马铃薯茄镰孢菌干腐病生防菌株的筛选、鉴定及其防效

doi:10.3969/j.issn.1004-1524.2019.07.10

浙江农业学报 ›› 2019, Vol. 31 ›› Issue (7): 1105-1111.DOI: 10.3969/j.issn.1004-1524.2019.07.10

马铃薯茄镰孢菌干腐病生防菌株的筛选、鉴定及其防效

刘治会¹, 郝蓉蓉¹, 许永锋², 杨成德^1,*, 张俊莲³

1.甘肃农业大学植物保护学院/甘肃省农作物病虫害生物防治工程实验室,甘肃兰州 730070;
2.甘肃省张掖市植保植检站,甘肃张掖 734000;
3.甘肃省作物遗传改良与种质创新重点实验室,甘肃兰州730070

收稿日期:2018-12-27 出版日期:2019-07-25 发布日期:2019-08-07
通讯作者: *杨成德,E-mail: yangcd@gsau.edu.cn
作者简介:刘治会(1993—),女,甘肃靖远人,硕士研究生,研究方向为植物保护与植物病理学。E-mail: 1844313326@qq.com
基金资助:
国家马铃薯产业技术体系(CARS-10-P18); 国家自然科学基金(31660148)

Screening, identification and biocontrol effect of microbial antagonist against Fusarium solani causing potato dry rot

LIU Zhihui¹, HAO Rongrong¹, XU Yongfeng², YANG Chengde^1,*, ZHANG Junlian³

1. College of Plant Protection, Gansu Agricultural University/Biocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province, Lanzhou 730070, China;
2. Zhangye Station of Plant Protection and Quarantine, Zhangye 734000,China;
3. Gansu Key Lab of Crop Improvement & Germplasm Enhancement, Lanzhou 730070,China;

Received:2018-12-27 Online:2019-07-25 Published:2019-08-07

摘要/Abstract

摘要： 以茄镰孢菌(Fusarium solani)为指示菌,利用平板对峙法从120株内生细菌中筛选出30株抑菌率高于70.00%的菌株,其中265ZY1、265ZY3和265XY6对茄镰孢菌具有较强的拮抗能力,其抑菌率分别为72.20%、73.00%和72.80%,按1∶1∶1复配的菌肥对马铃薯干腐病防效达79.75%,表明265ZY1、265ZY3和265XY6具有良好的抑菌防病作用,具有开发为马铃薯干腐病生防菌剂的潜力;经形态观察及16S rDNA基因序列分析,将265ZY1鉴定为死谷芽孢杆菌(Bacillus vallismortis),265ZY3鉴定为解淀粉芽孢杆菌(Bacillus amyloliquefaciens),265XY6鉴定为甲基营养型芽孢杆菌(Bacillus methylotrophicus)。

关键词: 茄镰孢菌, 生防菌, 死谷芽孢杆菌, 淀粉芽孢杆菌, 甲基营养型芽孢杆菌, 防效

Abstract: In this study, Fusarium solani was used as indicative fungi, 30 antagonistic bacteria whose inhibition rates were higher than 70.00% from 120 endophytic bacteria were selected with the method of plate confrontation. Among them, 265ZY1, 265ZY3 and 265XY6 had strong antagonistic effects on F. solani, the inhibition rates reached 72.20%, 73.00% and 72.80%, respectively. Combining them with the ratio of 1∶1∶1, the inhibition rate reached 79.75%. The results showed that 265ZY1, 265ZY3 and 265XY6 had excellent biological functions, which could be developed as biocontrol bacteria. With the observation of morphology and 16S rDNA gene sequence analysis, 265ZY1, 265ZY3 and 265XY6 were identified as Bacillus vallismortis, Bacillus amyloliquefaciens and Bacillus methylotrophicus, respectively.

Key words: Fusarium solani, microbial antagonist, Bacillus vallismortis, Bacillus amyloliquefaciens, Bacillus methylotrophicus, control effect

中图分类号:

S435.32

刘治会, 郝蓉蓉, 许永锋, 杨成德, 张俊莲. 马铃薯茄镰孢菌干腐病生防菌株的筛选、鉴定及其防效[J]. 浙江农业学报, 2019, 31(7): 1105-1111.

LIU Zhihui, HAO Rongrong, XU Yongfeng, YANG Chengde, ZHANG Junlian. Screening, identification and biocontrol effect of microbial antagonist against Fusarium solani causing potato dry rot[J]. , 2019, 31(7): 1105-1111.

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马铃薯茄镰孢菌干腐病生防菌株的筛选、鉴定及其防效

Screening, identification and biocontrol effect of microbial antagonist against Fusarium solani causing potato dry rot

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