Epidemiological investigation of Chinese sacbrood bee disease in honeybee (Apis cerana) in Chun'an County of Zhejiang Province, China

doi:10.3969/j.issn.1004-1524.2019.03.03

›› 2019, Vol. 31 ›› Issue (3): 365-371.DOI: 10.3969/j.issn.1004-1524.2019.03.03

• Animal Science • Previous Articles Next Articles

Epidemiological investigation of Chinese sacbrood bee disease in honeybee (Apis cerana) in Chun'an County of Zhejiang Province, China

DONG Jie¹, GUO Rui¹, GUO Haikun², LI Junzhu³, FANG Mengge⁴, WANG Deqian^1,*

1. Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;
2. Institute of Quality and Standard for Agro-Products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;
3. Hao Qingmi Beekeeping Professional Cooperative of Qiandao Lake, Zhejiang, Hangzhou 311701, China;
4. Fangxing Beekeeping Professional Cooperative of Qiandao Lake, Zhejiang, Hangzhou 311701, China

Received:2018-06-29 Online:2019-03-25 Published:2019-04-08

Abstract

Abstract: Chinese sacbrood bee disease is one of the serious diseases of Chinese honeybee, Apis cerana cerana. In this study, a RT-PCR method was developed to detect Chinese sacbrood bee virus (CSBV). Furthermore, an epidemiological investigation of CSBV in Chun'an County of Zhejiang Province was conducted based on the new method. The results showed that the developed RT-PCR method had a good repeatability, a sharp specificity and a high sensibility, whose minimum detecting concentration was 37 fg·mL^-1. The epidemiology of CSBV was investigated from 34 sample sites of 17 townships around Qiandao Lake area. The CSBV detection of 13 townships was positive, in which the infection rate was over 50 % at 6 towns. The high infection rate of CSBV may be related to the high density and mixture honeybee species keeping. In conclusion, the epidemic situation of Chinese sacbrood bee disease in Chun'an County was clarified and provided useful information for the disease control.

Key words: Chinese sacbrood bee disease, reverse transcription-polymerase chain reaction, epidemiology, Chun'an County;

CLC Number:

S895.1⁺33

DONG Jie, GUO Rui, GUO Haikun, LI Junzhu, FANG Mengge, WANG Deqian. Epidemiological investigation of Chinese sacbrood bee disease in honeybee (Apis cerana) in Chun'an County of Zhejiang Province, China[J]. , 2019, 31(3): 365-371.

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Epidemiological investigation of Chinese sacbrood bee disease in honeybee (Apis cerana) in Chun'an County of Zhejiang Province, China

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