Ecological safety assessment of Bcillus cereus D7 isolated from shrimp

doi:10.3969/j.issn.1004-1524.2018.09.20

›› 2018, Vol. 30 ›› Issue (9): 1585-1591.DOI: 10.3969/j.issn.1004-1524.2018.09.20

• Environmental Science • Previous Articles Next Articles

Ecological safety assessment of Bcillus cereus D7 isolated from shrimp

ZHANG Hongyu¹, WANG Haibo^{1, 2}, YANG Yibin³, ZHAO Mingjun⁴, XIA Lei^{1, 2, *}

1. Research & Creation Center of Green Fishery Drug of Chinese Academy of Fishery Sciences, Beijing 100141, China;
2. Beijing Seasun Aquaculture BioTech. Co. Ltd., Beijing 102488, China;
3. Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China;
4. Fishery Development Strategy & Research Centre,Chinese Academy of Fishery Sciences, Beijing 100141;

Received:2018-03-08 Online:2018-09-25 Published:2018-10-15
Contact: 夏磊,E-mail: xialei666@126.com
Supported by:
中国水产科学研究院基本科研业务费专项(2016B002)

Abstract

Abstract: In order to evaluate the ecological safety of Bacillus cereus D7, the effects of Bacillus cereus D7 on concentration of ammonia nitrogen, nitrite and phosphate were tested. The research of safety on Chlorella vulgaris, Daphnia magna, Danio retio, Penaeus vanmamei and Ctenopharyngodon idella were also carried out. The results showed that Bacillus cereus D7 (≥1×10⁷ cfu·mL^-1) reduced ammonia nitrogen and nitrite concentration significantly (P<0.05). When the concentration of Bacillus cereus D7 was below 1×10⁶ cfu·mL^-1, there were no significant influence on ammonia nitrogen and nitrite concentration. When the concentration of Bacillus cereus D7 was between 10⁴~10⁸ cfu·mL^-1, there was no significant influence on phosphate. Concentration of Chlorella vulgaris showed positive correlation with Bacillus cereus D7. The death rate of Daphnia magna, Danio retio,Penaeus Vanmamei had no significant difference after 96 h treatment. When the concentration of Bacillus cereus D7 was between 10⁸~10¹² cfu·mL^-1, there was no obvious influence on death rate of Ctenopharyngodon idella after 96 h. The results showed that Bacillus cereus D7 could have no adverse effects on water and aquatic animals, which could be used as a kind of ecological safety probiotics.

Key words: shrimp source, Bacillus cereus, ecological security

CLC Number:

S966.12

ZHANG Hongyu, WANG Haibo, YANG Yibin, ZHAO Mingjun, XIA Lei. Ecological safety assessment of Bcillus cereus D7 isolated from shrimp[J]. , 2018, 30(9): 1585-1591.

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Ecological safety assessment of Bcillus cereus D7 isolated from shrimp

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