Analysis of mRNA transport of BcSVP of Chinese flowering cabbage in heterograft

doi:10.3969/j.issn.1004-1524.2021.07.05

Acta Agriculturae Zhejiangensis ›› 2021, Vol. 33 ›› Issue (7): 1192-1198.DOI: 10.3969/j.issn.1004-1524.2021.07.05

• Horticultural Science • Previous Articles Next Articles

Analysis of mRNA transport of BcSVP of Chinese flowering cabbage in heterograft

TAO Peng(), ZHAO Yanting, YUE Zhichen, LEI Juanli, LI Biyuan^*()

Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2020-06-28 Online:2021-07-25 Published:2021-08-06
Contact: LI Biyuan

Abstract

Abstract:

For confirming mRNA long-transport of BcSVP gene, shoot apex of head cabbage was grafted on inflorescence stem of Chinese flowering cabbage to construct head cabbage-Chinese flowering cabbage heterograft. Based on transcriptome sequencing read library of Chinese flowering cabbage and head cabbage, coding sequences and 3’UTR sequences of BcSVP and BoSVP gene were obtained by read joining. According to interspecific differential sequences of BcSVP in 3’UTR (CF and CR), The two and one transported BcSVP reads of Chinese flowering cabbage were identified from transcriptome sequencing read libraries T01 and T02 of shoot apexes of head cabbage scions of head cabbage-Chinese flowering cabbage heterograft, respectively. The endogenous transcriptional expression of BoSVP was analyzed based on interspecific differential sequences of BoSVP(GF and GR), indicating the transcriptional expression of BoSVP did not show obvious difference between homograft and heterograft. The study indicated mRNA long-transport of BcSVP in heterograft. It lays the foundation for further study of molecule mechanism and biological function of BcSVP mRNA transport in head cabbage-Chinese flowering cabbage heterograft.

Key words: head cabbage, Chinese flowering cabbage, grafting, BcSVP, mRNA transport

CLC Number:

S635.1

TAO Peng, ZHAO Yanting, YUE Zhichen, LEI Juanli, LI Biyuan. Analysis of mRNA transport of BcSVP of Chinese flowering cabbage in heterograft[J]. Acta Agriculturae Zhejiangensis, 2021, 33(7): 1192-1198.

Figures/Tables 7

Fig.1 Sequence alignment of BcSVP and BoSVP from Chinese flowering cabbage and head cabbage Start codon and stop codon are marked by underline and box.

Table 1 Homologous gene and gene location of the SVP genes from Chinese flowering cabbage and head cabbage and isoelectric points and molecular weight of the corresponding coding proteins

基因名称 Gene name	物种 Species	同源基因定位 Homologous gene location	等电点 Isoelectric point	相对分子质量 Molecular weight/ku
BcSVP	菜心Chinese flowering cabbage	Bra030228(Chr04:10192172-10194736)	5.87	27.24
BoSVP	结球甘蓝head cabbage	Bo4g149800(Chr04:40723422-40726294)	5.87	27.29

Fig.2 Sequence alignment of 3’UTR of BcSVP and BoSVP from Chinese flowering cabbage and head cabbage Stop codon was marked by box. The retrieval sequence GF and CF were marked by black background and gray background respectively.

Fig.3 The three potential BcSVP reads of Chinese cabbage identified from T01 and T02 library of shoot apexes of head cabbage from heterograft by searching with CR sequence

Table 2 Related information of three potential BcSVP reads of Chinese cabbage identified from T01 and T02 library of shoot apexes of head cabbage from heterograft by searching with CR sequence

名称 Name	文库 Library	位置 Location	长度 Length/nt	3’UTR中的核苷酸/nt Nucleotides on 3'UTR/nt	与BcSVP差异核苷酸 Differential nucleotides with BcSVP	与BoSVP的差异核苷酸 Differential nucleotides with BoSVP
Read1	T01	34740498	150	149	0	27
Read2	T01	76178478	150	59	1	5
Read3	T02	59545522	150	71	3	7

Fig.4 Sequence alignment of long-transport reads, BoSVP and BcSVP from head cabbage and Chinese flowering cabbage The retrieval sequence CF and CR were marked by black background.

Table 3 The retrieval number in different libraries by searching with interspecific differential sequence

序列名称 Sequence name	序列 Sequence (5’-3’)	T01	T02	T03	T04	T05
CF	AGAAGTTCATGGAGTGAGGAGATGCTCTGTAGTAACAAGTG	0	0	0	0	44
CR	CACTTGTTACTACAGAGCATCTCCTCACTCCATGAACTTCT	2	1	0	0	88
GF	AGAAGTTGTGGAGTGAGGAGATACTCTGTGGTAACAAGTG	103	80	86	46	0
GR	CACTTGTTACCACAGAGTATCTCCTCACTCCACAACTTCT	200	185	103	113	0

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Analysis of mRNA transport of BcSVP of Chinese flowering cabbage in heterograft

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