Resistance mechanism of bio-control strain DZSY21 against maize sheath blight

doi:10.3969/j.issn.1004-1524.2017.03.15

›› 2017, Vol. 29 ›› Issue (3): 450-459.DOI: 10.3969/j.issn.1004-1524.2017.03.15

• Plant Protection • Previous Articles Next Articles

Resistance mechanism of bio-control strain DZSY21 against maize sheath blight

SU Bo¹, JIANG Hubiao², QIAO Jianli³, PAN Jie², XU Wei², WU Anning², DING Ting^{2, *}

1. Anhui Provincial Key Laboratory of Crop Biology, Hefei 230036, China;
2.School of Plant Protection, Anhui Agricultural University, Hefei 230036, China;
3.Institute of Plant Protection and Agricultural Chemistry, Nanyang Academy of Agricultural Sciences, Nanyang 473083, China

Received:2016-09-21 Online:2017-03-20 Published:2017-03-31

Abstract

Abstract: This study was carried out to explicit the resistance mechanism of endophytic bacteria DZSY21 against maize sheath blight. The colonization property of biocontrol strain DZSY21 in maize leaf sheath was analysed by antibiotic label method, and resistance of maize treated with DZSY21 at different growth stages to sheath blight were assessed through field experiments, then the related enzymes activity and expression of PR-1 and PR-2a gene in maize leaf sheath treated with DZSY21 at different growth stages were detected by spectrophotometry and quantitative real-time PCR. The results indicated that DZSY21 could successfully colonize maize leaf sheath, it had a symbiotic relationship with maize leaf sheath, and colonization of DZSY21 in maize leaf sheath could significantly enhance maize resistance to maize sheath blight. Activities of polyphenol oxidase (PPO), peroxidase (POD), phenylalnine ammonialyase (PAL) and expression of defense response genes (PR-1, PR-2a) in maize leaf sheath treated with DZSY21 were significantly higher than those of normal control groups at different growth stages. The results afforded scientific basis for the further research on bio-controlling mechanism and DZSY21 application.

Key words: maize sheath blight, polyphenol oxidase, peroxidase, phenylalnine ammonialyase, defense response gene, colonization

CLC Number:

S435.13

SU Bo, JIANG Hubiao, QIAO Jianli, PAN Jie, XU Wei, WU Anning, DING Ting. Resistance mechanism of bio-control strain DZSY21 against maize sheath blight[J]. , 2017, 29(3): 450-459.

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Resistance mechanism of bio-control strain DZSY21 against maize sheath blight

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