›› 2019, Vol. 31 ›› Issue (1): 104-112.DOI: 10.3969/j.issn.1004-1524.2019.01.14

• Plant Protection • Previous Articles     Next Articles

Bioinformatics analysis of odorant-binding proteins and chemosensory proteins of Pieris rapae

ZHU Yu, LIU Yang*   

  1. Wenzhou Vocational College of Science and Technology, Wenzhou 325006, China
  • Received:2018-04-19 Online:2019-01-25 Published:2019-04-09

Abstract: Odorant-binding proteins (OBPs) and chemosensory proteins (CSPs) play an important role in the recognition of odors. The putative OBPs and CSPs protein sequences were found in the cabbage butterfly genome by the local BLAST method. We identified 24 PrOBPs and 38 PrCSPs genes using multiple sequence alignments, cysteine pattern sequence recognition, and evolutionary tree construction. Among them, the 17 OBPs of PrOBP1-PrOBP17 belonged to the Classic OBPs protein family, and the other six OBPs, PrOBP19-PrOBP24, belonged to the Minus-C OBPs protein family. All 38 PrCSPs identified had cysteine pattern recognition sequences of typical lepidoptera CSPs. In this paper, the search and identification of OBPs and CSPs genes in the cabbage butterfly genome enriched the database of insect OBPs and CSPs genes, and also provided references for odor identification and chemical sensing experiments and applications of Pieris rapae.

Key words: Pieris rapae, odorant-binding protein, chemosensory protein

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