Adjuvant effect of E515-A on porcine epidemic diarrhea virus inactivated vaccine

doi:10.3969/j.issn.1004-1524.2019.01.07

›› 2019, Vol. 31 ›› Issue (1): 56-61.DOI: 10.3969/j.issn.1004-1524.2019.01.07

• OAnimal Science • Previous Articles Next Articles

Adjuvant effect of E515-A on porcine epidemic diarrhea virus inactivated vaccine

MA Yongqi, XU Wei, WANG Yuemin, HU Songhua^*

College of Animal Sciences, Zhejiang University, Hangzhou 310058, China

Received:2018-07-03 Online:2019-01-25 Published:2019-04-09

Abstract

Abstract: E515-A was a white oil containing ginseng stem and leaf saponins (GSLS). An oil emulsion vaccine was obtained by mixing E515-A and water phase containing porcine epidemic diarrhea virus (PEDV) antigen at a low shear force (200 r·min^-1). To observe the adjuvant effect of E515-A on inactivated PEDV vaccine, 72 ICR mice were randomly divided into 6 groups and subcutaneously immunized twice at a two-weeks interval with the following solution, respectively: (1) physiological saline solution, (2) PEDV antigen+physiological saline solution, (3) PEDV antigen+GSLS, (4) PEDV antigen+white oil adjuvant, (5) PEDV antigen+E515-A, (6) PEDV antigen+aluminum hydroxide (alum). Serum IFN-γ and NK cytotoxicity were determined 24 h after primary immunization; serum specific IgG, IgG1 and IgG2a and splenic lymphocyte proliferation were analyzed after booster immunization. The results indicated that E515-A significantly enhanced the immune response to PEDV vaccine when compared with aluminum adjuvant. E515-A significantly promoted the killing activity of NK cells in immunized mice, increased the production of IFN-γ, enhanced the splenic lymphocytes proliferation to Concanavalin (Con A) and lipopolysaccharide (LPS) stimulation, and improved the level of serum IgG and its subclass IgG1 and IgG2a in comparison with the control group. To improve the immunity of pigs, E515-A as an adjuvant deserves further study for its use in swine vaccine.

Key words: E515-A, GSLS, PEDV vaccine, adjuvant

CLC Number:

S852.4

MA Yongqi, XU Wei, WANG Yuemin, HU Songhua. Adjuvant effect of E515-A on porcine epidemic diarrhea virus inactivated vaccine[J]. , 2019, 31(1): 56-61.

References

[1] JUNG K, SAIF L J.Porcine epidemic diarrhea virus infection: etiology, epidemiology, pathogenesis and immunoprophylaxis[J]. The Veterinary Journal, 2015, 204(2): 134-143.
[2] 李娇, 王文秀, 谢金文,等. 我国猪流行性腹泻疫苗研究与应用现状[J]. 养猪, 2018 (1):110-112.
LI J, WANG W X, XIE J W, et al.Research and application of porcine epidemic diarrhea vaccine in China[J]. Swine Production, 2018 (1):110-112. (in Chinese)
[3] SONG D, MOON H, KANG B.Porcine epidemic diarrhea: a review of current epidemiology and available vaccines[J]. Clinical and Experimental Vaccine Research, 2015, 4(2): 166-176.
[4] 董世娟, 朱于敏, 于瑞嵩, 等. 我国PEDV分子流行病学研究进展[C]//中国畜牧兽医学会. 中国畜牧兽医学会家畜传染病分会第八次全国会员代表大会暨第15次学术研讨会论文集. 徐州, 2014: 96-101.
[5] 包省军, 宋小明, KEDSIRIN S,等. 人参茎叶皂甙对口蹄疫病毒灭活疫苗免疫后抗体产生的影响[J]. 中国兽医学报, 2009, 29(9):1103-1105.
BAO S J, SONG X M, KEDSIRIN S, et al.Effects of ginsenosides of stems and leaves on immune response to foot and mouth disease attenuated virus vaccine[J]. Chinese Journal of Veterinary Science, 2009, 29(9):1103-1105. (in Chinese with English abstract)
[6] 李禹涛, 燕霞, 谢锋,等. 口蹄疫油乳剂疫苗中添加人参茎叶皂甙对免疫效果和黏滞性的影响[J]. 中国兽医学报, 2013, 33(4):561-565.
LI Y T, YAN X, XIE F, et al.Effects of ginseng stems and leaves saponins (GSLS) on vaccine viscosity and the immune response to foot and mouth disease (FMD) emulsion vaccine[J]. Chinese Journal of Veterinary Science, 2013, 33(4):561-565. (in Chinese with English abstract)
[7] 王明俊, 沈福庆. 矿物油佐剂疫苗研究:Ⅱ、矿物油与乳化剂的选择及乳化方法试验[J]. 中国兽药杂志, 1983 (11):44-48.
WANG M J, SHEN F Q.Study on mineral oil adjuvant vaccine:Ⅱ Selection of mineral oil and emulsifier and test of emulsifying method[J]. Chinese Journal of Veterinary Drug, 1983 (11):44-48. (in Chinese)
[8] LAM V C, LANIER L L.NK cells in host responses to viral infections[J]. Current Opinion in Immunology, 2017, 44:43-51.
[9] LANGEL S N, PAIM F C, LAGER K M, et al.Lactogenic immunity and vaccines for porcine epidemic diarrhea virus (PEDV): historical and current concepts[J]. Virus Research, 2016, 226: 93-107.
[10] SU X, PEI Z, HU S.Ginsenoside Re as an adjuvant to enhance the immune response to the inactivated rabies virus vaccine in mice[J]. International Immunopharmacology, 2014, 20(2): 283-289.
[11] YUAN L, WU L, CHEN J, et al.Paclitaxel acts as an adjuvant to promote both Th1 and Th2 immune responses induced by ovalbumin in mice[J]. Vaccine, 2010, 28(27): 4402-4410.
[12] ZHANG C, WANG Y, WANG M, et al.Rapeseed oil and ginseng saponins work synergistically to enhance Th1 and Th2 immune responses induced by the foot-and-mouth disease vaccine[J]. Clinical and Vaccine Immunology, 2014, 21(8): 1113-1119.
[13] 王萌, 金海波, 张岑容, 等. ZE515 对CP5型金黄色葡萄球菌疫苗的佐剂作用[J]. 中国兽医学报, 2015, 35(8): 1264-1268.
WANG M, JIN H B, ZHANG C R, et al.Efficacy of a Staphylococcus aureus vaccine (CP5 type) adjuvanted with ZE515[J]. Chinese Journal of Veterinary Science, 2015, 35(8): 1264-1268. (in Chinese with English abstract)

Adjuvant effect of E515-A on porcine epidemic diarrhea virus inactivated vaccine

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