Effects of retinol on in vitro biological characteristics of sertoli cells from prepubertal porcine testis

doi:10.3969/j.issn.1004-1524.2019.06.03

›› 2019, Vol. 31 ›› Issue (6): 878-885.DOI: 10.3969/j.issn.1004-1524.2019.06.03

• Animal Science • Previous Articles Next Articles

Effects of retinol on in vitro biological characteristics of sertoli cells from prepubertal porcine testis

DENG Jiaqiang, ZHONG Lijun, LIU Huanhuan, CAO Suizhong, SHEN Liuhong, YU Shumin^*

College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China

Received:2018-12-05 Online:2019-06-25 Published:2019-06-26

Abstract

Abstract: Sertoli cells are principal components of testicular seminiferous tubule epithelium. Retinol and its derivatives also play a critical role in the regulation of testicular development and spermatogenesis through sertoli cell mediation. To explore the effect of retinol on the in vitro biological characteristics of testicular sertoli cells, sertoli cells from 3 to 5 week-old porcine testis were selected as materials. Cellular viability, the expression of proliferation-related and apoptosis-related genes and secretion of cytokines were assessed. The results showed that compared with the control group, when the concentration of retinol was 5.000 and 0.125 μmol·L^-1, significant effects were seen on sertoli cell viability after 24 h and 72 h (P<0.05). Adding 1.250 μmol·L^-1 retinol group compared with the control group, the proliferation-related genes PCNA, BCL-2 and C-MYC were significantly down-regulated (P<0.05), apoptosis-related genes BAX was significantly up-regulated (P<0.05),and support intracellular GDNF, CSF-1 and EGF were significantly reduced in gene expression and protein levels (P<0.05). In summary, the addition of retinol can inhibit the activity of supporting cells and promote apoptosis in vitro culture conditions.

Key words: retinol, porcine, testis, sertoli cells

CLC Number:

S828

DENG Jiaqiang, ZHONG Lijun, LIU Huanhuan, CAO Suizhong, SHEN Liuhong, YU Shumin. Effects of retinol on in vitro biological characteristics of sertoli cells from prepubertal porcine testis[J]. , 2019, 31(6): 878-885.

References

[1] BENYA R V.The retinoids biology, chemistry, and medicine[J]. Journal of the American College of Nutrition, 1994, 13(6):675-676.
[2] CHIHARA M, OTSUKA S, ICHII O, et al.Vitamin A deprivation affects the progression of the spermatogenic wave and initial formation of the blood-testis barrier, resulting in irreversible testicular degeneration in mice[J]. Journal of Reproduction and Development, 2013, 59(6): 525-535.
[3] DUMONT L, OBLETTE A, RONDANINO C, et al.Vitamin A prevents round spermatid nuclear damage and promotes the production of motile sperm during in vitro maturation of vitrified pre-pubertal mouse testicular tissue[J]. Molecular Human Reproduction, 2016, 22(12):819-832.
[4] LIVERA G, ROUILLER-FABRE V, PAIRAULT C, et al.Regulation and perturbation of testicular functions by vitamin A[J]. Reproduction, 2002: 173-180.
[5] LI H, PALCZEWSKI K, BAEHR W, et al.Vitamin A deficiency results in meiotic failure and accumulation of undifferentiated spermatogonia in prepubertal mouse testis[J]. Biology of Reproduction, 2011, 84(2): 336-341.
[6] MRUK D D, CHENG C Y.Tight junctions in the testis: new perspectives[J]. Philosophical Transactions of the Royal Society B: Biological Sciences, 2010, 365(1546): 1621-1635.
[7] WONG C H, CHENG C Y.The blood-testis barrier: its biology, regulation, and physiological role in spermatogenesis[J]. Current Topics in Developmental Biology, 2005, 71: 263-296.
[8] ESCOTT G, DA ROSA L, LOSS E.Mechanisms of hormonal regulation of sertoli cell development and proliferation: a key process for spermatogenesis[J]. Current Molecular Pharmacology, 2015, 7(2): 96-108.
[9] REBOURCET D, WU J X, CRUICKSHANKS L, et al.Sertoli cells modulate testicular vascular network development, structure, and function to influence circulating testosterone concentrations in adult male mice[J]. Endocrinology, 2016, 157(6): 2479-2488.
[10] JOHNSON L, THOMPSON D L Jr, VARNER D D. Role of sertoli cell number and function on regulation of spermatogenesis[J]. Animal Reproduction Science, 2008, 105(1/2): 23-51.
[11] ALVES M G, RATO L, CARVALHO R A, et al.Hormonal control of sertoli cell metabolism regulates spermatogenesis[J]. Cellular and Molecular Life Sciences, 2013, 70(5): 777-793.
[12] HAI Y N, HOU J M, LIU Y, et al.The roles and regulation of sertoli cells in fate determinations of spermatogonial stem cells and spermatogenesis[J]. Seminars in Cell & Developmental Biology, 2014, 29: 66-75.
[13] KUBOTA H, AVARBOCK M R, BRINSTER R L.Growth factors essential for self-renewal and expansion of mouse spermatogonial stem cells[J]. Proceedings of the National Academy of Sciences, 2004, 101(47): 16489-16494.
[14] JOHNSTON D S, OLIVAS E, DICANDELORO P, et al.Stage-specific changes in GDNF expression by rat sertoli cells: a possible regulator of the replication and differentiation of stem spermatogonia1[J]. Biology of Reproduction, 2011, 85(4): 763-769.
[15] KADAM P H, KALA S, AGRAWAL H, et al.Effects of glial cell line-derived neurotrophic factor, fibroblast growth factor 2 and epidermal growth factor on proliferation and the expression of some genes in buffalo (Bubalus bubalis) spermatogonial cells[J]. Reproduction, Fertility and Development, 2013, 25(8): 1149-1157.
[16] WANG P, SUO L J, WANG Y F, et al.Effects of GDNF and LIF on mouse spermatogonial stem cells proliferation in vitro[J]. Cytotechnology, 2014, 66(2): 309-316.
[17] HAKOVIRTA H.Function of stem cell factor as a survival factor of spermatogonia and localization of messenger ribonucleic acid in the rat seminiferous epithelium[J]. Endocrinology, 1999, 140(3): 1492-1498.
[18] ZHANG Y, WANG S, WANG X X, et al.Endogenously produced FGF2 is essential for the survival and proliferation of cultured mouse spermatogonial stem cells[J]. Cell Research, 2012, 22(4): 773-776.
[19] 刘欢欢, 余树民, 夏娟, 等. 仔猪睾丸支持细胞的体外生物学特性研究[J]. 中国细胞生物学学报, 2015, 37(8): 1122-1128.
LIU H H, YU S M, XIA J, et al.Characterization of sertoli cells from prepubertal porcine testis in vitro[J]. Chinese Journal of Cell Biology, 2015, 37(8): 1122-1128.(in Chinese with English abstract)
[20] DAL-PIZZOL F, KLAMT F,FROTA M L C, et al.Retinol supplementation induces DNA damage and modulates iron turnover in rat Sertoli cells[J]. Free Radical Research, 2000, 33(5): 677-687.
[21] GELAIN D P,DE BITTENCOURT PASQUALI M A,ZANOTTO-FILHO A, et al.Retinol increases catalase activity and protein content by a reactive species-dependent mechanism in sertoli cells[J]. Chemico-Biological Interactions, 2008, 174(1): 38-43.
[22] OLIVEIRA R B, KLAMT F, CASTRO M A A, et al.Morphological and oxidative alterations on sertoli cells cytoskeleton due to retinol-induced reactive oxygen species[J]. Molecular and Cellular Biochemistry, 2005, 271(1/2): 189-196.
[23] MURATA M, KAWANISHI S.Oxidative DNA damage by vitamin A and its derivative via superoxide generation[J]. Journal of Biological Chemistry, 2000, 275(3): 2003-2008.
[24] BIDLACK W R.Retinoids: progress in research and clinical applications[J]. Journal of the American College of Nutrition, 1994, 13(6): 674-675.
[25] ROSS C.Cellular metabolism and activation of retinoids: roles of cellular retinoid-binding proteins[J]. The FASEB Journal, 1993, 7(2): 317-327.
[26] HALLIWELL B, GUTTERIDGE J M C. Free radicals in biology and medicine[J]. Journal of Free Radicals in Biology & Medicine, 1985, 1(4): 331-332.
[27] MARH J, TRES L L, YAMAZAKI Y, et al.Mouse round spermatids developed in vitro from preexisting spermatocytes can produce normal offspring by nuclear injection into in vivo-developed mature oocytes1[J]. Biology of Reproduction, 2003, 69(1): 169-176.
[28] JAN S Z, HAMER G, REPPING S, et al.Molecular control of rodent spermatogenesis[J]. Biochimica et Biophysica Acta, 2012, 1822(12): 1838-1850.

Effects of retinol on in vitro biological characteristics of sertoli cells from prepubertal porcine testis

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