胡柚青果干中柚皮苷和新橙皮苷的同步提取、纯化及其功能活性分析

doi:10.3969/j.issn.1004-1524.20250529

浙江农业学报 ›› 2026, Vol. 38 ›› Issue (6): 1153-1163.DOI: 10.3969/j.issn.1004-1524.20250529

胡柚青果干中柚皮苷和新橙皮苷的同步提取、纯化及其功能活性分析

韦丽萍¹(), 王阳光¹^,^*(), 杨英¹, 陆胜民²^,^*()

¹ 浙江海洋大学食品与药学学院, 浙江舟山 316022
² 浙江省农业科学院食品科学研究所, 农产品质量安全全国重点实验室,全省生鲜食品智慧物流与加工重点实验室, 浙江杭州 310021

收稿日期:2025-08-11 出版日期:2026-06-25 发布日期:2026-07-14
作者简介:韦丽萍,研究方向为食品加工与安全。E-mail:2293613854@qq.com
通讯作者: ^*王阳光,E-mail:634449563@qq.com;陆胜民,E-mail:1010508971@qq.com
基金资助:
浙江省“尖兵领雁+X”科技计划(2025C01101);浙江省重点研发计划(2017C02004)

Synchronous extraction, purification, and functional activity analysis of naringin and neohesperidin from dried immature Huyou (Citrus ×aurantium Changshanhuyou) fruits

WEI Liping¹(), WANG Yangguang¹^,^*(), YANG Ying¹, LU Shengmin²^,^*()

¹ Food and Pharmacy College, Zhejiang Ocean University, Zhoushan 316022, Zhejiang, China
² State Key Laboratory for Quality and Safety of Agro-Products, Zhejiang Key Laboratory of Intelligent Logistics and Processing of Fresh Products, Institute of Food Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2025-08-11 Published:2026-06-25 Online:2026-07-14

摘要/Abstract

摘要：

为高效提取胡柚青果干(衢枳壳原料)中富含的柚皮苷和新橙皮苷,本研究采用超声辅助乙醇浸提法进行单因素同步提取实验,并通过响应面法进行优化;选用AB-8大孔吸附树脂对粗提物进行纯化,并对纯化前后的提取物进行体外抗氧化和降糖活性比较。结果表明,在乙醇体积分数43%、超声时间14 min、超声功率300 W、料液比1∶16(g·mL^-1)条件下,柚皮苷和新橙皮苷的提取得率分别为133.19 mg·g^-1和99.23 mg·g^-1,总得率为232.42 mg·g^-1;纯化后提取物中柚皮苷和新橙皮苷的纯度分别提高了2.47和1.74百分点,且DPPH自由基清除率、ABTS自由基清除率和α-葡萄糖苷酶抑制率均显著(p<0.05)提高。以上研究表明,该提取和纯化工艺简单、有效,且所得提取物具有较好的功能活性,为胡柚青果中活性成分的高效提取和利用提供了技术基础。

关键词: 胡柚青果, 柚皮苷, 新橙皮苷, 超声辅助提取, 响应面分析, 大孔树脂纯化, 功能活性

Abstract:

In order to efficiently extract naringin and neohesperidin enriched in dried immature Huyou (Citrus × aurantium Changshanhuyou, original medicinal material of Qu Aurantii Fructus) fruits, a synchronous extraction process using ultrasound-assisted ethanol extraction was carried through single-factor experiments, and the extraction conditions were optimized using response surface methodology. AB-8 macroporous resin was selected to purify the crude extract, and the in vitro antioxidative and hypoglycemic activities of the extracts before and after purification were compared. The results showed that under the conditions of ethanol volume fraction 43%, ultrasonic time 14 min, ultrasonic power 300 W, and solid-liquid ratio 1∶16 (g·mL^-1), the yields of naringin and neohesperidin reached 133.19 mg·g^-1and 99.23 mg·g^-1, respectively, with a total yield of 232.42 mg·g^-1. After purification, the purity of naringin and neohesperidin increased by 2.47 and 1.74 percentage points, respectively, and their DPPH and ABTS free radical scavenging rates, as well as α-glucosidase inhibition rate, were all significantly (p<0.05) improved. The results indicated that the extraction and purification process was simple and effective, and the extract exhibited good functional activities, providing a technological basis for the efficient extraction and application of bioactive compounds from dried immature Huyou fruits.

Key words: immature Huyou fruit, naringin, neohesperidin, ultrasound-assisted extraction, response surface analysis, macroporous resin purification, functional activity

中图分类号:

韦丽萍, 王阳光, 杨英, 陆胜民. 胡柚青果干中柚皮苷和新橙皮苷的同步提取、纯化及其功能活性分析[J]. 浙江农业学报, 2026, 38(6): 1153-1163.

WEI Liping, WANG Yangguang, YANG Ying, LU Shengmin. Synchronous extraction, purification, and functional activity analysis of naringin and neohesperidin from dried immature Huyou (Citrus ×aurantium Changshanhuyou) fruits[J]. Acta Agriculturae Zhejiangensis, 2026, 38(6): 1153-1163.

图/表 9

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水平 Level	A/%	B/min	C/W	D/(g·mL^-1)
-1	20	5	250	1∶5
0	40	15	300	1∶15
1	60	25	350	1∶25

水平 Level	A/%	B/min	C/W	D/(g·mL^-1)
-1	20	5	250	1∶5
0	40	15	300	1∶15
1	60	25	350	1∶25

A/%	B/min	C/W	D/(g·mL^-1)	NR得率/(mg·g^-1) NR yield/(mg·g^-1)	NHP得率/(mg·g^-1) NHP yield/(mg·g^-1)	总得率/(mg·g^-1) Total yield/(mg·g^-1)
40	15	250	1∶25	94.08	74.14	168.22
20	25	300	1∶15	82.70	68.65	151.35
40	15	350	1∶25	104.07	91.99	196.06
40	5	300	1∶5	83.66	72.33	155.99
60	25	300	1∶15	94.49	80.31	174.80
20	5	300	1∶15	85.43	73.90	159.33
20	15	300	1∶5	89.71	74.59	164.30
40	15	300	1∶15	132.79	98.10	230.89
40	25	250	1∶15	81.11	66.26	147.37
40	25	300	1∶25	91.24	72.87	164.11
40	5	300	1∶25	98.34	78.82	177.16
40	25	350	1∶15	81.96	73.43	155.39
60	15	250	1∶15	96.46	80.33	176.79
60	15	350	1∶15	110.83	77.21	188.04
40	5	350	1∶15	95.42	77.59	173.01
60	15	300	1∶5	93.46	68.66	162.12
40	15	350	1∶5	90.61	79.11	169.72
40	5	250	1∶15	83.83	68.49	152.32
20	15	350	1∶15	88.97	79.63	168.60
40	15	300	1∶15	135.65	104.23	239.88
40	15	300	1∶15	130.03	104.18	234.21
60	15	300	1∶25	98.87	88.90	187.77
20	15	250	1∶15	84.53	74.54	159.07
40	15	300	1∶15	128.65	101.60	230.25
40	25	300	1∶5	83.93	74.68	158.61
20	15	300	1∶25	88.38	71.13	159.52
40	15	250	1∶5	89.83	80.01	169.84
60	5	300	1∶15	100.86	82.47	183.33
40	15	300	1∶15	130.89	105.68	236.57

A/%	B/min	C/W	D/(g·mL^-1)	NR得率/(mg·g^-1) NR yield/(mg·g^-1)	NHP得率/(mg·g^-1) NHP yield/(mg·g^-1)	总得率/(mg·g^-1) Total yield/(mg·g^-1)
40	15	250	1∶25	94.08	74.14	168.22
20	25	300	1∶15	82.70	68.65	151.35
40	15	350	1∶25	104.07	91.99	196.06
40	5	300	1∶5	83.66	72.33	155.99
60	25	300	1∶15	94.49	80.31	174.80
20	5	300	1∶15	85.43	73.90	159.33
20	15	300	1∶5	89.71	74.59	164.30
40	15	300	1∶15	132.79	98.10	230.89
40	25	250	1∶15	81.11	66.26	147.37
40	25	300	1∶25	91.24	72.87	164.11
40	5	300	1∶25	98.34	78.82	177.16
40	25	350	1∶15	81.96	73.43	155.39
60	15	250	1∶15	96.46	80.33	176.79
60	15	350	1∶15	110.83	77.21	188.04
40	5	350	1∶15	95.42	77.59	173.01
60	15	300	1∶5	93.46	68.66	162.12
40	15	350	1∶5	90.61	79.11	169.72
40	5	250	1∶15	83.83	68.49	152.32
20	15	350	1∶15	88.97	79.63	168.60
40	15	300	1∶15	135.65	104.23	239.88
40	15	300	1∶15	130.03	104.18	234.21
60	15	300	1∶25	98.87	88.90	187.77
20	15	250	1∶15	84.53	74.54	159.07
40	15	300	1∶15	128.65	101.60	230.25
40	25	300	1∶5	83.93	74.68	158.61
20	15	300	1∶25	88.38	71.13	159.52
40	15	250	1∶5	89.83	80.01	169.84
60	5	300	1∶15	100.86	82.47	183.33
40	15	300	1∶15	130.89	105.68	236.57

方差来源 Source of variance	平方和 Sum of squares	自由度 Degrees of freedom	均方 Mean square	F值 F value	p
模型Model	21 700.21	14	1 550.02	45.43	<0.000 1
A	1 020.84	1	1 020.84	29.92	<0.000 1
B	204.27	1	204.27	5.99	0.028 2
C	496.78	1	496.78	14.56	0.001 9
D	435.13	1	435.13	12.75	0.003 1
AB	0.08	1	0.08	2.22×10^-3	0.963 1
AC	0.74	1	0.74	0.02	0.885 0
AD	231.50	1	231.50	6.78	0.020 8
BC	40.13	1	40.13	1.18	0.296 4
BD	61.39	1	61.39	1.80	0.201 2
CD	195.44	1	195.44	5.73	0.031 3
A²	6 002.44	1	6 002.44	175.92	<0.000 1
B²	10 745.46	1	10 745.46	314.94	<0.000 1
C²	6 536.21	1	6 536.21	191.57	<0.000 1
D²	6 081.63	1	6 081.63	178.25	<0.000 1
残差Residual	477.67	14	34.12	—	—
失拟项Lack of fit	413.36	10	41.34	2.57	0.188 0
纯误差Pure error	64.31	4	16.08	—	—
总回归Total regression	22 177.88	28	—	—	—

胡柚青果干中柚皮苷和新橙皮苷的同步提取、纯化及其功能活性分析

Synchronous extraction, purification, and functional activity analysis of naringin and neohesperidin from dried immature Huyou (Citrus ×aurantium Changshanhuyou) fruits

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参考文献 28

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树脂种类 Resin type	NR吸附率 NR adsorption rate	NR解吸率 NR desorption rate	NHP吸附率 NHP adsorption rate	NHP解吸率 NHP desorption rate
AB-8	91.26±0.18 a	62.12±2.89 a	92.86±0.15 a	62.01±2.91 a
D101	86.03±0.36 c	62.46±2.28 a	88.03±0.32 c	63.55±3.01 a
HPD-100	89.25±0.12 b	63.36±3.09 a	91.03±0.09 b	62.56±3.78 a
HPD-300	82.77±0.72 d	62.32±3.70 a	84.97±0.58 d	62.65±2.63 a

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