不同种质番茄材料抗番茄黄化曲叶病毒病特性研究

doi:10.3969/j.issn.1004-1524.2021.11.11

浙江农业学报 ›› 2021, Vol. 33 ›› Issue (11): 2085-2097.DOI: 10.3969/j.issn.1004-1524.2021.11.11

不同种质番茄材料抗番茄黄化曲叶病毒病特性研究

王贤(), 刘放, 魏小红^*(), 朱晓林, 王宝强

甘肃农业大学生命科学技术学院,甘肃省作物遗传改良与种质创新重点实验室,甘肃省干旱生境作物学重点实验室,甘肃兰州 730070

收稿日期:2020-08-22 出版日期:2021-11-25 发布日期:2021-11-26
通讯作者: 魏小红
作者简介:*魏小红,E-mail: weixh@gsau.edu.cn
王贤(1997—),男,甘肃天水人,硕士研究生,研究方向为植物学。E-mail: 1431268954@qq.com
基金资助:
国家自然科学基金(31560663)

Study on resistance of different tomato germplasm materials to yellow leaf curl virus disease

WANG Xian(), LIU Fang, WEI Xiaohong^*(), ZHU Xiaolin, WANG Baoqiang

College of Life Science and Technology, Gansu Agricultural University, Gansu Key Lab of Crop Genetic & Germplasm Enhancement, Gansu Provincial Key Lab of Aridland Crop Science, Lanzhou 730070, China

Received:2020-08-22 Online:2021-11-25 Published:2021-11-26
Contact: WEI Xiaohong

摘要/Abstract

摘要：

以不同种质番茄为实验材料,利用苗期农杆菌接种法接种番茄黄化曲叶病毒(tomato yellow leaf curl virus, TYLCV),采用实时定量PCR和生理生化方法评价对TYLCV的抗性,统计分析不同抗性番茄材料不同发病时期的表型特征、带毒率、抗氧化酶活性、总酚与类黄酮含量及抗性基因表达量的变化情况,并分析各抗性指标之间的关系。结果表明,番茄材料带毒率为100%;853没有任何感病症状,802与803感病最为严重;抗氧化酶活性、总酚与类黄酮含量及抗性基因表达量在各时期均较高的有867、857、853,817与842次之,803最低。SOD活性、CAT活性、APX活性、总酚含量、类黄酮含量、Ty-1基因、Ty-2和Ty-3基因的表达量相互之间均呈极显著正相关(总酚与Ty-1之间为显著正相关,P<0.01)。番茄植株感染TYLCV后,Ty-1的表达相比于其他抗病基因具有延迟性。综合分析筛选出867、857、853三种抗病性较强的材料,植株体内的抗性基因、抗氧化酶、总酚与类黄酮之间呈正相关,共同提高了番茄植株对TYLCV的抗性。

关键词: 番茄, 番茄黄化曲叶病毒病, 抗氧化酶, 次生代谢物, 抗性基因, 抗性

Abstract:

Different tomato materials were inoculated with tomato yellow leaf curl virus (TYLCV) by Agrobacterium tumefaciens infiltration. Real-time quantitative PCR and physiological and biochemical methods were used to evaluate the resistance to TYLCV. The phenotypic characteristics, virus carrying rate, antioxidant enzyme activity, secondary metabolite content and resistance gene expression of tomato materials with different resistance were analyzed. The results showed that the virus infection rates of tomato materials were 100%; the tested variety 853 had no symptoms, while 802 and 803 were the most susceptible; the varieties 867, 857 and 853 had higher antioxidant enzyme activity, contents of total phenols and flavonoids, and higher resistance gene expression, followed by 817 and 842, the lowest was 803. SOD activity, CAT activity, APX activity, total phenol content, flavonoid content, Ty-1 gene, Ty-2 and Ty-3 gene expression levels were significantly positively correlated with each other (total phenol and Ty-1 showed significantly positive correlation, P<0.01). Compared with other disease resistance genes, the expression of Ty-1 was delayed after tomato plants were infected with TYLCV. The resistance genes, antioxidant enzymes, total phenols and flavonoids in plants were significantly positively correlated, which jointly improved the resistance of tomato plants to TYLCV.

Key words: tomato, tomato yellow leaf curl virus disease, antioxidant enzymes, secondary metabolites, resistance gene, resistance

中图分类号:

S641.2

王贤, 刘放, 魏小红, 朱晓林, 王宝强. 不同种质番茄材料抗番茄黄化曲叶病毒病特性研究[J]. 浙江农业学报, 2021, 33(11): 2085-2097.

WANG Xian, LIU Fang, WEI Xiaohong, ZHU Xiaolin, WANG Baoqiang. Study on resistance of different tomato germplasm materials to yellow leaf curl virus disease[J]. Acta Agriculturae Zhejiangensis, 2021, 33(11): 2085-2097.

图/表 13

表1 不同番茄材料的基因型

Table 1 Genotypes of different tomato materials

编号 Number	基因型 Genotype	编号 Number	基因型 Genotype
801	ty-1/ty-1+ty-2/ty-2+ty-3/ty-3	822	ty-1/ty-1+ty-3/ty-3
802	ty-1/ty-1+ty-2/ty-2+ty-3/ty-3	841	ty-1/ty-1+ty-2/ty-2+ty-3/ty-3
803	ty-1/ty-1+ty-2/ty-2+ty-3/ty-3	842	ty-1/ty-1+ty-2/ty-2+ty-3/ty-3
805	ty-1/ty-1+ty-2/ty-2+ty-3/ty-3	853	Ty-1/Ty-1+ty-2/ty-2+Ty-3/Ty-3
806	ty-1/ty-1+ty-2/ty-2+ty-3/ty-3	857	Ty-1/Ty-1+ty-2/ty-2+Ty-3/Ty-3
817	ty-1/ty-1+ty-2/ty-2+ty-3/ty-3	867	Ty-1/Ty-1+ty-2/ty-2+Ty-3/Ty-3
818	Ty-1/Ty-1+ty-2/ty-2+ty-3/ty-3	齐达利Qidali	Ty-1/Ty-1+ty-2/ty-2+Ty-3a/ty-3a
819	ty-1/ty-1+ty-2/ty-2+ty-3/ty-3	MM	无
820	Ty-1/Ty-1+ty-2/ty-2+ty-3/ty-3

表2 抗病基因及引物序列

Table 2 Disease resistance genes and primer sequences

基因 Gene	引物序列 Primer sequence(5'-3')
Ty-1	F:GGCAAAATATGCAGCCAGGCTTTCC R:TCAGTATGTATACGAGGTTCGCCGT
Ty-2	F:TGCGGCTGTTTCCTATGGTT R:TGGTTCTGGAAAGGGATCGAA
Ty-3	F:TGAGCGGAGAAATGTGACAG R:CGGGGCTTTGATGAAACGAA
actin	F:GAAATAGCATAAGATGGCAGACG R:ATACCCACCATCACACCAGTAT

图1 部分品种感病后的表型 A,对照;B,感病后表型。

Fig.1 The phenotypes of some varieties after virus infection A, The control; B, The phenotype after infection.

图2 不同番茄材料接种20 d后的PCR扩增结果

Fig.2 PCR amplification results of different tomato materials after virus inoculation for 20 days

图3 不同番茄材料接种病毒后超氧化物歧化酶(SOD)活性的变化情况柱上没有相同小写字母表示差异显著(P<0.05)。下同。

Fig.3 Changes of superoxide dismutase (SOD) activity in different tomato materials after virus inoculation The bars without the same lowercase letters showed significant difference(P<0.05). The same as below.

图4 不同番茄材料接种病毒后过氧化氢酶(CAT)活性的变化情况

Fig.4 Changes of catalase (CAT) activity in different tomato materials after virus inoculation

图5 不同番茄材料接种病毒后抗坏血酸过氧化物酶(APX)活性的变化情况

Fig.5 Changes of ascorbate peroxidase (APX) activity in different tomato materials after virus inoculation

图6 不同番茄材料接种病毒后总酚含量的变化情况

Fig.6 Changes of total phenol content in different tomato materials after virus inoculation

图7 不同番茄材料接种病毒后类黄酮含量的变化情况

Fig.7 Changes in the content of flavonoids in different tomato materials after virus inoculation

图8 不同番茄材料接种病毒后Ty-1基因的表达情况

Fig.8 Expression of Ty-1 gene in different tomato materials after virus inoculation

图9 不同番茄材料接种病毒后Ty-2基因的表达情况

Fig.9 Expression of Ty-2 gene in different tomato materials after virus inoculation

图10 不同番茄材料接种病毒后Ty-3基因的表达情况

Fig.10 Expression of Ty-3 gene in different tomato materials after virus inoculation

表3 各抗性指标的相关性分析

Table 3 Correlation analysis of resistance indexes

	SOD	CAT	APX	总酚 Total phenols	类黄酮 Flavonoids	Ty-1	Ty-2	Ty-3
SOD	1
CAT	0.757^**	1
APX	0.867^**	0.690^**	1
总酚Total phenols	0.802^**	0.699^**	0.739^**	1
类黄酮Flavonoids	0.702^**	0.797^**	0.682^**	0.798^**	1
Ty-1	0.635^**	0.658^**	0.568^**	0.356^*	0.527^**	1
Ty-2	0.734^**	0.753^**	0.702^**	0.631^**	0.705^**	0.698^**	1
Ty-3	0.754^**	0.762^**	0.703^**	0.631^**	0.786^**	0.732^**	0.822^**	1

参考文献 36

[1]	柯红娇. 番茄黄化曲叶病毒病的生物防治[D]. 南京:南京农业大学, 2014.
	KE H J. The study of biological control against tomato yellow leaf curl virus[D]. Nanjing: Nanjing Agricultural University, 2014. (in Chinese with English abstract)
[2]	杨悦俭, 周国治, 王荣青, 等. 抗番茄黄化曲叶病毒病品种种植中的问题与对策[J]. 中国蔬菜, 2011(21):1-4.
	YANG Y J, ZHOU G Z, WANG R Q, et al. Problems and countermeasures in planting tomato varieties with resistance to tomato yellow leaf curl virus[J]. China Vegetables, 2011(21):1-4.(in Chinese)
[3]	丁铭, 岳宁, 董家红, 等. 侵染番茄的中国番茄黄化曲叶病毒致病性相关分子的遗传多样性[J]. 云南大学学报(自然科学版), 2008, 30(S1):63-68.
	DING M, YUE N, DONG J H, et al. Genetic diversity of tomato yellow leaf curl China virus associated satellites DNA β infecting Solanum lycopersicon[J]. Journal of Yunnan University (Natural Sciences Edition), 2008, 30(S1):63-68.(in Chinese with English abstract)
[4]	倪光荣, 胡会英, 陈永顺, 等. 番茄黄化曲叶病毒病的发生规律与防控措施[J]. 西北园艺(蔬菜), 2010(5):34-35.
	NIE G R, HU H Y, CHEN Y S, et al. Occurrence regularity and control measures of Tomato yellow leaf curl virus[J]. Northwest Horticulture, 2010(5):34-35. (in Chinese)
[5]	孙胜, 亢秀萍, 邢国明, 等. 番茄黄化曲叶病毒病研究进展[J]. 东北农业大学学报, 2015, 46(5):102-108.
	SUN S, KANG X P, XING G M, et al. Research progress on the tomato yellow leaf curl virus disease[J]. Journal of Northeast Agricultural University, 2015, 46(5):102-108.(in Chinese with English abstract)
[6]	骆巧娟. 樱桃番茄黄化曲叶病毒病的鉴定及果实营养特性的研究[D]. 兰州:甘肃农业大学, 2019.
	LUO Q J. Identification of cherry tomato yellow leaf curl virus disease and study on fruit nutritional characteristics[D]. Lanzhou: Gansu Agricultural University, 2019. (in Chinese with English abastract)
[7]	邹芳斌, 司龙亭, 李新, 等. 黄瓜枯萎病抗性与防御系统几种酶活性关系的研究[J]. 华北农学报, 2008, 23(3):181-184.
	ZOU F B, SI L T, LI X, et al. The relation between the resistance of the cucumber wilt and the activities of several enzyme in defense system[J]. Acta Agriculturae Boreali-Sinica, 2008, 23(3):181-184.(in Chinese with English abstract)
[8]	HERNÁNDEZ I, CELA J, ALEGRE L, et al. Antioxidant defenses against drought stress[M]// Plant responses to drought stress. Berlin, Heidelberg: Springer Berlin Heidelberg, 2012: 231-258.
[9]	LUO X Y. Supplementing conventional treatment with pycnog enol (R) may improve hepatitis C virus associated type 2 diabetes: a mini review[J]. Journal of Clinical and Translational Hepatology, 2016 (3):228-233.
[10]	张树生, 胡蕾, 刘忠良, 等. 植物体内抗病相关酶与植物抗病性的关系[J]. 安徽农学通报, 2006, 12(13):48-49.
	ZHANG S S, HU L, LIU Z L, et al. Relationship between the disease defense-related enzymes and the disease resistance of plants[J]. Anhui Agricultural Science Bulletin, 2006, 12(13):48-49.(in Chinese with English abstract)
[11]	于景金, 范宁丽, 李冉, 等. 高浓度CO₂对热胁迫条件下高羊茅生长和抗氧化系统的影响[J]. 草业学报, 2017, 26(8):113-122.
	YU J J, FAN N L, LI R, et al. Effects of elevated carbon dioxide concentration on the growth and antioxidant system in tall fescue under heat stress[J]. Acta Prataculturae Sinica, 2017, 26(8):113-122.(in Chinese with English abstract)
[12]	JI J Y, YANG J, ZHANG B W, et al. Sodium pheophorbide a controls cherry tomato gray mold (Botrytis cinerea) by destroying fungal cell structure and enhancing disease resistance-related enzyme activities in fruit[J]. Pesticide Biochemistry and Physiology, 2020, 166:104581. DOI URL
[13]	FREEMAN B C, BEATTIE G A. An overview of plant defenses against pathogens and herbivores[J]. Plant Health Instructor, 2008, 149:489-490.
[14]	邱永祥, 柯玉琴, 代红军, 等. 甘薯抗蔓割病的酚类物质代谢的研究[J]. 中国生态农业学报, 2007, 15(5):167-170.
	QIU Y X, KE Y Q, DAI H J, et al. Phenolics metabolism in sweet potato infected by Fusarium oxysporum Schlecht.f.sp[J]. Chinese Journal of Eco-Agriculture, 2007, 15(5):167-170.(in Chinese with English abstract)
[15]	薛美昭, 仪慧兰. 果实抗病防御应答参与SO₂对‘红提’葡萄的采后保鲜[J]. 应用与环境生物学报, 2017, 23(5):806-810.
	XUE M Z, YI H L. Induction of disease defense responses by SO₂ during the preservation of ‘Red Globe’ grapes[J]. Chinese Journal of Applied and Environmental Biology, 2017, 23(5):806-810.(in Chinese with English abstract)
[16]	杨欢欢, 许向阳, 姜景彬, 等. 番茄抗黄化曲叶病基因ty-5的分子标记及遗传分析[J]. 园艺学报, 2015, 42(10):1965-1973.
	YANG H H, XU X Y, JIANG J B, et al. Genetic analysis and gene mapping of tomato yellow leaf curl virus resistant gene ty-5[J]. Acta Horticulturae Sinica, 2015, 42(10):1965-1973.(in Chinese with English abstract)
[17]	VIDAVSKI F, CZOSNEK H, GAZIT S, et al. Pyramiding of genes conferring resistance to tomato yellow leaf curl virus from different wild tomato species[J]. Plant Breeding, 2008, 127(6):625-631. DOI URL
[18]	张少丽, 邵景成, 胡志峰. 番茄黄化曲叶病毒(TY)侵染性克隆接种鉴定方法研究[J]. 甘肃农业科技, 2014(10):16-19.
	ZHANG S L, SHAO J C, HU Z F. Study on inoculated identification techniques for infectious clones to tomato yellow leaf curl virus (TY)[J]. Gansu Agricultural Science and Technology, 2014(10):16-19.(in Chinese with English abstract)
[19]	李佳蔚. 番茄Ty基因对TYLCV复制、转录和移动的影响及TYLCV侵染后番茄的转录组分析[D]. 北京:中国农业科学院, 2016.
	LI J W. The effect of different Ty-genes on TYLCV duplication, transcription and moving, and transcription of tomato plant response to TYLCV[D]. Beijing: Chinese Academy of Agricultural Sciences, 2016. (in Chinese with English abstract)
[20]	HU L X, HUANG Z H, LIU S Q, et al. Growth response and gene expression in antioxidant-related enzymes in two bermudagrass genotypes differing in salt tolerance[J]. Journal of the American Society for Horticultural Science, 2012, 137(3):134-143. DOI URL
[21]	裴斌, 张光灿, 张淑勇, 等. 土壤干旱胁迫对沙棘叶片光合作用和抗氧化酶活性的影响[J]. 生态学报, 2013, 33(5):1386-1396.
	PEI B, ZHANG G C, ZHANG S Y, et al. Effects of soil drought stress on photosynthetic characteristics and antioxidant enzyme activities in Hippophae rhamnoides Linn.seedings[J]. Acta Ecologica Sinica, 2013, 33(5):1386-1396.(in Chinese with English abstract)
[22]	唐巧玉, 周毅峰, 朱玉昌, 等. 金橘皮中黄酮类物质的提取及其体外抗氧化活性研究[J]. 农业工程学报, 2008, 24(6):258-261.
	TANG Q Y, ZHOU Y F, ZHU Y C, et al. Extraction of flavone compounds from Fortunella margarita peel and its antioxidation in vitro[J]. Transactions of the Chinese Society of Agricultural Engineering, 2008, 24(6):258-261.(in Chinese with English abstract)
[23]	PASTRANA-BONILLA E, AKOH C C, SELLAPPAN S, et al. Phenolic content and antioxidant capacity of muscadine grapes[J]. Journal of Agricultural and Food Chemistry, 2003, 51(18):5497-5503. DOI URL
[24]	LATTANZIOV, LATTANZIOVM T, CARDINALIA. Role of phenolics in the resistance mechanisms of plants against fungal pathogens and insects[A]. ResearchSignpost: Kerala, 2006: 23-67.
[25]	HATFIELD R, VERMERRIS W. Lignin formation in plants. the dilemma of linkage specificity[J]. Plant Physiology, 2001, 126(4):1351-1357. DOI URL
[26]	庄炳昌, 王玉民, 谢雪菊, 等. 抗性不同大豆品种感染灰斑病后若干生化反应[J]. 作物学报, 1993, 19(6):567-569.
	ZHUANG B C, WANG Y M, XIE X J, et al. Changes of some biochemical characters of soybean with different resistant levels infected by Cercospora sojina Hara[J]. Acta Agronomica Sinica, 1993, 19(6):567-569.(in Chinese)
[27]	林玲, 卢江, 黄羽, 等. 不同葡萄品种感染霜霉病菌后叶片中几种酶活性的变化[J]. 南方农业学报, 2014, 45(2):222-225.
	LIN L, LU J, HUANG Y, et al. Enzyme activities of different grape varieties infected by Plasmopara viticola[J]. Journal of Southern Agriculture, 2014, 45(2):222-225.(in Chinese with English abstract)
[28]	王玲平. 黄瓜感染枯萎病菌后生理生化变化及其与抗病性关系的研究[D]. 太谷:山西农业大学, 2001.
	WANG L P. Study on the Physiological and Biochemical Changes of Cucumber Infected with Fusarium Wilt and Its Relationship with Disease Resistance[D]. Taigu: Shanxi Agricultural University, 2001. (in Chinese with English abstract)
[29]	丁九敏, 高洪斌, 刘玉石, 等. 黄瓜霜霉病抗性与叶片中生理生化物质含量关系的研究[J]. 辽宁农业科学, 2005(1):11-13.
	DING J M, GAO H B, LIU Y S, et al. Study on the relationship between cucumber downy mildew resistance and the content of physiological and biochemical substances in leaves[J]. Liaoning Agricultural Sciences, 2005(1):11-13. (in Chinese)
[30]	贾双双, 徐坤. 南方根结线虫侵染对2个不同抗性番茄砧木幼苗苯丙烷类代谢的影响[J]. 热带亚热带植物学报, 2016, 24(2):143-150.
	JIA S S, XU K. Effects of infection with Meloidogyne incognita on phenylpropanes metabolism in two different resistant tomato rootstock seedlings[J]. Journal of Tropical and Subtropical Botany, 2016, 24(2):143-150.(in Chinese with English abstract)
[31]	赵秀娟, 唐鑫, 程蛟文, 等. 酶活性、丙二醛含量变化与苦瓜抗枯萎病的关系[J]. 华南农业大学学报, 2013, 34(3):372-377.
	ZHAO X J, TANG X, CHENG J W, et al. The relationship between malonaldehyde content, enzymatic changes and the resistance of bitter gourd to Fusarium wilt[J]. Journal of South China Agricultural University, 2013, 34(3):372-377.(in Chinese with English abstract)
[32]	齐绍武, 官春云, 刘春林. 甘蓝型油菜品系一些酶的活性与抗菌核病的关系[J]. 作物学报, 2004, 30(3):270-273.
	QI S W, GUAN C Y, LIU C L. Relationship between some enzyme activity and resistance to Sclerotinia sclerotiorum of rapeseed cultivars[J]. Acta Agronomica Sinica, 2004, 30(3):270-273.(in Chinese with English abstract)
[33]	李艳, 姜俊, 赵红星, 等. 番茄抗黄化曲叶病毒病研究进展[J]. 农业科技通讯, 2017(6):10-13.
	LI Y, JIANG J, ZHAO H X, et al. Research progress of tomato resistance to yellow leaf curl virus[J]. Bulletin of Agricultural Science and Technology, 2017(6):10-13. (in Chinese)
[34]	胡恩美, 余文贵, 王银磊, 等. 番茄抗黄化曲叶病基因研究进展[J]. 江苏农业学报, 2013, 29(6):1496-1502.
	HU E M, YU W G, WANG Y L, et al. Research progress in tomato yellow leaf curl virus resistance genes[J]. Jiangsu Journal of Agricultural Sciences, 2013, 29(6):1496-1502.(in Chinese with English abstract)
[35]	刘榛, 张延安. CAPS标记在番茄抗黄化曲叶病毒病基因Ty1和Ty3遗传关系分析上的应用[J]. 西北农业学报, 2013, 22(4):92-96.
	LIU Z, ZHANG Y A. CAPS marker use in genetic relationship analysis between Ty1 and Ty3 resistant gene to tomato yellow leaf curl virus[J]. Acta Agriculturae Boreali-Occidentalis Sinica, 2013, 22(4):92-96.(in Chinese with English abstract)
[36]	田兆丰, 刘伟成, 厚凌宇, 等. 不同番茄品种对番茄黄化曲叶病毒的抗病性分析[J]. 中国农业科学, 2016, 49(14):2850-2856.
	TIAN Z F, LIU W C, HOU L Y, et al. Analysis of resistance of different tomato varieties to tomato yellow leaf curl virus in tomato[J]. Scientia Agricultura Sinica, 2016, 49(14):2850-2856.(in Chinese with English abstract)

不同种质番茄材料抗番茄黄化曲叶病毒病特性研究

Study on resistance of different tomato germplasm materials to yellow leaf curl virus disease

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