Establishment of a multiplex PCR for porcine epidemic diarrhea virus and porcine circovirus type 2

doi:10.3969/j.issn.1004-1524.2017.07.05

›› 2017, Vol. 29 ›› Issue (7): 1086-1092.DOI: 10.3969/j.issn.1004-1524.2017.07.05

• Animal Science • Previous Articles Next Articles

Establishment of a multiplex PCR for porcine epidemic diarrhea virus and porcine circovirus type 2

GONG Shuangyan^a, LI Xiaojing^a, CHEN Yingqi^a, CAI Yao^a, LI Yumeng^a, XU Yifei^a, ZHU Ling^{a, b}, XU Zhiwen^{a, b, *}

a. College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China;
b. Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China

Received:2016-12-27 Online:2017-07-20 Published:2017-07-24

Abstract

Abstract: In order to study the infection status of porcine epidemic diarrhea virus (PEDV) and porcine circovirus type 2 (PCV2) in diarrhea piglets, a multiplex PCR method for simultaneous detection of PEDV and PCV2 mixed infection was established. According to the published sequence of PEDV and PCV2 in GenBank, 2 pairs of specific primers were designed and synthesized for the conserved region, and the amplified fragments were 530 and 278 bp, respectively. The multiplex PCR method for the specific detection of PEDV and PCV2 was established by optimizing the reaction conditions. A total of 67 diarrhea samples (mixed mesenteric lymph nodes, intestinal mucosa and their contents) were collected from Leshan, Yibin, Guangyuan and Suining of Sichuan Province from April to August, 2016, and were detected by multiplex PCR. The results showed that the single infection positive rates of PEDV and PCV2 was 34.33% and 73.13%, respectively. The combined infection rate of PEDV and PCV2 was 16.42%. The coincidence rates of the multiplex PCR method and the specific detection methods of PCV2 PCR and PEDV RT-PCR were all 100%.

Key words: porcine epidemic diarrhea virus, porcine circovirus type 2, multiplex PCR

CLC Number:

S855.3

GONG Shuangyan, LI Xiaojing, CHEN Yingqi, CAI Yao, LI Yumeng, XU Yifei, ZHU Ling, XU Zhiwen. Establishment of a multiplex PCR for porcine epidemic diarrhea virus and porcine circovirus type 2[J]. , 2017, 29(7): 1086-1092.

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Establishment of a multiplex PCR for porcine epidemic diarrhea virus and porcine circovirus type 2

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