Cloning and encoding protein bioinformatics analysis of BdCDPK4 from Brachypodium distachyon

doi:10.3969/j.issn.1004-1524.2017.02.23

›› 2017, Vol. 29 ›› Issue (2): 345-352.DOI: 10.3969/j.issn.1004-1524.2017.02.23

• Biosystems Engineering • Previous Articles

Cloning and encoding protein bioinformatics analysis of BdCDPK4 from Brachypodium distachyon

WEI Shuya¹, ZHAO Xudong², WANG Huiping¹, YANG Guangxiao², HE Guangyuan²

1. Institute of Applied Bio-technology, College of Biological Engineering, Wuhan Polytechnic, Wuhan 430074, China;
2. The Genetic Engineering International Cooperation Base of Ministry of Science and Technology, Key Laboratory of Molecular Biophysics of Chinese Ministry of Education, College of Life Science and Technology, Huazhong University of Science & Technology, Wuhan 430074, China;

Received:2016-10-01 Online:2017-02-15 Published:2017-03-06

Abstract

Abstract: Brachypodium distachyon, the genome of which shares high homology with wheat and barley, has been used as the new model species of Gramineae. In order to clarify the structure and function of Brachypodium distachyon CDPKs gene, the coding region sequence (CDS) of BdCDPK4 gene of Brachypodium distachyon was isolated, and its character, structure and function were analyzed by bioinformatics methods. The results showed that 1 851 bp cDNA (GenBank ID: XM_003559516) of coding sequence of BdCDPK4 gene included the complete 1 752 bp CDS, coded 583 amino acids, and located on chloroplast and vacuole. The molecular weight and isoelectric point of BdCDPK4 protein were 64.78 ku and 9.12, respectively. BdCDPK4 protein contained several domains including N-variable domain, kinase domain, autoinhibitory domain, EF-hands and CaM-like domain. Phylogenetic analysis results suggested that BdCDPK4 protein had high homology with wheat TaCDPK19. TaCDPK19 were induced by high salt and white powder Blumeria graministritici(Bgt). BdCDPK4 protein may participate in the regulation of signal transduction pathways under stresses according to the homologies of protein domain among all species. Taken together, our data could establish a good foundation for studying the biological functions of BdCDPK4 gene.

Key words: CDPKs, BdCDPK4, multiple sequence alignment, bioinformatics analysis

CLC Number:

Q949.71⁺4.2

WEI Shuya, ZHAO Xudong, WANG Huiping, YANG Guangxiao, HE Guangyuan. Cloning and encoding protein bioinformatics analysis of BdCDPK4 from Brachypodium distachyon[J]. , 2017, 29(2): 345-352.

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Cloning and encoding protein bioinformatics analysis of BdCDPK4 from Brachypodium distachyon

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