›› 2010, Vol. 22 ›› Issue (1): 0-104.

• 论文 •    

杏鲍菇担孢子交配型的鉴定分析

冯伟林1,2,蔡为明2,*,金群力2 ,范丽军2 ,刘晓红3   

  1. 1浙江大学 农业与生物技术学院,浙江 杭州 310029;2浙江省农业科学院 园艺研究所,浙江 杭州 310021;3安徽农业大学 生命科学学院,安徽 合肥 230001
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-01-25 发布日期:2010-01-25

Analysis of mating type of Basidispores in Pleurotus eryngii

FENG Wei-lin;CAI Wei-ming;*;JIN Qun-li;FAN Li-jun;LIU Xiao-hong   

  1. 1 College of Agricultural and Biological Technology,Zhejiang University, Hangzhou 310029, China;2 Institute of Horticulture,Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;College of Life Sciences, Anhui Agricultural University, Hefei 230036, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-01-25 Published:2010-01-25

摘要: 利用单孢稀释法,显微镜下观察分离得到72个杏鲍菇有性孢子单核体,通过单核两两交配,观察到3种典型的菌落形态,初步得到4种不同的交配型单核菌株;应用ISSR分子标记技术,对初步确定的四种不同交配型单核菌丝进行DNA指纹分析,筛选得到7条引物进行扩增,结果显示共计扩增出56条清晰易辨的多态性DNA条带,其中4个菌株共有条带9条,多态性条带47条,结果表明所得到的单核菌株确定为4种不同的交配型;ISSR分子标记技术可以作为食用菌单核菌株鉴别及指纹分析的有效工具。

关键词: 杏鲍菇, 交配型, 菌落形态, ISSR

Abstract: Using the method of single spore dilution, 72 monocytes were isolated from the microscopic observation. Through the mating between every two groups of single core strains, 3 kinds of typical colony morphylogy were observed and four different mating types of monocyte strains were obtained. Analysis of DNA fingerprinting for those four types of strains was investigated by ISSR molecular marker technology, and 7 pairs of primers were screened out for amplification. The results showed that 56 DNA polymorphism bands were amplified clearly and easily, among which 9 bands were all observed in the four types of strains and 47 polymorphic bands were observed. It was indicated that the single-core strain was identified as four different strains of the mating type. ISSR molecular markers can be used as an effective tool for identification of single-core strain and fingerprint analysis of edible fungus strains.

Key words: Pleurotus eryngii, mating type, colony morphology, ISSR