浙江农业学报 ›› 2019, Vol. 31 ›› Issue (12): 1987-1995.DOI: 10.3969/j.issn.1004-1524.2019.12.06

• 动物科学 • 上一篇    下一篇

新疆褐牛产奶和繁殖性状候选基因功能注释

卢鑫1, 周靖航1, 杨朝云1, 张梦华2, 叶连萌1, 李叔臻1, 黄锡霞2, 马云1, 王兴平1, 史远刚1,*   

  1. 1.宁夏大学 农学院,宁夏 银川 750021;
    2.新疆农业大学 动物科学学院,新疆 乌鲁木齐 830052
  • 收稿日期:2019-06-27 出版日期:2019-12-25 发布日期:2019-12-25
  • 通讯作者: 史远刚,E-mail:shyga818@126.com
  • 作者简介:卢鑫(1996-),女,辽宁朝阳人,硕士研究生,研究方向为新疆褐牛分子数量遗传学。E-mail: 13889236591@163.com
  • 基金资助:
    现代农业(奶牛)产业技术体系(CARS-36)

Functional annotation of candidate genes for milk production and reproductive traits in Xinjiang Brown cattle

LU Xin1, ZHOU Jinghang1, YANG Chaoyun1, ZHANG Menghua2, YE Lianmeng1, LI Shuzhen1, HUANG Xixia2, MA Yun1, WANG Xingping1, SHI Yuangang1,*   

  1. 1. School of Agriculture, Ningxia University, Yinchuan 750021, China;
    2. College of Animal Science and Technology, Xinjiang Agricultural University, Urumqi 830052, China
  • Received:2019-06-27 Online:2019-12-25 Published:2019-12-25

摘要: 基于新疆褐牛产奶和繁殖性状全基因组关联分析研究结果,对候选基因进行生物信息学功能分析,进一步筛选出与新疆褐牛产奶和繁殖性状显著相关的关键基因。根据关联分析结果中显著单核苷酸多肽位点的物理位置共找到55个候选基因,其中49个基因可在数据库中检索到相应功能注释信息。GO分类结果显示,这些基因涉及代谢、转录等30个GO条目;KEGG代谢通路富集分析结果显示,候选基因富集于7个通路中,其中基因数最多的是Wnt信号通路和钙黏素信号通路。通过查阅各候选基因相关研究进展和相关基因的生物学功能、生理生化分析,发现对产奶性状候选基因中的CDH2、GABRG2和繁殖性状候选基因中的EPRS基因可进行下一步的基因功能研究。

关键词: 新疆褐牛, 全基因组关联分析, 功能注释, 产奶性状, 繁殖性状

Abstract: Based on the results of whole genome association analysis of milk production and reproductive traits of Xinjiang brown cattle, the bioinformatics function of candidate genes were analyzed, and the key genes significantly related to milk production and reproductive traits of Xinjiang Brown cattle were screened out. According to the results of association analysis, 55 candidate genes were found on the basis of physical location of significant single nucleotide polypeptide sites, of which 49 genes could retrieve the corresponding functional annotation information in the database. The results of GO classification showed that these genes were involved in metabolism, transcription and other 30 GO terms. The enrichment analysis of KEGG metabolic pathway showed that the candidate genes were enriched in 7 pathways, of which the largest number of genes were Wnt signaling pathway and cadherin signaling pathway. By reviewing the research progress of the candidate genes and the biological function, physiological and biochemical analysis of the related genes, the next step of gene function study can be carried out on CDH2 and GABRG2 gene in the candidate genes for milk production traits and EPRS gene in the candidate genes for reproductive traits.

Key words: Xinjiang Brown cattle, genome-wide association analysis, function annotation, milk traits, reproduction traits

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