, YANG Hua, DAI Baoling, XIA Xiaodong, XIAO Yingping. Detection of the antibiotic genes of sulfonamides and quinolones of bacteria in refrigerated chicken[J]. , 2017, 29(4): 555-559.

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冷鲜鸡污染微生物磺胺类和喹诺酮类耐药基因分析

Detection of the antibiotic genes of sulfonamides and quinolones of bacteria in refrigerated chicken

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