Effects of single and combined exposure of deoxynivalenol and aflatoxin B1 on pathological changes, antioxidant capacity and tight junction protein expression of brain tissue in mice

doi:10.3969/j.issn.1004-1524.2019.06.04

›› 2019, Vol. 31 ›› Issue (6): 886-892.DOI: 10.3969/j.issn.1004-1524.2019.06.04

• Animal Science • Previous Articles Next Articles

Effects of single and combined exposure of deoxynivalenol and aflatoxin B₁ on pathological changes, antioxidant capacity and tight junction protein expression of brain tissue in mice

WANG Xichun¹, CAO Li¹, LIU Qin¹, DONG Yanli¹, WANG Zhongzheng^{1, 2}, LIU Jinfang¹, ZHU Lei¹, FENG Shibin¹, LI Yu¹, WU Jinjie¹

1. College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China;
2. Anhui Agriculture Committee of Tongling City, Tongling 244000, China

Received:2018-09-10 Online:2019-06-25 Published:2019-06-26

Abstract

Abstract: The present experiment was conducted to evaluate the effects of single and combined exposure of deoxynivalenol (DON) and aflatoxin B₁ (AFB₁) on pathological changes, antioxidant capacity and tight junction protein expression of brain tissues in mice. Ninety-six 18-day-old Kunming(male)mice were randomly divided into control group, DON group, AFB₁ group and AD group (DON+AFB₁ group). Each group was fed with physiological saline, 500 μg·kg^-1 DON, 200 μg·kg^-1 AFB₁ and 200 μg·kg^-1 AFB₁+500 μg·kg^-1 DON by gavage respectively. The trial was conducted for 45 d. On the days 0, 15, 30 and 45 of the experiment, six mice in each group were randomly selected and killed after anesthesia,the brain tissues were collected for observing pathological change and measuring the oxidative and antioxidant indexes and the mRNA expression of tight junction protein ZO-1 and Occludin of brain tissue in mice. The results showed that compared with the control group, the activities of superoxide dismutase (SOD) and catalase (CAT) in brain tissues of DON group were decreased significantly (P<0.05), while the content of NO was increased significantly (P<0.05). The activity of CAT in brain tissue of AFB₁ group decreased significantly (P<0.05). The activities of T-AOC, CAT and SOD of brain tissues in the AD group were decreased significantly (P<0.05). The contents of MDA and NO of brain tissues were increased significantly (P<0.05).The mRNA expression of ZO-1 and Occludin were decreased significantly in treatment group (P<0.05). In conclusion, single and combined exposure of DON and AFB₁ can lead to different degrees of pathological changes, as well as reduce antioxidant capacity in mouse brain tissue, produce oxidative damage, while decrease the mRNA expression of ZO-1 and Occludin. DON and AFB₁ exhibit a significant interaction with synergistic effects.

Key words: deoxynivalenol, aflatoxin B₁, combined toxicity, antioxidant capacity, tight junction protein

CLC Number:

S859

WANG Xichun, CAO Li, LIU Qin, DONG Yanli, WANG Zhongzheng, LIU Jinfang, ZHU Lei, FENG Shibin, LI Yu, WU Jinjie. Effects of single and combined exposure of deoxynivalenol and aflatoxin B₁ on pathological changes, antioxidant capacity and tight junction protein expression of brain tissue in mice[J]. , 2019, 31(6): 886-892.

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Effects of single and combined exposure of deoxynivalenol and aflatoxin B₁ on pathological changes, antioxidant capacity and tight junction protein expression of brain tissue in mice

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