20个烟草品种低温逆境下发芽和苗期生理生化特性的变化及耐寒性评价

›› 2011, Vol. 23 ›› Issue (2): 0-238.

• 作物科学 •

20个烟草品种低温逆境下发芽和苗期生理生化特性的变化及耐寒性评价

马文广1,崔华威2,李永平1,郑昀晔1,韩瑞2,胡晋2,*

1 云南烟草科学研究院,云南玉溪 653100；2 浙江大学农业与生物技术学院种子科学中心,浙江杭州 310029

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2011-03-25 发布日期:2011-03-25

Effects of low temperature stress on physiological and biochemical characteristics of 20 tobacco cultivars and assessment of their cold tolerance at germination and seedling stages

MA Wen-guang;CUI Hua-wei;LI Yong-ping;ZHENG Yun-ye;HAN Rui;HU Jin;*

1 Yunan Tobacco Scientific Research Institute, Yuxi 653100, China;2 Seed Science Center of Agriculture and Biotechnology College, Zhejiang University, Hangzhou 310029, China

Received:1900-01-01 Revised:1900-01-01 Online:2011-03-25 Published:2011-03-25

摘要/Abstract

摘要： 试验对低温逆境下20个烟草品种发芽和苗期生理生化特性变化进行分析,并对其耐寒性进行鉴定。在常温(20～30℃)和低温(11℃)逆境下测定种子发芽特性及幼苗生长、生理生化等指标,将各性状在低温与常温下测定值的比值作为耐寒性评价指标。相关性分析表明,相对发芽率,相对发芽指数,相对活力指数和相对丙二醛(MDA)含量之间显著相关,是较好的耐寒性评价指标。通过聚类分析,将20个品种分为耐寒型、中等耐寒型和低温敏感型3类,聚类结果与相关性分析结果一致。

关键词: 低温逆境, 烟草, 苗期, 耐寒性, 聚类分析

Abstract: The cold tolerance of 20 tobacco cultivars at germination and seedling stages were investigated by determining germination characteristics, seedling physiological and biochemical characteristics at normal temperature (20-30℃) and low temperature (11℃) stress. The ratios (relative values) of traits at low temperature to those at normal temperature were used to assess cold tolerance. The correlation analysis showed that the relative values of germination percentage, germination index, vigor index and malonaldehyde content were significantly correlated to each other, which were suitable for evaluating cold tolerance. By cluster analysis, 20 tobacco cultivars were grouped into three classes: cold tolerance, intermediate cold tolerance and cold sensitivity, and the results were consistent with that of correlation analysis.

Key words: low temperature stress, tobacco, seedling stage, cold tolerance, cluster analysis

马文广;崔华威;李永平;郑昀晔;韩瑞;胡晋;*. 20个烟草品种低温逆境下发芽和苗期生理生化特性的变化及耐寒性评价[J]. , 2011, 23(2): 0-238.

MA Wen-guang;CUI Hua-wei;LI Yong-ping;ZHENG Yun-ye;HAN Rui;HU Jin;*. Effects of low temperature stress on physiological and biochemical characteristics of 20 tobacco cultivars and assessment of their cold tolerance at germination and seedling stages[J]. , 2011, 23(2): 0-238.

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20个烟草品种低温逆境下发芽和苗期生理生化特性的变化及耐寒性评价

Effects of low temperature stress on physiological and biochemical characteristics of 20 tobacco cultivars and assessment of their cold tolerance at germination and seedling stages

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