Expression and distribution of Mel 1b mRNA and protein in various tissues of duck

doi:10.3969/j.issn.1004-1524.2018.05.06

›› 2018, Vol. 30 ›› Issue (5): 711-716.DOI: 10.3969/j.issn.1004-1524.2018.05.06

• Animal Science • Previous Articles Next Articles

Expression and distribution of Mel 1b mRNA and protein in various tissues of duck

LIU Wenju¹, WANG Shujuan^1,*, LIU Xiaoli², PANG Xunsheng¹, WANG Like¹

1. College of Animal Science, Anhui Science and Technology University, Fengyang 233100, China;
2. College of Animal Science, Huazhong Agricultural University, Wuhan 430070, China

Received:2017-09-25 Online:2018-05-20 Published:2018-05-23

Abstract

Abstract: A broad physiological function of melatonin is mediated by binding to melatonin receptors (Mel 1a, Mel 1b and Mel 1c). Melatonin receptors are widely distributed in the body, but vary according to subtypes, species and tissues. The study investigated the expression, distribution of Mel 1b in different tissues of ducks (including heart, spleen, brain, kidney, liver, lung, pancreas and breast muscle) using RT-PCR, real time PCR and immunohistochemistry. The results showed that Mel 1b mRNA was extensively expressed in heart, spleen, brain, kidney, liver, lung, pancreas and breast muscle of ducks, and Mel 1b protein was distributed in brain, lung, liver, skeletal muscle, kidney, heart and pancreas. The expression levels of Mel 1b mRNA were different among various tissues. The highest expression of Mel 1b mRNA was found in ovary, followed by the lung. Compared with the expression level of Mel 1b in brain, the expression levels in spleen, heart kidney and liver were higher, but those in breast muscle and pancreas were lower. Thus, Mel 1b was widely distributed in duck tissues, which further confirmed its involvement in the broad physiological functions of melatonin, especially the regulation of reproduction.

Key words: melatonin receptor, Mel 1b, duck, real-time PCR

CLC Number:

S834

LIU Wenju, WANG Shujuan, LIU Xiaoli, PANG Xunsheng, WANG Like. Expression and distribution of Mel 1b mRNA and protein in various tissues of duck[J]. , 2018, 30(5): 711-716.

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Expression and distribution of Mel 1b mRNA and protein in various tissues of duck

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