Isolation, culture and identification of adipose-derived stem cells from calves

doi:10.3969/j.issn.1004-1524.2019.02.09

›› 2019, Vol. 31 ›› Issue (2): 235-241.DOI: 10.3969/j.issn.1004-1524.2019.02.09

• Animal Science • Previous Articles Next Articles

Isolation, culture and identification of adipose-derived stem cells from calves

WANG Shuaishuai¹, ZHANG Cai^{1, 2, *}, MENG Sudan¹, HUANG Yongzhi¹, ZHU Xuemin¹, WANG Yuqin^{1, 2}, YANG Zijun¹

1. Laboratory of Environment and Livestock Product Safety, Henan University of Science and Technology, Luoyang 471000, China;
2. Henan Sheep Breeding Engineering Research Center, Luoyang 471000, China

Received:2018-06-26 Online:2019-02-25 Published:2019-03-06

Abstract

Abstract: In order to provide seed cells for related research on vegetative metabolic diseases of cows, adipose-derived stem cells (ADSCs) were eparated from perirenal adipose tissue of calves. ADSCs were collected by organization minced and 0.25% collagenase Ⅰ digestion by different times. The separated cells were proved by the following aspects, observed the morphology, passage 5 of ADSCs were used to detect the surface-specific proteins, passage 5 and 10 were used to detect the growth curve by CCK-8, passage 10 were used to detect the ability of adipogenic and osteogenic differentiation. The results showed that the ADSCs from calves were grown as fibroblast-like after 4 days of inoculation. Immunofluorescence staining showed that the isolated cells expressed Vimentin, CD34, CD44, and CD90, but did not express CD31 and CD45. The cells can be differentiated into adipocytes and osteoblasts under appropriate induction conditions. The separated cells were ADSCs with the ability of adipogenic and osteogenic differentiation.

Key words: calves, adipose stem cells, solation and cultivation, induced differentiation

CLC Number:

S828

WANG Shuaishuai, ZHANG Cai, MENG Sudan, HUANG Yongzhi, ZHU Xuemin, WANG Yuqin, YANG Zijun. Isolation, culture and identification of adipose-derived stem cells from calves[J]. , 2019, 31(2): 235-241.

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Isolation, culture and identification of adipose-derived stem cells from calves

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