三株产吲哚乙酸根际促生芽孢杆菌的筛选鉴定及其促生作用

doi:10.3969/j.issn.1004-1524.2021.05.13

浙江农业学报 ›› 2021, Vol. 33 ›› Issue (5): 873-884.DOI: 10.3969/j.issn.1004-1524.2021.05.13

三株产吲哚乙酸根际促生芽孢杆菌的筛选鉴定及其促生作用

李福艳(), 刘晓玉, 颜静婷, 蔡燕飞^*()

华南农业大学资源环境学院,广东广州 510642

收稿日期:2020-10-25 出版日期:2021-05-25 发布日期:2021-05-25
通讯作者: 蔡燕飞
作者简介:*蔡燕飞,E-mail:yanfeicai@scau.edu.cn
李福艳(1993—),女,山东济南人,硕士研究生,主要从事土壤微生物研究。E-mail:lifuyan0916@163.com
基金资助:
广东省重点领域研发计划(2019B020218009);国家自然科学基金委员会(NSFC)-广东省人民政府联合基金(U1701236)

Isolation and identification of three indole-3-acetic acid producing plant-growth-promoting rhizosphere Bacillus sp. and their growth-promoting effects

LI Fuyan(), LIU Xiaoyu, YAN jingting, CAI Yanfei^*()

College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China

Received:2020-10-25 Online:2021-05-25 Published:2021-05-25
Contact: CAI Yanfei

摘要/Abstract

摘要：

为丰富具有产吲哚乙酸(IAA)功能的芽孢杆菌菌种资源,开发高效根际促生微生物菌肥,从玉米、生菜和绿化带植被的根际土壤中筛选产IAA的芽孢杆菌,最终获得3株高效产IAA的菌株——YC9L、YC3172和YC5064,其IAA产量分别为46.72、79.75、67.55 mg·L^-1。对其进行理化特性和16S rDNA序列分析,鉴定YC9L和YC3172菌株为大猩猩芽孢杆菌(Bacillus massiliogorillae),YC5064菌株为栗褐芽孢杆菌(Bacillus badius)。玉米种子发芽试验显示,YC9L和YC5064菌株对玉米种子发芽,以及幼苗生长具有促进作用。生菜盆栽试验显示,YC9L、YC3172、YC5064菌株对生菜植株生长具有促进效果。玉米盆栽试验显示,YC9L、YC3172、YC5064菌株对玉米植株生长有良好的促进效果,且以YC9L的效果最优。YC9L、YC3172、YC5064菌株均具有解钾能力;YC9L和YC3172菌株具有解磷能力;YC9L、YC3172、YC5064菌株对镉、锌、铜均具有耐性。

关键词: 吲哚乙酸, 芽孢杆菌, 根际促生菌

Abstract:

In order to enrich the resources of strains with the function of indole-3-acetic acid (IAA) production, hence to develop high-efficiency plant-growth-promoting microbial fertilizer, three strains with high IAA production ability were isolated from the rhizosphere soil of maize, lettuce and green belt vegetation plant, and were encoded as YC9L, YC3172 and YC5064, respectively. The IAA production of YC9L, YC3172 and YC5064 reached 46.72, 79.75, 67.55 mg·L^-1, respectively. According to the characteristics and 16S rDNA identification, YC9L and YC3172 strains were identified as Bacillus massiliogorillae, and YC5064 strain was identified as Bacillus badius. The maize seed germination test showed that YC9L and YC5064 strains could promote seed germination and seedling growth of maize. The lettuce pot experiment showed that YC9L, YC3172, YC5064 strains had obvious promoting effect on the growth of lettuce. The maize pot experiment showed that YC9L, YC3172, YC5064 strains could promote the growth of maize. In addition, YC9L, YC3172 and YC5064 strains all had the ability to dissolve potassium. YC9L and YC3172 strains had the ability to dissolve phosphorus. YC9L, YC3172 and YC5064 strains had resistance to Cd, Zn and Cu.

Key words: indole-3-acetic acid, Bacillus, plant-growth-promoting rhizobacteria

中图分类号:

S154.39

李福艳, 刘晓玉, 颜静婷, 蔡燕飞. 三株产吲哚乙酸根际促生芽孢杆菌的筛选鉴定及其促生作用[J]. 浙江农业学报, 2021, 33(5): 873-884.

LI Fuyan, LIU Xiaoyu, YAN jingting, CAI Yanfei. Isolation and identification of three indole-3-acetic acid producing plant-growth-promoting rhizosphere Bacillus sp. and their growth-promoting effects[J]. Acta Agriculturae Zhejiangensis, 2021, 33(5): 873-884.

图/表 14

图1 所筛菌株的产IAA能力定性结果

Fig.1 Qualitative results of IAA produced by strains

图2 所筛菌株的产IAA能力定量结果柱上无相同字母的表示差异显著(P<0.05)。下同。

Fig.2 Quantitative results of IAA produced by strains Bars marked without the same letters indicated significant difference at P<0.05. The same as below.

图3 菌株的革兰氏染色与芽孢染色图

Fig.3 Microscopy results of Gram staining and spore staining of strains

图4 菌株溶血试验结果

Fig.4 Hemolysis results of strains

图5 基于YC9L、YC3172、YC5064菌株16S rDNA基因序列的系统发育树

Fig.5 Phylogenetic tree of YC9L, YC3172, YC5064 strains based on 16S rDNA gene sequences

表1 不同处理对玉米种子发芽的影响

Table 1 Effect of different treatments on maize seed germination

处理Treatment	株高Plant height/cm	根长Root length/cm
CK	6.17±0.32 b	2.70±0.31 c
YC9L	7.74±0.48 a	8.18±0.52 a
YC5064	7.18±0.38 ab	4.86±0.33 b

图6 YC9L、YC5064菌株对玉米种子发芽的促进作用

Fig.6 Growth promoting effect of YC9L, YC5064 strains on maize seed germination

表2 不同处理对生菜植株生物量的影响

Table 2 Effect of different treatments on lettuce biomass indexes

处理 Treatment	株高 Plant height/cm	根长 Root length/cm	叶长 Leaf length/cm	茎粗 Stem thickness/mm	叶宽 Leaf width/cm	鲜重 Fresh weight/g
CK	9.19±0.38 b	10.81±0.55 b	8.41±0.36 b	2.87±0.05 c	5.34±0.24 b	3.25±0.27 b
YC9L	10.82±0.48 a	10.74±0.58 b	10.27±0.46 a	3.66±0.16 b	6.32±0.27 a	4.91±0.42 a
YC3172	10.69±0.39 a	13.06±0.56 a	10.23±0.37 a	3.55±0.09 ab	6.52±0.18 a	5.19±0.31 a
YC5064	11.06±0.31 a	11.17±0.59 b	9.91±0.47 a	3.90±0.09 a	6.33±0.24 a	5.09±0.34 a

图7 YC9L、YC3172和YC5064菌株对生菜的促生效果

Fig.7 Growth promoting effect of YC9L, YC3172, YC5064 strains on lettuce growth

表3 不同处理对玉米植株生物量的影响

Table 3 Effect of different treatments on biomass indexes of maize

处理 Treatment	株高 Plant height/cm	叶长 Leaf length/cm	根长 Root length/cm	茎粗 Stem thickness/mm	叶宽 Leaf width/cm	根干重 Root dry weight/g	叶干重 Leaf dry weight/g
CK	39.07±0.60 b	29.64±0.45 b	24.97±0.92 a	4.81±0.09 b	2.15±0.04 b	0.24±0.02 b	0.37±0.01 b
YC9L	41.55±0.61 a	31.85±0.45 a	27.30±0.90 a	5.73±0.15 a	2.37±0.06 a	0.34±0.03 a	0.46±0.02 a
YC3172	38.79±0.68 b	29.99±0.50 b	27.75±0.87 a	5.58±0.14 a	2.31±0.07 ab	0.29±0.02 ab	0.39±0.02 b
YC5064	39.97±0.46 ab	31.01±0.47 ab	26.67±1.68 a	5.40±0.11 a	2.33±0.06 a	0.28±0.01 b	0.41±0.01 ab

图8 YC9L、YC3172、YC5064菌株对玉米的促生效果

Fig.8 Growth promoting effect of YC9L, YC3172, YC5064 strains on maize growth

图9 YC9L、YC3172、YC5064菌株的解钾能力

Fig.9 Potassium-dissolving characteristics of strain YC9L, YC3172, YC5064

图10 YC9L、YC3172、YC5064菌株的解磷能力

Fig.10 Phosphorus-dissolving characteristics of strain YC9L, YC3172, YC5064

图11 不同菌株对重金属镉、锌、铜胁迫的敏感性

Fig.11 Sensitivity of different stains to Cd, Zn, Cu stress

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三株产吲哚乙酸根际促生芽孢杆菌的筛选鉴定及其促生作用

Isolation and identification of three indole-3-acetic acid producing plant-growth-promoting rhizosphere Bacillus sp. and their growth-promoting effects

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