金盏菊根尖细胞染色体制片与核型分析

doi:10.3969/j.issn.1004-1524.2020.01.11

浙江农业学报 ›› 2020, Vol. 32 ›› Issue (1): 86-92.DOI: 10.3969/j.issn.1004-1524.2020.01.11

金盏菊根尖细胞染色体制片与核型分析

龙海梅^1,2, 张楠卿¹, 李宗艳^1,2,*, 王琴¹, 付超¹

1. 西南林业大学国家林业和草原局西南风景园林工程中心,云南昆明 650224;
2. 西南林业大学功能花卉研究中心,云南昆明 650224

收稿日期:2019-05-27 出版日期:2020-01-25 发布日期:2020-03-11
通讯作者: ^*李宗艳,E-mail: lizyan74@sina.com
作者简介:龙海梅(1995—),女,云南文山人,助理农艺师,主要从事园林植物资源研究。E-mail: 2280343619@qq.com
基金资助:
教育部大学生创新创业实践项目(201710677005); 云南省大学生创新创业实践项目(201710677005)

Study on chromosome technique of Calendula officinalis and its karyotype

LONG Haimei^1,2, ZHANG Nanqing¹, LI Zongyan^1,2,*, WANG Qin¹, FU Chao¹

1. Southwest Engineering Research Center of Landscape Architecture, National Forestry and Grassland Administration, Southwest Forestry University, Kunming 650224, China;
2. Functional Flower Center, Southwest Forestry University, Kunming 650224, China

Received:2019-05-27 Online:2020-01-25 Published:2020-03-11

摘要/Abstract

摘要： 以金盏菊根尖为材料,分析取材时间、根长、预处理时间与试剂种类对染色体制片效果的影响,并研究其核型。结果表明:在根长为1.0~1.5 cm、上午8:30至9:30取材,能获得较多有丝分裂中期相的细胞;用对二氯苯饱和溶液在6 ℃处理金盏菊6 h,可以获得浓缩程度适宜且形态清晰的染色体。通过染色体制片可以观察到,金盏菊常染色体数为28,包括中部着丝点和近中部着丝点染色体;染色体相对长度为5.93%~8.46%,相对长度系数为0.83~1.18,臂比最大值为2.34;金盏菊体细胞中有多达11~18条B染色体,B染色体有棒状和点状形式,其核型公式为2n=18m+10sm,为2B类型。

关键词: 金盏菊, 根尖, 染色体制片, 核型分析

Abstract: In order to explore the optimal chromosome technique of Calendula officinalis, its root tips were used to investigate the effects of sampling time, root length, pretreatment methods and different reagent. The results indicated that Calendula officinalis could maintain vigorous mitotic-activity with more cells at mitotic metaphase if the roots were sampled from 8:30 am to 9:30 am when they had 1.0-1.5 cm length. It was better for Calendula officinalis to be treated with saturated solution of p-dichlorobenzene for 6 h at 6 ℃. It showed that the autosomes number of Calendula officinalis was 28 consisting of median region and submedian region types of chromosomes. Moreover, the percentage of all chromosome' relative length varied from 5.93% to 8.46%. The mean indice of relative length ranged from 0.83 to 1.18. Maximum value of arm ratio was 2.34. Besides standard chromosomes, there were bar-like or point-like B-chromosomes in its somatic cells and its number varied from 11 to 18. The analysis showed that the karyotype formula was 2n=18m+10sm and its karyotype belonged to 2B based on Stebbins classification system.

Key words: Calendula officinalis, root tip, chromosome technique, karyotype analysis

中图分类号:

S667.3

龙海梅, 张楠卿, 李宗艳, 王琴, 付超. 金盏菊根尖细胞染色体制片与核型分析[J]. 浙江农业学报, 2020, 32(1): 86-92.

LONG Haimei, ZHANG Nanqing, LI Zongyan, WANG Qin, FU Chao. Study on chromosome technique of Calendula officinalis and its karyotype[J]. , 2020, 32(1): 86-92.

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金盏菊根尖细胞染色体制片与核型分析

Study on chromosome technique of Calendula officinalis and its karyotype

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