Effects of high protein diets on serum uric acid levels, liver and kidney ultrastructure and expression of ABCG2 in goslings

doi:10.3969/j.issn.1004-1524.2021.05.04

Acta Agriculturae Zhejiangensis ›› 2021, Vol. 33 ›› Issue (5): 801-808.DOI: 10.3969/j.issn.1004-1524.2021.05.04

• Animal Science • Previous Articles Next Articles

Effects of high protein diets on serum uric acid levels, liver and kidney ultrastructure and expression of ABCG2 in goslings

WANG Zhi(), HU Zhonghao, GUI Xueer, FENG Shibin, LI Yu, WANG Xichun, LI Jinchun, WU Jinjie^*()

College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China

Received:2020-07-10 Online:2021-05-25 Published:2021-05-25
Contact: WU Jinjie

Abstract

Abstract:

In this study, the hyperuricemia model of gosling was established to study the effects of high protein diets on serum uric acid level, ultrastructure of liver and kidney and expression of ABCG2 in goslings.Transmission electron microscopy showed that the organelles structure of gosling liver and kidney in the control group (CP) was complete, whereas, some mitochondria of liver in high protein group 1 (HP1) and high protein group 2 (HP2) appeared to be swollen and showed vacuole degeneration, the endoplasmic reticulum was dilated, and glycogen levels were decreased. Some mitochondria of kidney cells appeared to be swollen, showed vacuole degeneration, and the nuclear membrane space increased. At 7 days of age, the serum uric acid levels in HP2 group were significantly higher than those of CP and HP1 groups (P<0.01). At 14 days of age, the serum uric acid level in HP2 group was significantly higher than those of CP and HP1 groups (P<0.01), and the serum uric acid level in HP1 group was significantly higher than those of CP group (P<0.01).The results of qPCR, Western-boltting and immunohistochemistry showed that, the expression of ABCG2 gene and protein in duodenum and jejunum in HP1 group was significantly higher than that in CP group and HP1 group (P<0.05 or P<0.01), and the expression in kidney was significantly lower than that in CP group and HP1 group (P<0.05 or P<0.01). The expression of ABCG2 gene and protein in HP1 group was significantly higher than that in CP group (P<0.05), and the expression in kidney was significantly lower than that in CP group (P<0.05). In conclusion, feeding high protein diet resulted in the damage of liver and kidney cells, the increase of uric acid level in vivo, the hyperuricemia of gosling, the increase of ABCG2 expression in jejunum and duodenum, and the decrease of ABCG2 expression in kidney.

Key words: high protein diet, hyperuricemia, ABCG2, gosling

CLC Number:

S835

WANG Zhi, HU Zhonghao, GUI Xueer, FENG Shibin, LI Yu, WANG Xichun, LI Jinchun, WU Jinjie. Effects of high protein diets on serum uric acid levels, liver and kidney ultrastructure and expression of ABCG2 in goslings[J]. Acta Agriculturae Zhejiangensis, 2021, 33(5): 801-808.

Figures/Tables 7

References 25

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项目 Items	含量 Content
项目 Items	对照组 CP		高蛋白1组 HP1		高蛋白2组 HP2
原料 Ingredients
玉米 Corn/%		32.52		30.33		19.52
豆粕 Soybean meal/%		—		21.39		13.70
玉米蛋白粉		6.06		9.40		13.30
Corn protein meal/%
花生粕 Peanut meal/%		8.64		—		14.60
青糠 Green bran/%		24.46		18.39		18.39
次粉 Wheat middling/%		15.23		9.67		9.67
酒糟蛋白饲料 DDGS/%		7.04		4.77		4.77
石粉 Limestone/%		2.10		2.10		2.10
磷酸氢钙 CaHPO₄/%		1.07		1.07		1.07
预混料 Premix/%		2.88		2.88		2.88
合计 Total/%		100		100		100
营养水平 Nutrient levels
粗蛋白 Crude protein/%		18		23		28
代谢能 ME/(MJ·kg^-1)		2.76		2.72		2.7
钙 Ca/%		1.05		1.05		1.05
总磷 TP/%		0.58		0.58		0.58
有效磷 AP/%		0.35		0.35		0.35
赖氨酸 Lys/%		0.81		1.26		1.26
蛋氨酸 Met/%		0.30		0.42		0.46
蛋氨酸+半胱氨酸		0.58		0.81		0.88
Met+Cys/%

项目 Items	含量 Content
项目 Items	对照组 CP		高蛋白1组 HP1		高蛋白2组 HP2
原料 Ingredients
玉米 Corn/%		32.52		30.33		19.52
豆粕 Soybean meal/%		—		21.39		13.70
玉米蛋白粉		6.06		9.40		13.30
Corn protein meal/%
花生粕 Peanut meal/%		8.64		—		14.60
青糠 Green bran/%		24.46		18.39		18.39
次粉 Wheat middling/%		15.23		9.67		9.67
酒糟蛋白饲料 DDGS/%		7.04		4.77		4.77
石粉 Limestone/%		2.10		2.10		2.10
磷酸氢钙 CaHPO₄/%		1.07		1.07		1.07
预混料 Premix/%		2.88		2.88		2.88
合计 Total/%		100		100		100
营养水平 Nutrient levels
粗蛋白 Crude protein/%		18		23		28
代谢能 ME/(MJ·kg^-1)		2.76		2.72		2.7
钙 Ca/%		1.05		1.05		1.05
总磷 TP/%		0.58		0.58		0.58
有效磷 AP/%		0.35		0.35		0.35
赖氨酸 Lys/%		0.81		1.26		1.26
蛋氨酸 Met/%		0.30		0.42		0.46
蛋氨酸+半胱氨酸		0.58		0.81		0.88
Met+Cys/%

处理 Treatment	血清尿酸值/(μmol·L^-1)
处理 Treatment	第7天 7th day	第14天 14th day
对照组CP	157.40±21.08 B	207.14±19.46 C
高蛋白1组HP1	201.72±27.66 B	452.58±60.26 B
高蛋白2组HP2	271.26±48.62 A	810.38±103.85 A

处理 Treatment	血清尿酸值/(μmol·L^-1)
处理 Treatment	第7天 7th day	第14天 14th day
对照组CP	157.40±21.08 B	207.14±19.46 C
高蛋白1组HP1	201.72±27.66 B	452.58±60.26 B
高蛋白2组HP2	271.26±48.62 A	810.38±103.85 A

Effects of high protein diets on serum uric acid levels, liver and kidney ultrastructure and expression of ABCG2 in goslings

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