Pathogen isolation and identification of blueberry stem canker and screening of disease resistant cultivars

doi:10.3969/j.issn.1004-1524.2019.03.13

›› 2019, Vol. 31 ›› Issue (3): 436-443.DOI: 10.3969/j.issn.1004-1524.2019.03.13

• Plant Protection • Previous Articles Next Articles

Pathogen isolation and identification of blueberry stem canker and screening of disease resistant cultivars

KANG Haiting¹, LING Danyan^1,2, WU Zhijuan¹, LU Mei¹, ZONG Yu¹, GUO Weidong^1,*

1. College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321004, China;
2. Jinhua Jinglong Biotechnology Company, Jinhua 321000, China

Received:2018-08-01 Online:2019-03-25 Published:2019-04-08

Abstract

Abstract: To verify the pathogens that lead to stem canker on blueberry, and provide a theoretical basis for field diagnosis and integrated control of the disease, the pathogens causing stem canker of blueberry were isolated and purified by tissue separation and single spore separation. Identification of pathogen were carried out using Koch's postulates, morphological characteristics and inter transcriptional spacer of ribosome DNA (rDNA-ITS). In vivo inoculation of pathogen and spot length measurement were performed to detect the resistance of five blueberry cultivars to stem canker. The test results showed that both strains JA4 and JB2 could cause stem canker on blueberries. ITS sequences analyses and colony identification revealed that strains JA4 and JB2 were Phomopsis sp. and Botryosphaeria dothidea, respectively. The inoculation results indicated that there was no significant difference in the resistance to stem canker among Brigitta, Bluecrop and Bluebelle. Both Phomopsis sp. and Botryosphaeria dothidea could cause stem canker on blueberry. Phomopsis sp. could be invaded by flower buds, and Botryosphaeria dothidea could be infected by the wound. The blueberry cultivars Brigitta, Bluecrop and Bluebelle showed higher resistance than Sharpblue, which was susceptible to infection by the pathogen and showed weaker resistance than other four cultivars.

Key words: blueberry, stem canker, Phomopsis, Botryosphaeria dothidea, resistance

CLC Number:

S436.63

KANG Haiting, LING Danyan, WU Zhijuan, LU Mei, ZONG Yu, GUO Weidong. Pathogen isolation and identification of blueberry stem canker and screening of disease resistant cultivars[J]. , 2019, 31(3): 436-443.

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Pathogen isolation and identification of blueberry stem canker and screening of disease resistant cultivars

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