Studies on immunomodulatory and antioxidant activities in vitro of recombinant human lactoferrin from transgenic rice

doi:10.3969/j.issn.1004-1524.2018.01.01

›› 2018, Vol. 30 ›› Issue (1): 1-6.DOI: 10.3969/j.issn.1004-1524.2018.01.01

• Crop Science • Previous Articles Next Articles

Studies on immunomodulatory and antioxidant activities in vitro of recombinant human lactoferrin from transgenic rice

QIN Yi¹, LYU Guoying², ZHANG Zuofa^{2, *}

1. Technology Transfer Center, Zhejiang University, Hangzhou 310058, China;
2. Institute of Horticulture, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2017-06-29 Online:2018-01-20 Published:2018-02-09

Abstract

Abstract: In the present study, the immunomodulatory and antioxidant activities of the recombinant human lactoferrin (rhLF) were studied. The antioxidant activities of rhLF were investigated by DPPH, ABTS and hydroxyl radical scavenging activities and the immunomodulatory activities were determined by the detection of cell proliferation and phagocytic activity of RAW264.7. It turns out that rhLF exhibited scavenging activity against DPPH, ABTS and OH· radical as well as immunomodulatory activities. rhLF possessed excellent antioxidant and immunomodulatory activities, and was the important resource of the exploitation of health-care food.

Key words: recombinant human lactoferrin, antioxidant activity, immunomodulatory activity

CLC Number:

S511

QIN Yi, LYU Guoying, ZHANG Zuofa. Studies on immunomodulatory and antioxidant activities in vitro of recombinant human lactoferrin from transgenic rice[J]. , 2018, 30(1): 1-6.

References

[1] MASSON P L, HEREMANS J F.Lactoferrin in milk from different species[J]. Comparative Biochemistry and Physiology Part B: Comparative Biochemistry, 1971, 39(1): 119-129.
[2] CHRISTIE G T, ALONSO Z V, ALONSO P, et al.Lactoferrin for prevention of neonatal sepsis[J]. Biometals, 2014, 27(5): 1007-1016.
[3] HUANG N, BEHHELL D, CARD C, et al.Bioactive recombinant human lactoferrin, derived from rice, stimulates mammalian cell growth[J]. In Vitro Cellular & Developmental Biology-Animal, 2008, 44(10): 464-471.
[4] FREIBURGHAUS C, LINFMSTK-MSNDDONi H, PSULDDON M, et al.Reduction of ultraviolet light-induced DNA damage in human colon cancer cells treated with a lactoferrin-derived peptide[J]. Journal of Dairy Science, 2011, 95(10): 5552-5560.
[5] NANDI S, SUZUKI Y A, HUANG J, et al.Expression of human lactoferrin in transgenic rice grains for the application in infant formula[J]. Plant Science, 2002, 163(4): 713-722.
[6] WARD P P, PAZ E, CONNEELY O M.Lactoferrin: Multifunctional roles of lactoferrin: a critical overview[J]. Cellular and Molecular Life Sciences, 2005, 62: 2540-2548.
[7] SALMON V, LEGRAND D, SLOMIANNY M C, et al.Production of human lactoferrin in transgenic tobacco plants[J]. Protein Expression & Purification, 1998, 13(1): 127-135.
[8] RACHMAWATI D, MORI T, HOSAKA T, et al.Production and characterization of recombinant human lactoferrin in transgenic Javanica rice[J]. Breeding Science, 2005, 55(2): 213-222.
[9] CHONG DK, LANGRIDGE WH.Expression of full length bioactive antimicrobial human lactoferrin in potato plants[J]. Transgenic Research, 2000, 9: 71-78.
[10] 赵伊英,刘峰,郑回勇,等. 转人乳铁蛋白基因番茄的表达及其生物活性[J]. 农业生物技术学报,2006, 14(2):299-300.
ZHAO Y Y, LIU F, ZHENG H Y, et al.Expression and bioactivity of human-lactoferrin gene in transgenic tomato[J]. Journal of Agricultural Biotechnology, 2006,14(2): 299-300. (in Chinese)
[11] ANZAI H, TAKAIWA F, KATSUMATA K.Production of human lactoferrin in transgenic plants[C]// International Conference on Lactoferrin: Structure, Canada, 1999.
[12] LIN J Z, ZHOU B, YANG Y Z, et al.Piercing and vacuum infiltration of the mature embryo: a simplified method for Agrobacterium-mediated transformation of indica rice[J]. Plant Cell Reports, 2009, 28: 1065-1074.
[13] 胡贻椿. 转高赖氨酸基因、转人乳铁蛋白基因水稻的食用安全性评价与转豇豆胰蛋白酶抑制剂水稻的外源蛋白表达纯化[D].北京:中国疾病预防控制中心营养与食品安全所, 2010.
HU E C.Safety accessment of genetically modified rice with lysine-rich gene and genetically modified rice with human lactoferrin gene and expression and purification of exogenous protein in genetically modified rice with cowpea trypsin inhibitor gene[D]. Beijing: National Institute of Nutrition and Health Chinese Center for Disease Control and Prevention, 2010.
[14] BETHELL D R, HUANG J M.Recombinant human lactoferrin treatment for global health issues: Iron deficiency and acute diarrhea[J]. Biomethals, 2004,17(3):337-342.
[15] TWYMAN R M, STOGER E, SCHILLBERG S, et al.Molecular farming in plants: Host systems and expression technology[J]. Trends in Biotechnology, 2003, 21(12): 570-578.
[16] BHAN M K, ARORA N K, KHOSHOO V, et al.Comparion of a lactose-free cereal-based fromular and cow’s milk in infants and children with acute gastroenteritis[J]. Journal of Pediatric Gastroenterology & Nutrition, 1988, 7(2):208-213.
[17] GASTANADUY A, CORDANO A, GRAHAM G G.Accept ability, tolerance and nutritional value of a rice-based infant formula[J]. Journal of Pediatric Gastroenterology & Nutrition, 1990, 11(2): 240-246.
[18] MESSEGUER J, FOGHER C, GUIDERDONI E, et al.Field assessment of gene flow from transgenic to cultivated rice (Oryza sativa L.) using a herbicide resistance gene as tracer marker[J]. Theoretical & Applied Genetics, 2001, 103(8):1151-1159.
[19] 吴景欢,杨丽琛,刘改革,等. 转基因水稻中重组人乳铁蛋白的分离纯化及其抑菌活性研究[J]. 卫生研究,2013,42(3):399-404.
WU J H, YANG L C, LIU G G, et al.Isolation and purification of recombinant human lactoferrin (rhLF) from transgenic rice and its antibacterial activities[J]. Journal of Hygiene Research, 2013, 42(3):399-404. (in Chinese with English abstract)
[20] ZHANG Z F, LV G Y, PAN H J, et al.Antioxidant and hepatoprotective potential of endo-polysaccharides from Hericium erinaceus grown on tofu whey[J]. International Journal of Biological Macromoleculars, 2012, 51(5): 1140-1146.
[21] MILLER C H, MAHER S G, YOUNG H A.Clinical use of interferon-gamma[J]. Annals of the New York Academy of Sciences, 2009,1182(1): 69-79.
[22] 李婷,王彩云,闫序东,等.乳铁蛋白体外抗氧化性的研究[J].食品科学,2012, 33(21): 111-113.
LI T, WANG C Y, YAN X D, et al.Antioxidant activity of lactoferrin in vitro[J]. Food Science, 2012, 33(21):111-113. (in Chinese with English abstract)
[23] 戴亚妮,于添,朱松,等.重组人乳铁蛋白抗氧化活性研究[J].中国食品卫生杂志,2017,29(3): 251-256.
DAI Y N,YU T, ZHU S, et al.Study on the antioxidant activity of recombinant human lactoferrin[J].Chinese Journal of Food Hygiene, 2017, 29(3): 251-256. (in Chinese with English abstract)
[24] 安清聪,李岑曦,张春勇,等.乳铁蛋白对滇撒配套系断奶仔猪生产性能、血清抗氧化指标及组织抗氧化基因表达的影响[J].动物营养学报,2015,27(7): 2018-2026.
AN Q C, LI C X, ZHANG C Y, et al.Effects of lactoferrin on growth performance, serum antioxidant indices and tissue antioxidant gene expressions of Diansa weaned piglets[J]. Chinese Journal of Animal Nutrition, 2015,27(7): 2018-2026. (in Chinese with English abstract)
[25] 胡志和,李娜,刘传国, 等.乳铁蛋白免疫增强作用评价[J].食品科学,2010,31(11): 244-247.
HU Z H, LI N, LIU C G, et al.Evaluation on immune-enhancement effect of lactoferrin[J].Food Science, 2010,31(11): 244-247. (in Chinese with English abstract)
[26] NAIDI S, SUZUKI A, HUANG J, et al.Expression of human lactoferrin in transgenic rice grains for the application in infant formula[J]. Plant Science, 2002, 163(4):713-722.
[27] HUMPHREY B D, HUANG N, KLASING K C.Rice expressing lactoferrin and lysozyme has antibiotic-like properties when fed to chicks[J]. Journal of Nutrition, 2002,132(6):1214-1218.
[28] SCHYVER A B.Identification of transferrin-and lactoferrin-binding proteins in Haemophilus influenzae[J]. Journal of Medical Microbiology, 1989, 29(2):121-130.

Studies on immunomodulatory and antioxidant activities in vitro of recombinant human lactoferrin from transgenic rice

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