锌指蛋白转录因子Di19参与调控大豆干旱响应的研究进展

doi:10.3969/j.issn.1004-1524.2020.02.22

浙江农业学报 ›› 2020, Vol. 32 ›› Issue (2): 373-382.DOI: 10.3969/j.issn.1004-1524.2020.02.22

• 综述 • 上一篇

锌指蛋白转录因子Di19参与调控大豆干旱响应的研究进展

张古文¹, 沈立², 郑华章³, 刘娜¹, 冯志娟¹, 龚亚明^1,*

1.浙江省农业科学院蔬菜研究所,浙江杭州310021;
2.浙江勿忘农种业股份有限公司,浙江杭州310020;
3.余姚市农业技术推广服务总站,浙江余姚315400

收稿日期:2019-08-29 出版日期:2020-02-25 发布日期:2020-03-13
通讯作者: *龚亚明,E-mail:gongym07@126.com
作者简介:张古文(1981—),男,山西运城人,博士,主要从事菜用大豆抗逆及品质机理研究。E-mail:zhangguwen@126.com
基金资助:
国家重点研发计划(2017YFD01011500); 浙江省自然科学基金(LY17C150007); 浙江省农业新品种选育重大科技专项(2016C02051-7-1); 浙江省植物有害生物防控重点实验室—省部共建国家重点实验室培育基地项目(2010DS700124-KF1611); 浙江省农业科学院地方合作项目(CA20170011); 农业农村部物种品种资源保护项目暨第三次全国作物种质资源普查与收集行动(111721301354052231)

Research advances of zinc finger protein transcription factor Di19 in regulation of soybean responding to drought stress

ZHANG Guwen¹, SHEN Li², ZHENG Huazhang³, LIU Na¹, FENG Zhijuan¹, GONG Yaming^1,*

1. Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;
2. Zhejiang Wuwangnong Seeds Shareholding Co., Ltd., Hangzhou 310020, China;
3. Yuyao Agricultural Technology Extension Service Station, Yuyao 315400, China

Received:2019-08-29 Online:2020-02-25 Published:2020-03-13

摘要/Abstract

摘要： 干旱是人类面临的最主要自然灾害之一,是影响农作物生产最主要的环境因素。锌指蛋白指含有锌离子、具有手指状结构域的一类蛋白质,是植物中发现种类最多、调控作用最显著的一类转录因子,在植物干旱响应中发挥重要作用。Di19是最新发现的一类小分子锌指蛋白,其含有两个C2H2型锌指结构域,受干旱和高盐诱导,在植物的抗旱响应中起积极作用。大豆根系不发达,需水量多,对干旱敏感,水分亏缺是制约其产量提高和品质提升的最重要环境因子。本文评述了大豆耐旱性研究现状,锌指蛋白及其家族最新成员Di19在大豆耐旱性响应过程中发挥的作用,以期为大豆耐旱性改良提供参考。

关键词: 锌指蛋白, 转录因子, Di19, 大豆, 干旱响应

Abstract: Drought is one of the most serious natural disasters faced by human beings, and also the most important environmental factor affecting crop production. Zinc finger protein refers to a class of proteins containing zinc ion and finger-like domains. It is the most common type of transcription factor found in plants and plays the most important role in plant drought response. Di19 is a newly discovered small zinc finger protein containing two C2H2 type zinc finger domains, which is induced by drought and high salt, and plays an active role in plant drought response. Soybean roots are underdeveloped, require more water, sensitive to drought, and water deficit is the most important environmental factor that restricts their yield and quality. In this paper, the research status of soybean drought tolerance, the role of zinc finger protein and its newest member Di19 in drought tolerance response of soybean were reviewed in order to provide reference for drought tolerance improvement of soybean.

Key words: zinc finger protein, transcription factor, Di19 (Drought induce 19), soybean, drought stress

中图分类号:

S565.1

张古文, 沈立, 郑华章, 刘娜, 冯志娟, 龚亚明. 锌指蛋白转录因子Di19参与调控大豆干旱响应的研究进展[J]. 浙江农业学报, 2020, 32(2): 373-382.

ZHANG Guwen, SHEN Li, ZHENG Huazhang, LIU Na, FENG Zhijuan, GONG Yaming. Research advances of zinc finger protein transcription factor Di19 in regulation of soybean responding to drought stress[J]. , 2020, 32(2): 373-382.

参考文献

[1] 原向阳, 郭平毅, 张丽光, 等. 干旱胁迫下草甘膦对抗草甘膦大豆幼苗保护酶活性及脂质过氧化作用的影响[J]. 中国农业科学, 2010, 43(4): 698-705.
YUAN X Y, GUO P Y, ZHANG L G, et al.Glyphosate and post-drought rewatering on protective enzyme activities and membrane lipid peroxidation in leaves of glyphosate-resistant soybean [Glycine amx (L.) Merr.]seedlings[J]. Scientia Agricultura Sinica, 2010, 43(4): 698-705. (in Chinese with English abstract)
[2] 张永芳, 王润梅, 张东旭, 等. 我国大豆耐旱性研究进展[J]. 山西农业科学, 2011, 39(1): 88-90.
ZHANG Y F, WANG R M, ZHANG D X, et al.Research progress in drought resistance of soybean(Glycine max) in China[J]. Journal of Shanxi Agricultural Sciences, 2011, 39(1): 88-90.(in Chinese with English abstract)
[3] 康蕾, 张红旗. 我国五大粮食主产区农业干旱态势综合研究[J]. 中国生态农业学报, 2014, 22(8): 928-937.
KANG L, ZHANG H Q.Comprehensive research on the state of agricultural drought in five main grain producing areas in China[J]. Chinese Journal of Eco-Agriculture, 2014, 22(8): 928-937.(in Chinese with English abstract)
[4] 莫金钢, 马建, 张丽辉, 等. 干旱胁迫对大豆种子萌发的影响[J]. 大豆科学, 2014, 33(5): 701-704.
MO J G, MA J, ZHANG L H, et al.Effect of drought stress on germination of soybean[J]. Soybean Science, 2014, 33(5): 701-704.(in Chinese with English abstract)
[5] 王国夫. 不同菜用大豆品种发芽期耐旱性鉴定[J]. 绍兴文理学院学报(自然科学), 2015, 35(1): 14-18.
WANG G F.Identification of drought-tolerance of soybean cultivars at germination growth stages[J]. Journal of Shaoxing University(Natural Science), 2015, 35(1): 14-18.(in Chinese with English abstract)
[6] MILLA M A R, TOWNSEND J, CHANG I, et al. The Arabidopsis AtDi19 gene family encodes a novel type of Cys2/His2 zinc-finger protein implicated in ABA-independent dehydration, high-salinity stress and light signaling pathways[J]. Plant Molecular Biology, 2006, 61(1/2): 13-30.
[7] LI G, TAI F J, ZHENG Y, et al.Two cotton Cys2/His2-type zinc-finger proteins, GhDi19-1 and GhDi19-2, are involved in plant response to salt/drought stress and abscisic acid signaling[J]. Plant Molecular Biology, 2010, 74(4/5): 437-452.
[8] LIU W X, ZHANG F C, ZHANG W Z, et al.Arabidopsis Di19 functions as a transcription factor and modulates PR1, PR2, and PR5 expression in response to drought stress[J]. Molecular Plant, 2013, 6(5): 1487-1502.
[9] QIN L X, LI Y, LI D D, et al.Arabidopsis drought-induced protein Di19-3 participates in plant response to drought and high salinity stresses[J]. Plant Molecular Biology, 2014, 86(6): 609-625.
[10] SAPRA V T, ANAELE A O.Screening soybean genotypes for drought and heat tolerance[J]. Journal of Agronomy and Crop Science, 1991, 167(2): 96-102.
[11] 谢甫绨, 董钻, 孙艳环, 等. 不同生育时期干旱对大豆生长和产量的影响[J]. 沈阳农业大学学报, 1994, 25(1): 13-16.
XIE F T, DONG Z, SUN Y H, et al.Influence of drought on growth and yield of soybeans at different growth stages[J]. Journal of Shengyang Agricultural University, 1994, 25(1): 13-16.(in Chinese with English abstract)
[12] 吴旭红, 王玉梅, 张百忱. 不同品种大豆幼苗抗旱性的研究初报[J]. 齐齐哈尔师范学院院报(自然科学版), 1996, 16(4): 55-58.
WU X H, WANG Y M, ZHANG B C.A preliminary study on the drought resistance of soybean seedling of different varieties[J]. Journal of Qiqihar teachers’ college (Natural Science), 1996, 16(4): 55-58. (in Chinese with English abstract)
[13] ZHANG Y Z, HAN Y H.Effect of high temperature and/or drought stress on the activities of SOD and POD of intact leaves in two soybean (G. max) cultivars[J]. Soybean Genetics Newsletter, 1997, 24: 39-40.
[14] SAKAMOTO H, MARUYAMA K, SAKUMA Y, et al.Arabidopsis Cys2/His2-type zinc-finger proteins function as transcription repressors under drought, cold, and high-salinity stress conditions[J]. Plant Physiology, 2004, 136(1): 2734-2746.
[15] 王启明, 徐心诚, 吴诗光, 等. 干旱胁迫对不同大豆品种苗期叶片渗透调节物质含量和细胞膜透性的影响[J]. 种子, 2005, 24(8): 9-12.
WANG Q M, XU X C, WU S G, et al.The effect of drought stress on the content of osmotic adjusting substance in leaves and cell membrance permeability of different soybean varieties in seedling period[J]. Seed, 2005, 24(8): 9-12.(in Chinese with English abstract)
[16] THU N B A, HOANG X L T, NGUYEN T D H, et al. Differential expression of two-component system-related drought-responsive genes in two contrasting drought-tolerant soybean cultivars DT51 and MTD720 under well-watered and drought conditions[J]. Plant Molecular Biology Reporter, 2015, 33(5): 1599-1610.
[17] ZHOU Q, WU Y Y, ZHENG C L, et al.Triadimefon induced C and N metabolism and root ultra-structural changes for drought stress protection in soybean at flowering stage[J]. Journal of Plant Growth Regulation, 2016, 35(1): 222-231.
[18] MIAN M A R, BAILEY M A, ASHLEY D A, et al. Molecular markers associated with water use efficiency and leaf ash in soybean[J]. Crop Science, 1996, 36(5): 1252.
[19] MIAN M A R, ASHLEY D A, BOERMA H R. An additional QTL for water use efficiency in soybean[J]. Crop Science, 1998, 38(2): 390.
[20] 刘莹. 大豆根区逆境耐性的鉴定和相关根系性状的遗传分析与QTL定位[D]. 南京: 南京农业大学, 2005.
LIU Y.Identification of tolerance to rhizosperical stresses and inheretance and QTL locatting of related root traits in soybean (Glycine max(L.) Merr.)[D]. Nanjing: Nanjing Agricultural University, 2005.(in Chinese with English abstract)
[21] DU W J, YU D Y, FU S X.Analysis of QTLs for the trichome density on the upper and downer surface of leaf blade in soybean [Glycine max(L.) Merr.][J]. Agricultural Sciences in China, 2009, 8(5): 529-537.
[22] 李灿东, 蒋洪蔚, 刘春燕, 等. 大豆耐旱选择群体QTL定位[J]. 作物学报, 2011, 37(4): 603-611.
LI C D, JIANG H W, LIU C Y, et al.QTL identification of drought tolerance to soybean in selection population[J]. Acta Agronomica Sinica, 2011, 37(4): 603-611.(in Chinese with English abstract)
[23] ABDEL-HALEEM H, CARTER T E, PURCELL L C, et al.Mapping of quantitative trait loci for canopy-wilting trait in soybean (Glycine max L. Merr.)[J]. Theoretical and Applied Genetics, 2012, 125(5): 837-846.
[24] 邢光南, 刘泽稀楠, 谭连美, 等. 大豆叶面茸毛密度和长度的QTL定位[J]. 作物学报, 2013, 39(1): 12-20.
XING G N, LIU Z, TAN L M, et al.QTL mapping of pubescence density and length on leaf surface of soybean[J]. Acta Agronomica Sinica, 2013, 39(1): 12-20.(in Chinese with English abstract)
[25] ZHANG D, ZHANG H Y, CHU S S, et al.Integrating QTL mapping and transcriptomics identifies candidate genes underlying QTLs associated with soybean tolerance to low-phosphorus stress[J]. Plant Molecular Biology, 2017, 93(1/2): 137-150.
[26] 张大勇, 易金鑫, 何晓兰, 等. 大豆水通道蛋白家族基因的相关研究[C]//江苏省遗传学会会员代表大会暨学术研讨会, 2010.
[27] FARIA J A, REIS P A, REIS M T, et al.The NAC domain-containing protein, GmNAC6, is a downstream component of the ER stress-and osmotic stress-induced NRP-mediated cell-death signaling pathway[J]. Plant Signaling & Behavior, 2012, 11(1): 129
[28] LE D T, NISHIYAMA R, WATANABE Y, et al.Differential gene expression in soybean leaf tissues at late developmental stages under drought stress revealed by genome-wide transcriptome analysis[J]. PLoS One, 2012, 7(11): e49522.
[29] VIDAL R O, DO NASCIMENTO L C, MAURÍCIO COSTA MONDEGO J, et al. Identification of SNPs in RNA-seq data of two cultivars of Glycine max(soybean) differing in drought resistance[J]. Genetics and Molecular Biology, 2012, 35(1 suppl 1): 331-334.
[30] XU J Y, XUE C C, XUE D, et al.Overexpression of GmHsp90s, a heat shock protein 90 (Hsp90) gene family cloning from soybean, decrease damage of abiotic stresses in Arabidopsis thaliana[J]. PLoS One, 2013, 8(7): e69810.
[31] LUO X, BAI X, SUN X L, et al.Expression of wild soybean WRKY20 in Arabidopsis enhances drought tolerance and regulates ABA signalling[J]. Journal of Experimental Botany, 2013, 64(8): 2155-2169.
[32] 秦迪, 赵翠兰, 郑成忠, 等. 转BADH基因大豆耐旱性分析[J]. 中国油料作物学报, 2015, 37(6): 752-758.
QIN D, ZHAO C L, ZHENG C Z, et al.Drought tolerance of transgenic soybean with BADH gene[J]. Chinese Journal of Oil Crop Sciences, 2015, 37(6): 752-758.(in Chinese with English abstract)
[33] SINGH K.Transcription factors in plant defense and stress responses[J]. Current Opinion in Plant Biology, 2002, 5(5): 430-436.
[34] TRAN L S P, NAKASHIMA K, SAKUMA Y, et al. Isolation and functional analysis of Arabidopsis stress-inducible NAC transcription factors that bind to a drought-responsive Cis-element in the early responsive to dehydration stress 1 promoter[J]. The Plant Cell, 2004, 16(9): 2481-2498.
[35] JOO J, LEE Y H, SONG S I.Overexpression of the rice basic leucine zipper transcription factor OsbZIP12 confers drought tolerance to rice and makes seedlings hypersensitive to ABA[J]. Plant Biotechnology Reports, 2014, 8(6): 431-441.
[36] ENGLBRECHT C C, SCHOOF H, BÖHM S. Conservation, diversification and expansion of C2H2 zinc finger proteins in the Arabidopsis thaliana genome[J]. BMC Genomics, 2004, 5(1): 39.
[37] 田路明, 黄丛林, 张秀海, 等. 逆境相关植物锌指蛋白的研究进展[J]. 生物技术通报, 2005(6): 12-16.
TIAN L M, HUANG C L, ZHANG X H, et al.Advances of plant zinc finger proteins involved in abiotic stress[J]. Biotechnology Information, 2005(6): 12-16.(in Chinese with English abstract)
[38] PABO C O, PEISACH E, GRANT R A.Design and selection of novel Cys2His2 zinc finger proteins[J]. Annual Review of Biochemistry, 2001, 70(1): 313-340.
[39] SUGANO S, KAMINAKA H, RYBKA Z, et al.Stress-responsive zinc finger gene ZPT2-3 plays a role in drought tolerance in Petunia[J]. The Plant Journal, 2003, 36(6): 830-841.
[40] HUANG X Y, CHAO D Y, GAO J P, et al.A previously unknown zinc finger protein, DST, regulates drought and salt tolerance in rice via stomatal aperture control[J]. Genes & Development, 2009, 23(15): 1805-1817.
[41] KIM J C, LEE S H, CHEONG Y H, et al.A novel cold-inducible zinc finger protein from soybean, SCOF-1, enhances cold tolerance in transgenic plants[J]. The Plant Journal, 2001, 25(3): 247-259.
[42] 刘萌萌. 大豆C2H2型锌指蛋白转录因子基因的克隆与鉴定[D]. 北京: 中国农业科学院, 2007.
LIU M M.Isolation and characterization of C2H2 type zinc finger transcription factor in Glycine max(L.) Merrill[D]. Beijing: Chinese Academy of Agricultural Sciences, 2007.(in Chinese with English abstract)
[43] 白晶, 张必弦, 李新玲, 等. 野生大豆(Glycine soja) C2H2型锌指蛋白基因的克隆与序列分析[J]. 大豆科学, 2009, 28(1): 21-25.
BAI J, ZHANG B X, LI X L, et al.Cloning and sequence analysis of a C2H2 type zinc finger protein gene from Glycine soja[J]. Soybean Science, 2009, 28(1): 21-25. (in Chinese with English abstract)
[44] 吴学闯, 曹新有, 陈明, 等. 大豆C3HC4型RING锌指蛋白基因GmRZFP1克隆与表达分析[J]. 植物遗传资源学报, 2010, 11(3): 343-348.
WU X C, CAO X Y, CHEN M, et al.Isolation and expression pattern assay of a C3HC4-type RING zinc finger protein gene GmRZFP1 in Glycine max(L.)[J]. Journal of Plant Genetic Resources, 2010, 11(3): 343-348.(in Chinese with English abstract)
[45] 单曙光, 于国红, 徐娜, 等. 大豆转录因子GmC2H2基因转化拟南芥效果分析[J]. 生物技术通报, 2011(12): 75-81.
SHAN S G, YU G H, XU N, et al.Effect analysis of transcription factor GmC2H2 in transgenic Arabidopsis[J]. Biotechnology Bulletin, 2011(12): 75-81. (in Chinese with English abstract)
[46] 韩丹, 王丕武, 曲静, 等. 大豆C2H2型锌指蛋白基因SCTF-1转化及功能分析[J]. 中国油料作物学报, 2016, 38(3): 307-312.
HAN D, WANG P W, QU J, et al.Transformation and functional analysis of soybean SCTF-1 gene encoding a C2H2-typezinc finger protein[J]. Chinese Journal of Oil Crop Sciences, 2016, 38(3): 307-312. (in Chinese with English abstract)
[47] KANG X J, CHONG J, NI M.HYPERSENSITIVE TO RED AND BLUE 1, a ZZ-type zinc finger protein, regulates phytochrome B-mediated red and cryptochrome-mediated blue light responses[J]. The Plant Cell, 2005, 17(3): 822-835.
[48] SEARLES M A, LU D, KLUG A.The role of the central zinc fingers of transcription factor IIIA in binding to 5 S RNA1[J]. Journal of Molecular Biology, 2000, 301(1): 47-60.
[49] WOLFE S A, NEKLUDOVA L, PABO C O.DNA recognition by Cys2His2 zinc finger proteins[J]. Annual Review of Biophysics and Biomolecular Structure, 2000, 29(1): 183-212.
[50] FUKAMATSU Y, MITSUI S, YASUHARA M, et al.Identification of LOV KELCH PROTEIN₂ (LKP2)-interacting factors that can recruit LKP2 to nuclear bodies[J]. Plant and Cell Physiology, 2005, 46(8): 1340-1349.
[51] JEANNETTE E, RONA J, BARDAT F, et al.Induction of RAB18 gene expression and activation of K+ outward rectifying channels depend on an extracellular perception of ABA in Arabidopsis thaliana suspension cells[J]. The Plant Journal, 1999, 18(1): 13-22.
[52] KARIOLA T, BRADER G, HELENIUS E, et al.EARLY RESPONSIVE TO DEHYDRATION 15, a negative regulator of abscisic acid responses in Arabidopsis[J]. Plant Physiology, 2006, 142(4): 1559-1573.
[53] LI S, XU C H, YANG Y N, et al.Functional analysis of TaDi19A, a salt-responsive gene in wheat[J]. Plant Cell & Environment, 2010, 33: 117-129.
[54] 李朔. 小麦耐盐渐渗系山融3号根系盐胁迫转录组分析及相关基因功能研究[D]. 济南: 山东大学, 2009.
LI S.Expression profiles of wheat somatic hybrid introgression line Shanrong No.3 under salt stress and functional analysis of salt responsive genes[D]. Jinan: Shandong University, 2009.(in Chinese with English abstract)
[55] 茹京娜, 于太飞, 陈隽, 等. 小麦锌指转录因子TaDi19A对低温的响应及其互作蛋白的筛选[J]. 中国农业科学, 2017, 50(13): 2411-2422.
RU J N, YU T F, CHEN J, et al.Response of wheat zinc-finger transcription factor TaDi19A to cold and its screening of interacting proteins[J]. Scientia Agricultura Sinica, 2017, 50(13): 2411-2422.(in Chinese with English abstract)
[56] 赵娟莹, 刘佳明, 冯志娟, 等. 大豆锌指转录因子GmDi19-5对高温的响应及互作蛋白的筛选[J]. 中国农业科学, 2017, 50(12): 2389-2398.
ZHAO J Y, LIU J M, FENG Z J, et al.The response to heat and screening of the interacting proteins of zinc finger protein GmDi19-5 in soybean[J]. Scientia Agricultura Sinica, 2017, 50(12): 2389-2398.(in Chinese with English abstract)
[57] 张敏. 玉米抗逆基因ZmDi19-5的功能研究[D]. 合肥: 安徽农业大学, 2017.
ZHANG M.Functional analysis of a stress resistant gene ZmDi19-5 from maize[D]. Hefei: Anhui Agricultural University, 2017.(in Chinese with English abstract)
[58] 张新宇, 林书岱, 张涛, 等. 棉花C2H2类型锌指蛋白基因GhSIZ1的克隆及表达分析[J]. 棉花学报, 2015, 27(3): 189-197.
ZHANG X Y, LIN S D, ZHANG T, et al.Cloning and expression analysis of GhSIZ1, encoding a C2H2 zinc finger protein in cotton(Gossypium hirsutum)[J]. Cotton Science, 2015, 27(3): 189-197.(in Chinese with English abstract)
[59] 李灿东, 苗兴奋, 蒋洪蔚, 等. 大豆耐旱选择群体叶片持水能力QTL定位[J]. 中国农学通报, 2011, 27(9): 152-155.
LI C D, MIAO X F, JIANG H W, et al.QTL identification of WRC to soybean in drought tolerance selection population[J]. Chinese Agricultural Science Bulletin, 2011, 27(9): 152-155.(in Chinese with English abstract)

编辑推荐

Metrics

阅读次数

全文

1796

HTML			PDF

最新录用	在线预览	正式出版	最新录用	在线预览	正式出版
0	0	4	0	0	1792

来源	本网站	其他网站

次数	1231	565
比例	69%	31%

摘要

1341

最新录用	在线预览	正式出版

0	0	1341

	来源	本网站

	次数	1341
	比例	100%

锌指蛋白转录因子Di19参与调控大豆干旱响应的研究进展

Research advances of zinc finger protein transcription factor Di19 in regulation of soybean responding to drought stress

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	何佳琦, 翟莹, 张军, 邱爽, 李铭杨, 赵艳, 张梅娟, 马天意. 大豆转录因子GmDof1.5的克隆与非生物胁迫诱导表达[J]. 浙江农业学报, 2021, 33(1): 1-7.
[2]	杨乙未, 肖华, 陈浒, 肖聶佳, 郭城. 喀斯特地区不同玫瑰混农林模式的土壤螨类群落结构特征[J]. 浙江农业学报, 2021, 33(1): 112-121.
[3]	邱文怡, 王诗雨, 李晓芳, 徐恒, 张华, 朱英, 王良超. MYB转录因子参与植物非生物胁迫响应与植物激素应答的研究进展[J]. 浙江农业学报, 2020, 32(7): 1317-1328.
[4]	李君霞, 王春义, 丁宇涛, 代书桃, 朱灿灿, 宋迎辉, 秦娜, 陈宇翔. MYB转录因子在植物耐盐基因工程中的应用进展[J]. 浙江农业学报, 2020, 32(10): 1910-1920.
[5]	蒋丰千, 李旸, 余大为, 孙敏, 张恩宝. 基于Caffe卷积神经网络的大豆病害检测系统[J]. 浙江农业学报, 2019, 31(7): 1177-1183.
[6]	刘慧洁, 徐恒, 邱文怡, 李晓芳, 张华, 朱英, 李春寿, 王良超. 转录因子在植物生长发育及非生物逆境响应的作用[J]. 浙江农业学报, 2019, 31(7): 1205-1214.
[7]	彭成璐, 张瑜, 丁雪东, 李玉, 冯士彬, 王希春, 李锦春, 吴金节. 11S通过NF-κB信号通路引起猪小肠上皮细胞损伤[J]. 浙江农业学报, 2019, 31(3): 384-391.
[8]	彭成璐, 张瑜, 夏晓冬, 贺濛初, 舒迎霜, 冯士彬, 李玉, 王希春, 李锦春, 吴金节. β-伴大豆球蛋白通过p38/JNK MAPK信号通路引起IPEC-J2细胞损伤[J]. 浙江农业学报, 2019, 31(2): 242-249.
[9]	张旭, 王小佳, 黎思辰, 董甜甜, 汪志辉. 柑橘果实粒化过程中木质素生物合成与调控研究进展[J]. 浙江农业学报, 2019, 31(12): 2131-2140.
[10]	刘鹏, 金诚谦, 印祥, 宁新杰, 李庆伦. 大豆联合收获机清选装置与关键技术研究进展[J]. 浙江农业学报, 2019, 31(10): 1758-1766.
[11]	胡志辉, 汪艳杰, 张丽琴. 菜用大豆施肥后荧光、光谱、光合等参数对产量的预测[J]. 浙江农业学报, 2018, 30(8): 1355-1362.
[12]	吴伟, 冯志娟, 徐盛春, 刘娜, 张古文, 胡齐赞, 龚亚明. 大豆NIP类水孔蛋白基因的鉴定及表达特性分析[J]. 浙江农业学报, 2018, 30(7): 1101-1109.
[13]	张瑜, 王元红, 汤雪冰, 彭成璐, 舒迎霜, 曹程鸣, 冯士彬, 李玉, 王希春, 吴金节. 大豆抗原蛋白对仔猪的免疫作用[J]. 浙江农业学报, 2018, 30(4): 560-567.
[14]	顾正敏, 李臻峰, 宋飞虎, 张君生, 庄为. 基于光场相机的大豆冠层叶面积无损测量方法研究[J]. 浙江农业学报, 2018, 30(12): 2144-2152.
[15]	邹琪琪, 齐姗姗, 谢录翰, 辛琪, 李俊乐, 张梦宁, 葛欣. 细菌单杂交方法在筛选甲基营养菌甲醇脱氢酶启动子结合蛋白中的应用[J]. 浙江农业学报, 2018, 30(10): 1705-1714.