Isolation, identification, and inhibition charateristics of a Fusarium oxysporum f. sp. niveum inhibiting strain

doi:10.3969/j.issn.1004-1524.2018.12.11

›› 2018, Vol. 30 ›› Issue (12): 2065-2071.DOI: 10.3969/j.issn.1004-1524.2018.12.11

• Plant Protection • Previous Articles Next Articles

Isolation, identification, and inhibition charateristics of a Fusarium oxysporum f. sp. niveum inhibiting strain

LYU Zhihang¹, XU Weihui¹, WANG Zhigang^1,*, WANG Chunlong¹, LIU Zeping¹, CHEN Wenjing¹, SHI Yiran^1,2

1. College of Life Science and Agriculture and Forestry, Qiqihar University, Qiqihar 161006, China;
2. The First Hospital of Qigihar, Qiqihar 161005, China

Received:2018-03-30 Online:2018-12-25 Published:2018-12-28

Abstract

Abstract: To isolate a Fusarium oxyxporum f. sp. niveum(FON)-inhibiting strain, the plant rhizosphere soil which was located in Zhalong Nature Reserve, Heilongjiang Province was collected and used. The FON-inhibiting strain was screened out by antagonistic experiment, and was identified by morphological test and 16S rDNA sequence analysis. Moreover, the inhibition characteristics were studied using mycelial growth, spore germination and scanning electron microscope methods. The results showed that the FON-inhibiting strain was classified as Bacillus licheniformis, and was named as MLY01. When the ratio of filtrate to medium was 1∶1, the inhibition rate of FON mycelial growth was 71.85%, the inhibition rate of biomass was 79.55%, and the inhibition rate of spore germination was 76.81% at 36 h. The number of spore was decreased and surface was rough, and the hyphae of FON was deformed after 12 h treatment by cell-free cultural filtrate of MLY01. These results indicated that MLY01 was a high-efficiency FON-inhibiting strain, and it could be used to develop antibacterial agent in future.

Key words: Fusarium oxysporum f.sp.niveum, mycelial growth, spore germination, cell morphology

CLC Number:

LYU Zhihang, XU Weihui, WANG Zhigang, WANG Chunlong, LIU Zeping, CHEN Wenjing, SHI Yiran. Isolation, identification, and inhibition charateristics of a Fusarium oxysporum f. sp. niveum inhibiting strain[J]. , 2018, 30(12): 2065-2071.

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Isolation, identification, and inhibition charateristics of a Fusarium oxysporum f. sp. niveum inhibiting strain

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