Rapid determination of quinolones in aquatic products by immune colloidal gold method

doi:10.3969/j.issn.1004-1524.2018.02.16

›› 2018, Vol. 30 ›› Issue (2): 290-297.DOI: 10.3969/j.issn.1004-1524.2018.02.16

• Food Science • Previous Articles Next Articles

Rapid determination of quinolones in aquatic products by immune colloidal gold method

LIU Aichun, LIU Chao, ZHANG Le, TONG Chaoming, LI Feng, ZHAO Yun

Hangzhou Academy of Agricultural Sciences, Hangzhou 310024, China

Received:2017-06-20 Online:2018-02-20 Published:2018-02-11

Abstract

Abstract: Antibodies were produced by using the hybridoma cell line QH12, and the highly sensitive immune colloidal gold reagent strip was thus prepared. The lowest detection limits of these stripes for lomefloxacin, ofloxacin, norfloxacin, pefloxacin were 2.0, 2.5, 2.5, 5.0 ng·mL^-1, respectively. The colloidal gold rapid test methods (GICT) for determination of quinolones residues in aquatic products were developed through optimization of sample pretreatment. Detection results of GICT were further compared with that of HPLC method, which was confirmed by LC-MS/MS method. It was shown that the sensitivity of GICT method was higher than that of HPLC. The test results of GICT and LC-MS/MS were in coincidence. The whole detection process of a single sample cost 25 minutes. Comprehensive comparative analysis showed that GICT method was suitable for rapid determination of quinolones in aquatic products.

Key words: quinolones, immune colloidal gold method, aquatic product, rapid test

CLC Number:

LIU Aichun, LIU Chao, ZHANG Le, TONG Chaoming, LI Feng, ZHAO Yun. Rapid determination of quinolones in aquatic products by immune colloidal gold method[J]. , 2018, 30(2): 290-297.

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Rapid determination of quinolones in aquatic products by immune colloidal gold method

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