防控温郁金细菌性枯萎病的生物有机肥研制与应用

doi:10.3969/j.issn.1004-1524.2023.03.16

浙江农业学报 ›› 2023, Vol. 35 ›› Issue (3): 630-638.DOI: 10.3969/j.issn.1004-1524.2023.03.16

防控温郁金细菌性枯萎病的生物有机肥研制与应用

冯林(), 周铨, 陈卫良, 毛碧增^*()

浙江大学生物技术研究所,农业农村部作物病虫分子生物学重点实验室,浙江省作物病虫生物学重点实验室,浙江杭州 310058

收稿日期:2022-04-22 出版日期:2023-03-25 发布日期:2023-04-07
通讯作者: ^*毛碧增,E-mail:maobz@zju.edu.cn
作者简介:冯林(1994—),男,云南昆明人,硕士研究生,研究方向为温郁金细菌性枯萎病的综合防治。E-mail:21716411@zju.edu.cn
基金资助:
农业农村部农业重大技术协同推广项目(2020XTTGZYC02);浙江省“三农六方”科技协作计划(CTZB-F160728AWZ-SNY1-37)

Development and application of bio-organic fertilizers for prevention and control of bacterial wilt of Curcuma wenyujin

FENG Lin(), ZHOU Quan, CHEN Weiliang, MAO Bizeng^*()

Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province, Ministry of Agriculture and Rural Affairs Key Lab of Molecular Biology of Crop Pathogens and Insects, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China

Received:2022-04-22 Online:2023-03-25 Published:2023-04-07

摘要/Abstract

摘要：

茄科劳尔氏菌(Ralstonia solanaceraum)引起的温郁金(Curcuma wenyujin Y.H.Chen et C.Ling)细菌性枯萎病是目前温郁金种植中的毁灭性病害。本文利用2株高效拮抗菌Paenibacillus jamila ZJU-C612-3和Bacillus amyloliquefaciens ZJU-C612-4研制生物有机肥,并进行田间防治效果试验。温室盆栽试验结果显示,施生物有机肥的温郁金发病率下降了60百分点;田间试验显示,每667 m²施200 kg生物有机肥作为基肥的田块中,健康根茎的发病率降低17.66百分点,且植株生长健壮。此外,施生物有机肥能缓解温郁金的连作障碍,在5 a连作田块施该肥后,在病害高发期的7月和8月,连作根茎的发病率分别下降9.43百分点和19.75百分点。研究结果可为今后防治温郁金细菌性枯萎病的专用生物有机肥的产业化开发提供依据。

关键词: 温郁金, 茄科劳尔氏菌, 生物有机肥, 细菌性枯萎病, 发病率

Abstract:

Bacterial wilt of Curcuma wenyujin (BWCW) caused by Ralstonia solanaceraum was becoming a common destructive disease in the planting of C. wenyujin at present. In order to control the disease, a bio-organic fertilizer (BOF) was developed by using Paenibacillus jamila ZJU-C612-3 and Bacillus amyloliquefaciens ZJU-C612-4, which were screened early in our laboratory and showed strong antagonistic activities to R. solanaceraum. The incidence rate of C. wenyujin tuber treated with BOF in greenhouse pot culture was reduced by 60 percentage point. After using 200 kg BOF in 667 m² field, the incidence rate of healthy C. wenyujin tuber was reduced by 17.66 percentage point, and the plants grew well. In addition, the BOF could alleviate the monocropping obstacle of C. wenyujin. After applying bio-organic fertilizer in the 5-year continuous cropping field, the incidence rate of continuous cropping C. wenyujin tuber decreased by 9.43 percentage point and 19.75 percentage point in July and August. This study result could provide a basis for the industrialized development of bio-organic fertilizer for the control of bacterial wilt of C. wenyujin in future.

Key words: Curcuma wenyujin, bacterial wilt, bio-organic fertilizer, bacterial wilt, incidence rate

中图分类号:

S476

冯林, 周铨, 陈卫良, 毛碧增. 防控温郁金细菌性枯萎病的生物有机肥研制与应用[J]. 浙江农业学报, 2023, 35(3): 630-638.

FENG Lin, ZHOU Quan, CHEN Weiliang, MAO Bizeng. Development and application of bio-organic fertilizers for prevention and control of bacterial wilt of Curcuma wenyujin[J]. Acta Agriculturae Zhejiangensis, 2023, 35(3): 630-638.

图/表 10

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质荷比 Mass-to- charge ratio	离子型 Ionic type	化合物 Compound	P. jamilae ZJU-C612-3
883.56	[M+H]+	C15 Fusaricidin A	+
897.58	[M+H]+	C15 Fusaricidin B	+
911.59	[M+H]+	C15 Fusaricidin E	+
925.61	[M+H]+	C15 Fusaricidin F	+
947.56	[M+H]+	C15 Fusaricidin C	+
961.57	[M+H]+	C15 Fusaricidin D	+
975.59	[M+H]+	C15 Fusaricidin G	+
931.56	[M+H]+	C15 Fusaricidin H	+
945.58	[M+H]+	C15 Fusaricidin I	+
959.90	[M+H]+	C15 Fusaricidin J	+
939.62	[M+H]+	C17 Fusaricidin E	+
953.64	[M+H]+	C17 Fusaricidin F	—
957.59	[M+H]+	C17 Fusaricidin C	—
989.60	[M+H]+	C17 Fusaricidin D	+
1003.62	[M+H]+	C17 Fusaricidin G	—
973.61	[M+H]+	C15 Fusaricidin I	+
987.62	[M+H]+	C15 Fusaricidin J	—
981.67	[M+H]+	C18 Fusaricidin F	—
1 017.63	[M+H]+	C18 Fusaricidin D	—
1 031.65	[M+H]+	C18 Fusaricidin G	—
1 001.64	[M+H]+	C18 Fusaricidin I	+
1015.65	[M+H]+	C18 Fusaricidin J	—
919.8	[M+Na]+	Fusaricidin B	+
935.8	[M+K]+	Fusaricidin B	+
961.9	[M+H]+	FusaricidinD	+
968.9	[M+H]+	NI	+
1 191.9	[M+H]+	C9 Polymyxin P	+
1 213.9	[M+Na]+	C9 Polymyxin P	—
1 203.9	[M+H]+	C9 Polymyxin B	—
1 225.9	[M+Na]+	C9 Polymyxin B	—

质荷比 Mass-to- charge ratio	离子型 Ionic type	化合物 Compound	P. jamilae ZJU-C612-3
883.56	[M+H]+	C15 Fusaricidin A	+
897.58	[M+H]+	C15 Fusaricidin B	+
911.59	[M+H]+	C15 Fusaricidin E	+
925.61	[M+H]+	C15 Fusaricidin F	+
947.56	[M+H]+	C15 Fusaricidin C	+
961.57	[M+H]+	C15 Fusaricidin D	+
975.59	[M+H]+	C15 Fusaricidin G	+
931.56	[M+H]+	C15 Fusaricidin H	+
945.58	[M+H]+	C15 Fusaricidin I	+
959.90	[M+H]+	C15 Fusaricidin J	+
939.62	[M+H]+	C17 Fusaricidin E	+
953.64	[M+H]+	C17 Fusaricidin F	—
957.59	[M+H]+	C17 Fusaricidin C	—
989.60	[M+H]+	C17 Fusaricidin D	+
1003.62	[M+H]+	C17 Fusaricidin G	—
973.61	[M+H]+	C15 Fusaricidin I	+
987.62	[M+H]+	C15 Fusaricidin J	—
981.67	[M+H]+	C18 Fusaricidin F	—
1 017.63	[M+H]+	C18 Fusaricidin D	—
1 031.65	[M+H]+	C18 Fusaricidin G	—
1 001.64	[M+H]+	C18 Fusaricidin I	+
1015.65	[M+H]+	C18 Fusaricidin J	—
919.8	[M+Na]+	Fusaricidin B	+
935.8	[M+K]+	Fusaricidin B	+
961.9	[M+H]+	FusaricidinD	+
968.9	[M+H]+	NI	+
1 191.9	[M+H]+	C9 Polymyxin P	+
1 213.9	[M+Na]+	C9 Polymyxin P	—
1 203.9	[M+H]+	C9 Polymyxin B	—
1 225.9	[M+Na]+	C9 Polymyxin B	—

质荷比 Mass-to- charge ratio	离子型 Ionic type	化合物 Compound	B. amyloli- quefaciens ZJU-C612-4
1 045.693	[M+K]+	C13 Surfactin	—
1 060.793	[M+K]+	C14 Surfactin	+
1 069.694	[M+K]+	C14 Bacillomycin D	+
1 075.861	[M+K]+	C15 Surfactin	+
1 083.742	[M+K]+	C15 Bacillomycin D	+
1 097.788	[M+K]+	C16 Bacillomycin D	+
1 109.733	[M+K]+	C16 Bacillomycin F	+
1 436.194	[M+H]+	C14 Fengycin	—
1 466.251	NI	NI	+
1 478.274	[M+H]+	C17 Fengycin A	+
1 502.230	[M+K]+	C16 Fengycin A	+
1 516.267	[M+K]+	C17 Fengycin A	+
1 529.8	[M+K]+	C18 Fengycin A	+
1 557.8	[M+K]+	C20 Fengycin A	+

质荷比 Mass-to- charge ratio	离子型 Ionic type	化合物 Compound	B. amyloli- quefaciens ZJU-C612-4
1 045.693	[M+K]+	C13 Surfactin	—
1 060.793	[M+K]+	C14 Surfactin	+
1 069.694	[M+K]+	C14 Bacillomycin D	+
1 075.861	[M+K]+	C15 Surfactin	+
1 083.742	[M+K]+	C15 Bacillomycin D	+
1 097.788	[M+K]+	C16 Bacillomycin D	+
1 109.733	[M+K]+	C16 Bacillomycin F	+
1 436.194	[M+H]+	C14 Fengycin	—
1 466.251	NI	NI	+
1 478.274	[M+H]+	C17 Fengycin A	+
1 502.230	[M+K]+	C16 Fengycin A	+
1 516.267	[M+K]+	C17 Fengycin A	+
1 529.8	[M+K]+	C18 Fengycin A	+
1 557.8	[M+K]+	C20 Fengycin A	+

项目名称 Entry name	NY884—2012规定指标 NY884—2012 specified indicators	NY525—2012规定指标 NY525—2012 specified indicators	生物有机肥实测指标 Bio organic fertilizer index
有效活菌数Effective viable count/(10⁸ g^-1)	≥0.2	—	10
有机质(以烘干计)Organic matter/%	≥40	≥45	54.40
总养分(N+P₂O₅+K₂O) Total nutrient/%	—	≥5	2.40+3.28+1.78
水分Water content/%	≤30	≤30	19.30
pH	5.5~8.5	5.5~8.5	6.9
总砷(以干基计)As/(mg·kg^-1)	≤15	≤15	3.40
总汞(以干基计)Hg/(mg·kg^-1)	≤2	≤2	0.10
总铅(以干基计)Pb/(mg·kg^-1)	≤50	≤50	12.20
总镉(以干基计)Cd/(mg·kg^-1)	≤3	≤3	0.30
总铬(以干基计)Cr/(mg·kg^-1)	≤150	≤150	57.60
粪大肠埃希菌群Fecal coliform/g^-1	≤100	≤100	<0.3
蛔虫卵死亡率Ascaris egg mortality/%	≥95	≥95	100.00

防控温郁金细菌性枯萎病的生物有机肥研制与应用

Development and application of bio-organic fertilizers for prevention and control of bacterial wilt of Curcuma wenyujin

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处理 Treatment	发病率 Incidence rate/%	生长状况 Growth status
1	100 a	所有植株枯萎死亡All plants wither and die
2	66.70±6.67 b	存活植株长势较差,叶片褪绿,叶缘枯黄
		The surviving plants grew poorly, leaf edges were withered and yellow
3	40.00±6.67 c	存活植株长势较为健壮,叶片未褪色The surviving plants grew well and the leaves were not faded
4	66.70±13.33 b	存活植株长势较差,叶片褪绿The surviving plants grew poorly and their leaves faded

处理 Treatment	处理方式 Treatment method	平均单株产量 Average yield per plant/kg
1	常规根茎+复合肥 Conventional tuber+compound fertilizer	0.60±0.32
2	连作根茎+复合肥 Continuous cropping tuber+ compound fertilizer	0.37±0.16
3	常规根茎+BOF Conventional tuber+BOF	1.16±0.41
4	连作根茎+BOF Continuous cropping tuber+BOF	0.71±0.29

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